In Vitro Adventitious Regeneration of Artemisia annua L. Influencing Artemisinin Metabolism

Artemisia annua L. is a herbaceous plant belonging to the Asteraceae family, known for producing, although at low levels, the sesquiterpene lactone artemisinin (AN), which is highly effective against malaria. In this study, an in vitro regeneration process of &l...

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Autores principales:	Federica Blando, Francesca Rizzello, Miriana Durante, Angelo De Paolis, Sofia Caretto, Giovanni Mita
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Artemisia annua</i> organogenesis in vitro regeneration artemisinin metabolism micropropagation Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/a7b22a5b1ec34870afa2856dffa6e665
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spelling	oai:doaj.org-article:a7b22a5b1ec34870afa2856dffa6e6652021-11-25T17:47:08ZIn Vitro Adventitious Regeneration of <i>Artemisia annua</i> L. Influencing Artemisinin Metabolism10.3390/horticulturae71104382311-7524https://doaj.org/article/a7b22a5b1ec34870afa2856dffa6e6652021-10-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/438https://doaj.org/toc/2311-7524<i>Artemisia annua</i> L. is a herbaceous plant belonging to the <i>Asteraceae</i> family, known for producing, although at low levels, the sesquiterpene lactone artemisinin (AN), which is highly effective against malaria. In this study, an in vitro regeneration process of <i>A. annua</i> L. using ‘Artemis’ progeny was established and the potential of tissue culture for inducing new variability in terms of AN metabolism of in vitro regenerated plants was investigated. Among the plant growth regulators tested, the cytokinin 6-benzyladenine (BA) at 4.4 μM in combination with the auxin indole-butyric acid (IBA) at 0.35 μM yielded the greatest frequency of shoot induction. The optimal multiplication medium contained BA at 0.9 μM and naphthaleneacetic acid (NAA) at 0.05 μM. Regenerated plants (RPs), after transferring to the greenhouse and subsequently to the field, were analyzed during the growth cycle at different sampling times, showing a peak of AN content 20 days before blossom. Variability among different RPs and sampling times, in terms of AN and its precursors dihydroartemisinic acid (DHAA) and artemisinic acid (AA) was observed. This suggests that adventitious shoot induction could provide a useful strategy to induce variability influencing artemisinin metabolism as a consequence of in vitro manipulation.Federica BlandoFrancesca RizzelloMiriana DuranteAngelo De PaolisSofia CarettoGiovanni MitaMDPI AGarticle<i>Artemisia annua</i>organogenesisin vitro regenerationartemisinin metabolismmicropropagationPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 438, p 438 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Artemisia annua</i> organogenesis in vitro regeneration artemisinin metabolism micropropagation Plant culture SB1-1110
spellingShingle	<i>Artemisia annua</i> organogenesis in vitro regeneration artemisinin metabolism micropropagation Plant culture SB1-1110 Federica Blando Francesca Rizzello Miriana Durante Angelo De Paolis Sofia Caretto Giovanni Mita In Vitro Adventitious Regeneration of <i>Artemisia annua</i> L. Influencing Artemisinin Metabolism
description	<i>Artemisia annua</i> L. is a herbaceous plant belonging to the <i>Asteraceae</i> family, known for producing, although at low levels, the sesquiterpene lactone artemisinin (AN), which is highly effective against malaria. In this study, an in vitro regeneration process of <i>A. annua</i> L. using ‘Artemis’ progeny was established and the potential of tissue culture for inducing new variability in terms of AN metabolism of in vitro regenerated plants was investigated. Among the plant growth regulators tested, the cytokinin 6-benzyladenine (BA) at 4.4 μM in combination with the auxin indole-butyric acid (IBA) at 0.35 μM yielded the greatest frequency of shoot induction. The optimal multiplication medium contained BA at 0.9 μM and naphthaleneacetic acid (NAA) at 0.05 μM. Regenerated plants (RPs), after transferring to the greenhouse and subsequently to the field, were analyzed during the growth cycle at different sampling times, showing a peak of AN content 20 days before blossom. Variability among different RPs and sampling times, in terms of AN and its precursors dihydroartemisinic acid (DHAA) and artemisinic acid (AA) was observed. This suggests that adventitious shoot induction could provide a useful strategy to induce variability influencing artemisinin metabolism as a consequence of in vitro manipulation.
format	article
author	Federica Blando Francesca Rizzello Miriana Durante Angelo De Paolis Sofia Caretto Giovanni Mita
author_facet	Federica Blando Francesca Rizzello Miriana Durante Angelo De Paolis Sofia Caretto Giovanni Mita
author_sort	Federica Blando
title	In Vitro Adventitious Regeneration of <i>Artemisia annua</i> L. Influencing Artemisinin Metabolism
title_short	In Vitro Adventitious Regeneration of <i>Artemisia annua</i> L. Influencing Artemisinin Metabolism
title_full	In Vitro Adventitious Regeneration of <i>Artemisia annua</i> L. Influencing Artemisinin Metabolism
title_fullStr	In Vitro Adventitious Regeneration of <i>Artemisia annua</i> L. Influencing Artemisinin Metabolism
title_full_unstemmed	In Vitro Adventitious Regeneration of <i>Artemisia annua</i> L. Influencing Artemisinin Metabolism
title_sort	in vitro adventitious regeneration of <i>artemisia annua</i> l. influencing artemisinin metabolism
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a7b22a5b1ec34870afa2856dffa6e665
work_keys_str_mv	AT federicablando invitroadventitiousregenerationofiartemisiaannuailinfluencingartemisininmetabolism AT francescarizzello invitroadventitiousregenerationofiartemisiaannuailinfluencingartemisininmetabolism AT mirianadurante invitroadventitiousregenerationofiartemisiaannuailinfluencingartemisininmetabolism AT angelodepaolis invitroadventitiousregenerationofiartemisiaannuailinfluencingartemisininmetabolism AT sofiacaretto invitroadventitiousregenerationofiartemisiaannuailinfluencingartemisininmetabolism AT giovannimita invitroadventitiousregenerationofiartemisiaannuailinfluencingartemisininmetabolism
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In Vitro Adventitious Regeneration of <i>Artemisia annua</i> L. Influencing Artemisinin Metabolism

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