Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus.

Brain aquaporin 1 (AQP1) and AQP4 are involved in cerebrospinal fluid (CSF) homeostasis and might participate in the origin of hydrocephalus. Studies have shown alterations of perivascular AQP4 expression in idiopathic normal pressure hydrocephalus (iNPH) and Alzheimer's disease (AD). Due to th...

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Autores principales:	Laura Hiraldo-González, José Luis Trillo-Contreras, Pablo García-Miranda, Rocío Pineda-Sánchez, Reposo Ramírez-Lorca, Silvia Rodrigo-Herrero, Magdalena Olivares Blanco, María Oliver, Maria Bernal, Emilio Franco-Macías, Javier Villadiego, Miriam Echevarría
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7aa9949cf3f4f38945eee48a909b1ca
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spelling	oai:doaj.org-article:a7aa9949cf3f4f38945eee48a909b1ca2021-12-02T20:17:23ZEvaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus.1932-620310.1371/journal.pone.0258165https://doaj.org/article/a7aa9949cf3f4f38945eee48a909b1ca2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258165https://doaj.org/toc/1932-6203Brain aquaporin 1 (AQP1) and AQP4 are involved in cerebrospinal fluid (CSF) homeostasis and might participate in the origin of hydrocephalus. Studies have shown alterations of perivascular AQP4 expression in idiopathic normal pressure hydrocephalus (iNPH) and Alzheimer's disease (AD). Due to the overlapping of clinical signs between iNPH and certain neurological conditions, mainly AD, specific biomarkers might improve the diagnostic accuracy for iNPH. The goal of the present study was to analyze and quantify the presence of AQP1 and AQP4 in the CSF of patients with iNPH and AD to determine whether these proteins can be used as biomarkers of iNPH. We examined AQP1 and AQP4 protein levels in the CSF of 179 participants (88 women) classified into 5 groups: possible iNPH (81 participants), hydrocephalus associated with other neurological disorders (13 participants), AD (41 participants), non-AD dementia (32 participants) and healthy controls (12 participants). We recorded each participant's demographic and clinical variables and indicated, when available in the clinical history, the record of cardiovascular and respiratory complications. An ELISA showed virtually no AQP content in the CSF. Information on the vascular risk factors (available for 61 patients) confirmed some type of vascular risk factor in 86% of the patients with possible iNPH and 58% of the patients with AD. In conclusion, the ELISA analysis showed insufficient sensitivity to detect the presence of AQP1 and AQP4 in CSF, ruling out the possible use of these proteins as biomarkers for diagnosing iNPH.Laura Hiraldo-GonzálezJosé Luis Trillo-ContrerasPablo García-MirandaRocío Pineda-SánchezReposo Ramírez-LorcaSilvia Rodrigo-HerreroMagdalena Olivares BlancoMaría OliverMaria BernalEmilio Franco-MacíasJavier VilladiegoMiriam EchevarríaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258165 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Hiraldo-González José Luis Trillo-Contreras Pablo García-Miranda Rocío Pineda-Sánchez Reposo Ramírez-Lorca Silvia Rodrigo-Herrero Magdalena Olivares Blanco María Oliver Maria Bernal Emilio Franco-Macías Javier Villadiego Miriam Echevarría Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus.
description	Brain aquaporin 1 (AQP1) and AQP4 are involved in cerebrospinal fluid (CSF) homeostasis and might participate in the origin of hydrocephalus. Studies have shown alterations of perivascular AQP4 expression in idiopathic normal pressure hydrocephalus (iNPH) and Alzheimer's disease (AD). Due to the overlapping of clinical signs between iNPH and certain neurological conditions, mainly AD, specific biomarkers might improve the diagnostic accuracy for iNPH. The goal of the present study was to analyze and quantify the presence of AQP1 and AQP4 in the CSF of patients with iNPH and AD to determine whether these proteins can be used as biomarkers of iNPH. We examined AQP1 and AQP4 protein levels in the CSF of 179 participants (88 women) classified into 5 groups: possible iNPH (81 participants), hydrocephalus associated with other neurological disorders (13 participants), AD (41 participants), non-AD dementia (32 participants) and healthy controls (12 participants). We recorded each participant's demographic and clinical variables and indicated, when available in the clinical history, the record of cardiovascular and respiratory complications. An ELISA showed virtually no AQP content in the CSF. Information on the vascular risk factors (available for 61 patients) confirmed some type of vascular risk factor in 86% of the patients with possible iNPH and 58% of the patients with AD. In conclusion, the ELISA analysis showed insufficient sensitivity to detect the presence of AQP1 and AQP4 in CSF, ruling out the possible use of these proteins as biomarkers for diagnosing iNPH.
format	article
author	Laura Hiraldo-González José Luis Trillo-Contreras Pablo García-Miranda Rocío Pineda-Sánchez Reposo Ramírez-Lorca Silvia Rodrigo-Herrero Magdalena Olivares Blanco María Oliver Maria Bernal Emilio Franco-Macías Javier Villadiego Miriam Echevarría
author_facet	Laura Hiraldo-González José Luis Trillo-Contreras Pablo García-Miranda Rocío Pineda-Sánchez Reposo Ramírez-Lorca Silvia Rodrigo-Herrero Magdalena Olivares Blanco María Oliver Maria Bernal Emilio Franco-Macías Javier Villadiego Miriam Echevarría
author_sort	Laura Hiraldo-González
title	Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus.
title_short	Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus.
title_full	Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus.
title_fullStr	Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus.
title_full_unstemmed	Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus.
title_sort	evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a7aa9949cf3f4f38945eee48a909b1ca
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Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus.

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