Dissemination and Systemic Colonization of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> in a Murine Model of Bacteremia

ABSTRACT Infection with uropathogenic Escherichia coli (UPEC), the causative agent of most uncomplicated urinary tract infections, proceeds in an ascending manner and, if left untreated, may result in bacteremia and urosepsis. To examine the fate of UPEC after its entry into the bloodstream, we deve...

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Autores principales:	Sara N. Smith, Erin C. Hagan, M. Chelsea Lane, Harry L. T. Mobley
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2010
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a7a320376a0d48a4b04a71d830733b10
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spelling	oai:doaj.org-article:a7a320376a0d48a4b04a71d830733b102021-11-15T15:38:17ZDissemination and Systemic Colonization of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> in a Murine Model of Bacteremia10.1128/mBio.00262-102150-7511https://doaj.org/article/a7a320376a0d48a4b04a71d830733b102010-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00262-10https://doaj.org/toc/2150-7511ABSTRACT Infection with uropathogenic Escherichia coli (UPEC), the causative agent of most uncomplicated urinary tract infections, proceeds in an ascending manner and, if left untreated, may result in bacteremia and urosepsis. To examine the fate of UPEC after its entry into the bloodstream, we developed a murine model of sublethal bacteremia. CBA/J mice were inoculated intravenously with 1 × 106 CFU of pyelonephritis strain E. coli CFT073 carrying a bioluminescent reporter. Biophotonic imaging, used to monitor the infection over 48 h, demonstrated that the bacteria disseminated systemically and appeared to localize at discrete sites. UPEC was recovered from the spleen, liver, kidneys, lungs, heart, brain, and intestines as early as 20 min postinoculation, peaking at 24 h postinoculation. A nonpathogenic E. coli K-12 strain, however, disseminated at significantly lower levels (P < 0.01) and was cleared from the liver and cecum by 24 h postinoculation. Isogenic mutants lacking type 1 fimbriae, P fimbriae, capsule, TonB, the heme receptors Hma and ChuA, or particularly the sialic acid catabolism enzyme NanA were significantly outcompeted by wild-type CFT073 during bacteremia (P < 0.05), while flagellin and hemolysin mutants were not. IMPORTANCE E. coli is the primary cause of urinary tract infections. In severe cases of kidney infection, bacteria can enter the bloodstream and cause systemic disease. While the ability of E. coli to cause urinary tract infection has been extensively studied, the fate of these bacteria once they enter the bloodstream is largely unknown. Here we used an imaging technique to develop a mouse model of E. coli bloodstream infection and identify bacterial genes that are important for the bacteria to spread to and infect various organs. Understanding how urinary tract pathogens like E. coli cause disease after they enter the bloodstream may aid in the development of protective and therapeutic treatments.Sara N. SmithErin C. HaganM. Chelsea LaneHarry L. T. MobleyAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 1, Iss 5 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Sara N. Smith Erin C. Hagan M. Chelsea Lane Harry L. T. Mobley Dissemination and Systemic Colonization of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> in a Murine Model of Bacteremia
description	ABSTRACT Infection with uropathogenic Escherichia coli (UPEC), the causative agent of most uncomplicated urinary tract infections, proceeds in an ascending manner and, if left untreated, may result in bacteremia and urosepsis. To examine the fate of UPEC after its entry into the bloodstream, we developed a murine model of sublethal bacteremia. CBA/J mice were inoculated intravenously with 1 × 106 CFU of pyelonephritis strain E. coli CFT073 carrying a bioluminescent reporter. Biophotonic imaging, used to monitor the infection over 48 h, demonstrated that the bacteria disseminated systemically and appeared to localize at discrete sites. UPEC was recovered from the spleen, liver, kidneys, lungs, heart, brain, and intestines as early as 20 min postinoculation, peaking at 24 h postinoculation. A nonpathogenic E. coli K-12 strain, however, disseminated at significantly lower levels (P < 0.01) and was cleared from the liver and cecum by 24 h postinoculation. Isogenic mutants lacking type 1 fimbriae, P fimbriae, capsule, TonB, the heme receptors Hma and ChuA, or particularly the sialic acid catabolism enzyme NanA were significantly outcompeted by wild-type CFT073 during bacteremia (P < 0.05), while flagellin and hemolysin mutants were not. IMPORTANCE E. coli is the primary cause of urinary tract infections. In severe cases of kidney infection, bacteria can enter the bloodstream and cause systemic disease. While the ability of E. coli to cause urinary tract infection has been extensively studied, the fate of these bacteria once they enter the bloodstream is largely unknown. Here we used an imaging technique to develop a mouse model of E. coli bloodstream infection and identify bacterial genes that are important for the bacteria to spread to and infect various organs. Understanding how urinary tract pathogens like E. coli cause disease after they enter the bloodstream may aid in the development of protective and therapeutic treatments.
format	article
author	Sara N. Smith Erin C. Hagan M. Chelsea Lane Harry L. T. Mobley
author_facet	Sara N. Smith Erin C. Hagan M. Chelsea Lane Harry L. T. Mobley
author_sort	Sara N. Smith
title	Dissemination and Systemic Colonization of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> in a Murine Model of Bacteremia
title_short	Dissemination and Systemic Colonization of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> in a Murine Model of Bacteremia
title_full	Dissemination and Systemic Colonization of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> in a Murine Model of Bacteremia
title_fullStr	Dissemination and Systemic Colonization of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> in a Murine Model of Bacteremia
title_full_unstemmed	Dissemination and Systemic Colonization of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> in a Murine Model of Bacteremia
title_sort	dissemination and systemic colonization of uropathogenic <named-content content-type="genus-species">escherichia coli</named-content> in a murine model of bacteremia
publisher	American Society for Microbiology
publishDate	2010
url	https://doaj.org/article/a7a320376a0d48a4b04a71d830733b10
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Dissemination and Systemic Colonization of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> in a Murine Model of Bacteremia

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