Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions

The cellular cytoskeleton provides the cell with a mechanical rigidity that allows mechanical interaction between cells and the extracellular environment. The actin structure plays a key role in mechanical events such as motility or the establishment of cell polarity. From the earliest stages of dev...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Younes F. Barooji, Kasper G. Hvid, Irene Istúriz Petitjean, Joshua M. Brickman, Lene B. Oddershede, Poul M. Bendix
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	actin cytoskeleton super-resolution microscopy (STORM) embryonic stem cells primed embryonic stem cells micro-rheology cell culturing Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a7a3bdb97c8d4c66a9b705b854a9fb01
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a7a3bdb97c8d4c66a9b705b854a9fb01
record_format	dspace
spelling	oai:doaj.org-article:a7a3bdb97c8d4c66a9b705b854a9fb012021-11-25T17:08:10ZChanges in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions10.3390/cells101128592073-4409https://doaj.org/article/a7a3bdb97c8d4c66a9b705b854a9fb012021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2859https://doaj.org/toc/2073-4409The cellular cytoskeleton provides the cell with a mechanical rigidity that allows mechanical interaction between cells and the extracellular environment. The actin structure plays a key role in mechanical events such as motility or the establishment of cell polarity. From the earliest stages of development, as represented by the ex vivo expansion of naïve embryonic stem cells (ESCs), the critical mechanical role of the actin structure is becoming recognized as a vital cue for correct segregation and lineage control of cells and as a regulatory structure that controls several transcription factors. Naïve ESCs have a characteristic morphology, and the ultrastructure that underlies this condition remains to be further investigated. Here, we investigate the 3D actin cytoskeleton of naïve mouse ESCs using super-resolution optical reconstruction microscopy (STORM). We investigate the morphological, cytoskeletal, and mechanical changes in cells cultured in 2i or Serum/LIF media reflecting, respectively, a homogeneous preimplantation cell state and a state that is closer to embarking on differentiation. STORM imaging showed that the peripheral actin structure undergoes a dramatic change between the two culturing conditions. We also detected micro-rheological differences in the cell periphery between the cells cultured in these two media correlating well with the observed nano-architecture of the ESCs in the two different culture conditions. These results pave the way for linking physical properties and cytoskeletal architecture to cell morphology during early development.Younes F. BaroojiKasper G. HvidIrene Istúriz PetitjeanJoshua M. BrickmanLene B. OddershedePoul M. BendixMDPI AGarticleactin cytoskeletonsuper-resolution microscopy (STORM)embryonic stem cellsprimed embryonic stem cellsmicro-rheologycell culturingBiology (General)QH301-705.5ENCells, Vol 10, Iss 2859, p 2859 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	actin cytoskeleton super-resolution microscopy (STORM) embryonic stem cells primed embryonic stem cells micro-rheology cell culturing Biology (General) QH301-705.5
spellingShingle	actin cytoskeleton super-resolution microscopy (STORM) embryonic stem cells primed embryonic stem cells micro-rheology cell culturing Biology (General) QH301-705.5 Younes F. Barooji Kasper G. Hvid Irene Istúriz Petitjean Joshua M. Brickman Lene B. Oddershede Poul M. Bendix Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions
description	The cellular cytoskeleton provides the cell with a mechanical rigidity that allows mechanical interaction between cells and the extracellular environment. The actin structure plays a key role in mechanical events such as motility or the establishment of cell polarity. From the earliest stages of development, as represented by the ex vivo expansion of naïve embryonic stem cells (ESCs), the critical mechanical role of the actin structure is becoming recognized as a vital cue for correct segregation and lineage control of cells and as a regulatory structure that controls several transcription factors. Naïve ESCs have a characteristic morphology, and the ultrastructure that underlies this condition remains to be further investigated. Here, we investigate the 3D actin cytoskeleton of naïve mouse ESCs using super-resolution optical reconstruction microscopy (STORM). We investigate the morphological, cytoskeletal, and mechanical changes in cells cultured in 2i or Serum/LIF media reflecting, respectively, a homogeneous preimplantation cell state and a state that is closer to embarking on differentiation. STORM imaging showed that the peripheral actin structure undergoes a dramatic change between the two culturing conditions. We also detected micro-rheological differences in the cell periphery between the cells cultured in these two media correlating well with the observed nano-architecture of the ESCs in the two different culture conditions. These results pave the way for linking physical properties and cytoskeletal architecture to cell morphology during early development.
format	article
author	Younes F. Barooji Kasper G. Hvid Irene Istúriz Petitjean Joshua M. Brickman Lene B. Oddershede Poul M. Bendix
author_facet	Younes F. Barooji Kasper G. Hvid Irene Istúriz Petitjean Joshua M. Brickman Lene B. Oddershede Poul M. Bendix
author_sort	Younes F. Barooji
title	Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions
title_short	Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions
title_full	Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions
title_fullStr	Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions
title_full_unstemmed	Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions
title_sort	changes in cell morphology and actin organization in embryonic stem cells cultured under different conditions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a7a3bdb97c8d4c66a9b705b854a9fb01
work_keys_str_mv	AT younesfbarooji changesincellmorphologyandactinorganizationinembryonicstemcellsculturedunderdifferentconditions AT kasperghvid changesincellmorphologyandactinorganizationinembryonicstemcellsculturedunderdifferentconditions AT ireneisturizpetitjean changesincellmorphologyandactinorganizationinembryonicstemcellsculturedunderdifferentconditions AT joshuambrickman changesincellmorphologyandactinorganizationinembryonicstemcellsculturedunderdifferentconditions AT leneboddershede changesincellmorphologyandactinorganizationinembryonicstemcellsculturedunderdifferentconditions AT poulmbendix changesincellmorphologyandactinorganizationinembryonicstemcellsculturedunderdifferentconditions
_version_	1718412732059877376

Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured under Different Conditions

Ejemplares similares