Alterations in gut microbiota linked to provenance, sex, and chronic wasting disease in white-tailed deer (Odocoileus virginianus)

Abstract Chronic wasting disease (CWD) is a fatal, contagious, neurodegenerative prion disease affecting both free-ranging and captive cervid species. CWD is spread via direct or indirect contact or oral ingestion of prions. In the gastrointestinal tract, prions enter the body through microfold cell...

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Autores principales:	David Minich, Christopher Madden, Morgan V. Evans, Gregory A. Ballash, Daniel J. Barr, Keith P. Poulsen, Patricia M. Dennis, Vanessa L. Hale
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7a831b9f9e24437b22fe7d44921d420
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spelling	oai:doaj.org-article:a7a831b9f9e24437b22fe7d44921d4202021-12-02T17:12:21ZAlterations in gut microbiota linked to provenance, sex, and chronic wasting disease in white-tailed deer (Odocoileus virginianus)10.1038/s41598-021-89896-92045-2322https://doaj.org/article/a7a831b9f9e24437b22fe7d44921d4202021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89896-9https://doaj.org/toc/2045-2322Abstract Chronic wasting disease (CWD) is a fatal, contagious, neurodegenerative prion disease affecting both free-ranging and captive cervid species. CWD is spread via direct or indirect contact or oral ingestion of prions. In the gastrointestinal tract, prions enter the body through microfold cells (M-cells), and the abundance of these cells can be influenced by the gut microbiota. To explore potential links between the gut microbiota and CWD, we collected fecal samples from farmed and free-ranging white-tailed deer (Odocoileus virginianus) around the Midwest, USA. Farmed deer originated from farms that were depopulated due to CWD. Free-ranging deer were sampled during annual deer harvests. All farmed deer were tested for CWD via ELISA and IHC, and we used 16S rRNA gene sequencing to characterize the gut microbiota. We report significant differences in gut microbiota by provenance (Farm 1, Farm 2, Free-ranging), sex, and CWD status. CWD-positive deer from Farm 1 and 2 had increased abundances of Akkermansia, Lachnospireacea UCG-010, and RF39 taxa. Overall, differences by provenance and sex appear to be driven by diet, while differences by CWD status may be linked to CWD pathogenesis.David MinichChristopher MaddenMorgan V. EvansGregory A. BallashDaniel J. BarrKeith P. PoulsenPatricia M. DennisVanessa L. HaleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David Minich Christopher Madden Morgan V. Evans Gregory A. Ballash Daniel J. Barr Keith P. Poulsen Patricia M. Dennis Vanessa L. Hale Alterations in gut microbiota linked to provenance, sex, and chronic wasting disease in white-tailed deer (Odocoileus virginianus)
description	Abstract Chronic wasting disease (CWD) is a fatal, contagious, neurodegenerative prion disease affecting both free-ranging and captive cervid species. CWD is spread via direct or indirect contact or oral ingestion of prions. In the gastrointestinal tract, prions enter the body through microfold cells (M-cells), and the abundance of these cells can be influenced by the gut microbiota. To explore potential links between the gut microbiota and CWD, we collected fecal samples from farmed and free-ranging white-tailed deer (Odocoileus virginianus) around the Midwest, USA. Farmed deer originated from farms that were depopulated due to CWD. Free-ranging deer were sampled during annual deer harvests. All farmed deer were tested for CWD via ELISA and IHC, and we used 16S rRNA gene sequencing to characterize the gut microbiota. We report significant differences in gut microbiota by provenance (Farm 1, Farm 2, Free-ranging), sex, and CWD status. CWD-positive deer from Farm 1 and 2 had increased abundances of Akkermansia, Lachnospireacea UCG-010, and RF39 taxa. Overall, differences by provenance and sex appear to be driven by diet, while differences by CWD status may be linked to CWD pathogenesis.
format	article
author	David Minich Christopher Madden Morgan V. Evans Gregory A. Ballash Daniel J. Barr Keith P. Poulsen Patricia M. Dennis Vanessa L. Hale
author_facet	David Minich Christopher Madden Morgan V. Evans Gregory A. Ballash Daniel J. Barr Keith P. Poulsen Patricia M. Dennis Vanessa L. Hale
author_sort	David Minich
title	Alterations in gut microbiota linked to provenance, sex, and chronic wasting disease in white-tailed deer (Odocoileus virginianus)
title_short	Alterations in gut microbiota linked to provenance, sex, and chronic wasting disease in white-tailed deer (Odocoileus virginianus)
title_full	Alterations in gut microbiota linked to provenance, sex, and chronic wasting disease in white-tailed deer (Odocoileus virginianus)
title_fullStr	Alterations in gut microbiota linked to provenance, sex, and chronic wasting disease in white-tailed deer (Odocoileus virginianus)
title_full_unstemmed	Alterations in gut microbiota linked to provenance, sex, and chronic wasting disease in white-tailed deer (Odocoileus virginianus)
title_sort	alterations in gut microbiota linked to provenance, sex, and chronic wasting disease in white-tailed deer (odocoileus virginianus)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a7a831b9f9e24437b22fe7d44921d420
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Alterations in gut microbiota linked to provenance, sex, and chronic wasting disease in white-tailed deer (Odocoileus virginianus)

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