Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice

Abstract The cholinergic efferent network from the medial septal nucleus to the hippocampus plays an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to encode novel information. Hippocampal cholinergic neurostimulating p...

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Autores principales:	Yuko Kondo-Takuma, Masayuki Mizuno, Yo Tsuda, Yuta Madokoro, Kengo Suzuki, Toyohiro Sato, Hiroshi Takase, Yuto Uchida, Ken-ichi Adachi, Hideki Hida, Cesario V. Borlongan, Noriyuki Matsukawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7b5a2b3cdd8482c82247c81b0a68170
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spelling	oai:doaj.org-article:a7b5a2b3cdd8482c82247c81b0a681702021-11-14T12:22:17ZReduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice10.1038/s41598-021-01667-82045-2322https://doaj.org/article/a7b5a2b3cdd8482c82247c81b0a681702021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01667-8https://doaj.org/toc/2045-2322Abstract The cholinergic efferent network from the medial septal nucleus to the hippocampus plays an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP), which induces acetylcholine (Ach) synthesis in the medial septal nuclei of an explant culture system, was purified from the soluble fraction of postnatal rat hippocampus. HCNP is processed from the N-terminal region of a 186-amino acid, 21-kDa HCNP precursor protein, also known as Raf kinase inhibitory protein and phosphatidylethanolamine-binding protein 1. Here, we confirmed direct reduction of Ach release in the hippocampus of freely moving HCNP-pp knockout mice under an arousal state by the microdialysis method. The levels of vesicular acetylcholine transporter were also decreased in the hippocampus of these mice in comparison with those in control mice, suggesting there was decreased incorporation of Ach into the synaptic vesicle. These results potently indicate that HCNP may be a cholinergic regulator in the septo-hippocampal network.Yuko Kondo-TakumaMasayuki MizunoYo TsudaYuta MadokoroKengo SuzukiToyohiro SatoHiroshi TakaseYuto UchidaKen-ichi AdachiHideki HidaCesario V. BorlonganNoriyuki MatsukawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuko Kondo-Takuma Masayuki Mizuno Yo Tsuda Yuta Madokoro Kengo Suzuki Toyohiro Sato Hiroshi Takase Yuto Uchida Ken-ichi Adachi Hideki Hida Cesario V. Borlongan Noriyuki Matsukawa Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
description	Abstract The cholinergic efferent network from the medial septal nucleus to the hippocampus plays an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP), which induces acetylcholine (Ach) synthesis in the medial septal nuclei of an explant culture system, was purified from the soluble fraction of postnatal rat hippocampus. HCNP is processed from the N-terminal region of a 186-amino acid, 21-kDa HCNP precursor protein, also known as Raf kinase inhibitory protein and phosphatidylethanolamine-binding protein 1. Here, we confirmed direct reduction of Ach release in the hippocampus of freely moving HCNP-pp knockout mice under an arousal state by the microdialysis method. The levels of vesicular acetylcholine transporter were also decreased in the hippocampus of these mice in comparison with those in control mice, suggesting there was decreased incorporation of Ach into the synaptic vesicle. These results potently indicate that HCNP may be a cholinergic regulator in the septo-hippocampal network.
format	article
author	Yuko Kondo-Takuma Masayuki Mizuno Yo Tsuda Yuta Madokoro Kengo Suzuki Toyohiro Sato Hiroshi Takase Yuto Uchida Ken-ichi Adachi Hideki Hida Cesario V. Borlongan Noriyuki Matsukawa
author_facet	Yuko Kondo-Takuma Masayuki Mizuno Yo Tsuda Yuta Madokoro Kengo Suzuki Toyohiro Sato Hiroshi Takase Yuto Uchida Ken-ichi Adachi Hideki Hida Cesario V. Borlongan Noriyuki Matsukawa
author_sort	Yuko Kondo-Takuma
title	Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
title_short	Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
title_full	Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
title_fullStr	Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
title_full_unstemmed	Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
title_sort	reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a7b5a2b3cdd8482c82247c81b0a68170
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Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice

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