Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride

In this study, we have developed two facile spectroscopic methods for quantifying oxybutynin (OBT) hydrochloride in its pure form and tablets using design of experiments (DOEs). The spectroscopic methods depended on the ion-pair complex formation between the tertiary amino group in the drug and eosi...

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Autores principales:	Eman Yosrey, Heba Elmansi, Zeinab A. Sheribah, Mohamed El-Sayed Metwally
Formato:	article
Lenguaje:	EN
Publicado:	The Royal Society 2021
Materias:	oxybutynin hydrochloride eosin Y DOE green Science Q
Acceso en línea:	https://doaj.org/article/a7b4e2ca40da48e6be01d1e925866463
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spelling	oai:doaj.org-article:a7b4e2ca40da48e6be01d1e9258664632021-11-24T08:05:47ZFactorial design-assisted spectroscopic determination of oxybutynin hydrochloride10.1098/rsos.2110272054-5703https://doaj.org/article/a7b4e2ca40da48e6be01d1e9258664632021-11-01T00:00:00Zhttps://royalsocietypublishing.org/doi/10.1098/rsos.211027https://doaj.org/toc/2054-5703In this study, we have developed two facile spectroscopic methods for quantifying oxybutynin (OBT) hydrochloride in its pure form and tablets using design of experiments (DOEs). The spectroscopic methods depended on the ion-pair complex formation between the tertiary amino group in the drug and eosin in 0.2 M acetate buffer of pH 4. Method I involves spectrophotometric measurement of the absorbance of the developed complex at 550 nm and showed linearity through 1.0–10.0 µg ml−1. Method II involves spectrofluorometric measurement of the quenching influence of OBT on the native fluorescence of eosin (λ excitation/λ emission of 304/548 nm) and showed linearity through 1.0–6.0 µg ml−1. Critical parameters were identified through preliminary trials and optimized using the DOE. Additionally, the quenching mechanism was investigated and the pathway of the reaction was postulated. The fluorescence quenching constant and thermodynamic parameters were explored using the Stern–Volmer plot and Van't Hoff graph, respectively. Assessments conducted via analytical ecoscale revealed the ‘excellent-greenness’ of the methodology. The two methods have the potentials of being green and fast compared with other reported methods.Eman YosreyHeba ElmansiZeinab A. SheribahMohamed El-Sayed MetwallyThe Royal Societyarticleoxybutynin hydrochlorideeosin YDOEgreenScienceQENRoyal Society Open Science, Vol 8, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oxybutynin hydrochloride eosin Y DOE green Science Q
spellingShingle	oxybutynin hydrochloride eosin Y DOE green Science Q Eman Yosrey Heba Elmansi Zeinab A. Sheribah Mohamed El-Sayed Metwally Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride
description	In this study, we have developed two facile spectroscopic methods for quantifying oxybutynin (OBT) hydrochloride in its pure form and tablets using design of experiments (DOEs). The spectroscopic methods depended on the ion-pair complex formation between the tertiary amino group in the drug and eosin in 0.2 M acetate buffer of pH 4. Method I involves spectrophotometric measurement of the absorbance of the developed complex at 550 nm and showed linearity through 1.0–10.0 µg ml−1. Method II involves spectrofluorometric measurement of the quenching influence of OBT on the native fluorescence of eosin (λ excitation/λ emission of 304/548 nm) and showed linearity through 1.0–6.0 µg ml−1. Critical parameters were identified through preliminary trials and optimized using the DOE. Additionally, the quenching mechanism was investigated and the pathway of the reaction was postulated. The fluorescence quenching constant and thermodynamic parameters were explored using the Stern–Volmer plot and Van't Hoff graph, respectively. Assessments conducted via analytical ecoscale revealed the ‘excellent-greenness’ of the methodology. The two methods have the potentials of being green and fast compared with other reported methods.
format	article
author	Eman Yosrey Heba Elmansi Zeinab A. Sheribah Mohamed El-Sayed Metwally
author_facet	Eman Yosrey Heba Elmansi Zeinab A. Sheribah Mohamed El-Sayed Metwally
author_sort	Eman Yosrey
title	Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride
title_short	Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride
title_full	Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride
title_fullStr	Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride
title_full_unstemmed	Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride
title_sort	factorial design-assisted spectroscopic determination of oxybutynin hydrochloride
publisher	The Royal Society
publishDate	2021
url	https://doaj.org/article/a7b4e2ca40da48e6be01d1e925866463
work_keys_str_mv	AT emanyosrey factorialdesignassistedspectroscopicdeterminationofoxybutyninhydrochloride AT hebaelmansi factorialdesignassistedspectroscopicdeterminationofoxybutyninhydrochloride AT zeinabasheribah factorialdesignassistedspectroscopicdeterminationofoxybutyninhydrochloride AT mohamedelsayedmetwally factorialdesignassistedspectroscopicdeterminationofoxybutyninhydrochloride
_version_	1718415816337129472

Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride

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