Hsa_circ_0001666 suppresses the progression of colorectal cancer through the miR‐576‐5p/PCDH10 axis
Abstract Background Though circular RNAs, new non‐coding RNA classes have demonstrated that they have an essential role in the initiation as well as development of CRC (colorectal cancer), whereas in CRC the function and mechanism of hsa_circ_0001666 are less known. Methods Hsa_circ_0001666 was iden...
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Wiley
2021
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oai:doaj.org-article:a7b6e0e3a7944614bd79062320e3ff1e2021-11-30T07:25:37ZHsa_circ_0001666 suppresses the progression of colorectal cancer through the miR‐576‐5p/PCDH10 axis2001-132610.1002/ctm2.565https://doaj.org/article/a7b6e0e3a7944614bd79062320e3ff1e2021-11-01T00:00:00Zhttps://doi.org/10.1002/ctm2.565https://doaj.org/toc/2001-1326Abstract Background Though circular RNAs, new non‐coding RNA classes have demonstrated that they have an essential role in the initiation as well as development of CRC (colorectal cancer), whereas in CRC the function and mechanism of hsa_circ_0001666 are less known. Methods Hsa_circ_0001666 was identified by bioinformatics analysis of a circRNA microarray from the GEO database, and its expression in both CRC cell lines and tissues was analysed. A series of in vitro along with in vivo experiments were carried out for exploring the hsa_circ_0001666 functions, including transwell, wound healing, flow cytometry, colony formation, Edu, CCK‐8, soft agar colony formation, tumor xenografts and lung/liver metastasis in mice. RNA pull‐down, RIP (RNA immunoprecipitation), luciferase reporter assay, FISH (fluorescence in situ hybridization) and rescue experiments were used for determining the correlation among hsa_circ_0001666, miR‐576‐5p and PCDH10. Results Hsa_circ_0001666 was downregulated in both CRC cell lines along with tumour tissues. A higher expression level of hsa_circ_0001666 indicated a better clinical prognosis in patients with CRC. Hsa_circ_0001666 knockdown significantly supported CRC cell proliferation along with invasion and inhibited cell apoptosis in vitro. Hsa_circ_0001666 knockdown accelerated the CRC growth and metastasis in vivo. Moreover, the mechanistic study showed that hsa_circ_0001666, acting as ‘ceRNA’ of miR‐576‐5p, prevented PCDH10 downregulation, as well as suppressed EMT and stemness of CRC cells, and the Wnt/β‐catenin signalling pathway. Inhibiting miR‐576‐5p or overexpressing PCDH10 could reverse phenotypic changes caused by knocking down of hsa_circ_0001666. Conclusions Hsa_circ_0001666 suppresses CRC progression through the miR‐576‐5p/PCDH10 axis and may provide a new insight for the diagnosis and treatment of CRC.Jiahui ZhouLu WangQingyang SunRanran ChenChuan ZhangPeng YangYuqian TanChaofan PengTuo WangChi JinJiangzhou JiKangpeng JinYueming SunWileyarticleceRNAcolorectal cancerhsa_circ_0001666miR‐576‐5pPCDH10Medicine (General)R5-920ENClinical and Translational Medicine, Vol 11, Iss 11, Pp n/a-n/a (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
ceRNA colorectal cancer hsa_circ_0001666 miR‐576‐5p PCDH10 Medicine (General) R5-920 |
| spellingShingle |
ceRNA colorectal cancer hsa_circ_0001666 miR‐576‐5p PCDH10 Medicine (General) R5-920 Jiahui Zhou Lu Wang Qingyang Sun Ranran Chen Chuan Zhang Peng Yang Yuqian Tan Chaofan Peng Tuo Wang Chi Jin Jiangzhou Ji Kangpeng Jin Yueming Sun Hsa_circ_0001666 suppresses the progression of colorectal cancer through the miR‐576‐5p/PCDH10 axis |
| description |
Abstract Background Though circular RNAs, new non‐coding RNA classes have demonstrated that they have an essential role in the initiation as well as development of CRC (colorectal cancer), whereas in CRC the function and mechanism of hsa_circ_0001666 are less known. Methods Hsa_circ_0001666 was identified by bioinformatics analysis of a circRNA microarray from the GEO database, and its expression in both CRC cell lines and tissues was analysed. A series of in vitro along with in vivo experiments were carried out for exploring the hsa_circ_0001666 functions, including transwell, wound healing, flow cytometry, colony formation, Edu, CCK‐8, soft agar colony formation, tumor xenografts and lung/liver metastasis in mice. RNA pull‐down, RIP (RNA immunoprecipitation), luciferase reporter assay, FISH (fluorescence in situ hybridization) and rescue experiments were used for determining the correlation among hsa_circ_0001666, miR‐576‐5p and PCDH10. Results Hsa_circ_0001666 was downregulated in both CRC cell lines along with tumour tissues. A higher expression level of hsa_circ_0001666 indicated a better clinical prognosis in patients with CRC. Hsa_circ_0001666 knockdown significantly supported CRC cell proliferation along with invasion and inhibited cell apoptosis in vitro. Hsa_circ_0001666 knockdown accelerated the CRC growth and metastasis in vivo. Moreover, the mechanistic study showed that hsa_circ_0001666, acting as ‘ceRNA’ of miR‐576‐5p, prevented PCDH10 downregulation, as well as suppressed EMT and stemness of CRC cells, and the Wnt/β‐catenin signalling pathway. Inhibiting miR‐576‐5p or overexpressing PCDH10 could reverse phenotypic changes caused by knocking down of hsa_circ_0001666. Conclusions Hsa_circ_0001666 suppresses CRC progression through the miR‐576‐5p/PCDH10 axis and may provide a new insight for the diagnosis and treatment of CRC. |
| format |
article |
| author |
Jiahui Zhou Lu Wang Qingyang Sun Ranran Chen Chuan Zhang Peng Yang Yuqian Tan Chaofan Peng Tuo Wang Chi Jin Jiangzhou Ji Kangpeng Jin Yueming Sun |
| author_facet |
Jiahui Zhou Lu Wang Qingyang Sun Ranran Chen Chuan Zhang Peng Yang Yuqian Tan Chaofan Peng Tuo Wang Chi Jin Jiangzhou Ji Kangpeng Jin Yueming Sun |
| author_sort |
Jiahui Zhou |
| title |
Hsa_circ_0001666 suppresses the progression of colorectal cancer through the miR‐576‐5p/PCDH10 axis |
| title_short |
Hsa_circ_0001666 suppresses the progression of colorectal cancer through the miR‐576‐5p/PCDH10 axis |
| title_full |
Hsa_circ_0001666 suppresses the progression of colorectal cancer through the miR‐576‐5p/PCDH10 axis |
| title_fullStr |
Hsa_circ_0001666 suppresses the progression of colorectal cancer through the miR‐576‐5p/PCDH10 axis |
| title_full_unstemmed |
Hsa_circ_0001666 suppresses the progression of colorectal cancer through the miR‐576‐5p/PCDH10 axis |
| title_sort |
hsa_circ_0001666 suppresses the progression of colorectal cancer through the mir‐576‐5p/pcdh10 axis |
| publisher |
Wiley |
| publishDate |
2021 |
| url |
https://doaj.org/article/a7b6e0e3a7944614bd79062320e3ff1e |
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