Diversity of vaginal microbiome and metabolome during genital infections

Abstract We characterized the vaginal ecosystem during common infections of the female genital tract, as vulvovaginal candidiasis (VVC, n = 18) and Chlamydia trachomatis infection (CT, n = 20), recruiting healthy (HC, n = 21) and bacterial vaginosis-affected (BV, n = 20) women as references of eubio...

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Autores principales:	Camilla Ceccarani, Claudio Foschi, Carola Parolin, Antonietta D’Antuono, Valeria Gaspari, Clarissa Consolandi, Luca Laghi, Tania Camboni, Beatrice Vitali, Marco Severgnini, Antonella Marangoni
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7c01a84c5cb46f1908ddc935a2ce9be
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spelling	oai:doaj.org-article:a7c01a84c5cb46f1908ddc935a2ce9be2021-12-02T15:10:04ZDiversity of vaginal microbiome and metabolome during genital infections10.1038/s41598-019-50410-x2045-2322https://doaj.org/article/a7c01a84c5cb46f1908ddc935a2ce9be2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-50410-xhttps://doaj.org/toc/2045-2322Abstract We characterized the vaginal ecosystem during common infections of the female genital tract, as vulvovaginal candidiasis (VVC, n = 18) and Chlamydia trachomatis infection (CT, n = 20), recruiting healthy (HC, n = 21) and bacterial vaginosis-affected (BV, n = 20) women as references of eubiosis and dysbiosis. The profiles of the vaginal microbiome and metabolome were studied in 79 reproductive-aged women, by means of next generation sequencing and proton based-nuclear magnetic resonance spectroscopy. Lactobacillus genus was profoundly depleted in all the genital infections herein considered, and species-level analysis revealed that healthy vaginal microbiome was dominated by L. crispatus. In the shift from HC to CT, VVC, and BV, L. crispatus was progressively replaced by L. iners. CT infection and VVC, as well as BV condition, were mainly characterised by anaerobe genera, e.g. Gardnerella, Prevotella, Megasphaera, Roseburia and Atopobium. The changes in the bacterial communities occurring during the genital infections resulted in significant alterations in the vaginal metabolites composition, being the decrease of lactate a common marker of all the pathological conditions. In conclusion, according to the taxonomic and metabolomics analysis, we found that each of the four conditions is characterized by a peculiar vaginal microbiome/metabolome fingerprint.Camilla CeccaraniClaudio FoschiCarola ParolinAntonietta D’AntuonoValeria GaspariClarissa ConsolandiLuca LaghiTania CamboniBeatrice VitaliMarco SevergniniAntonella MarangoniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Camilla Ceccarani Claudio Foschi Carola Parolin Antonietta D’Antuono Valeria Gaspari Clarissa Consolandi Luca Laghi Tania Camboni Beatrice Vitali Marco Severgnini Antonella Marangoni Diversity of vaginal microbiome and metabolome during genital infections
description	Abstract We characterized the vaginal ecosystem during common infections of the female genital tract, as vulvovaginal candidiasis (VVC, n = 18) and Chlamydia trachomatis infection (CT, n = 20), recruiting healthy (HC, n = 21) and bacterial vaginosis-affected (BV, n = 20) women as references of eubiosis and dysbiosis. The profiles of the vaginal microbiome and metabolome were studied in 79 reproductive-aged women, by means of next generation sequencing and proton based-nuclear magnetic resonance spectroscopy. Lactobacillus genus was profoundly depleted in all the genital infections herein considered, and species-level analysis revealed that healthy vaginal microbiome was dominated by L. crispatus. In the shift from HC to CT, VVC, and BV, L. crispatus was progressively replaced by L. iners. CT infection and VVC, as well as BV condition, were mainly characterised by anaerobe genera, e.g. Gardnerella, Prevotella, Megasphaera, Roseburia and Atopobium. The changes in the bacterial communities occurring during the genital infections resulted in significant alterations in the vaginal metabolites composition, being the decrease of lactate a common marker of all the pathological conditions. In conclusion, according to the taxonomic and metabolomics analysis, we found that each of the four conditions is characterized by a peculiar vaginal microbiome/metabolome fingerprint.
format	article
author	Camilla Ceccarani Claudio Foschi Carola Parolin Antonietta D’Antuono Valeria Gaspari Clarissa Consolandi Luca Laghi Tania Camboni Beatrice Vitali Marco Severgnini Antonella Marangoni
author_facet	Camilla Ceccarani Claudio Foschi Carola Parolin Antonietta D’Antuono Valeria Gaspari Clarissa Consolandi Luca Laghi Tania Camboni Beatrice Vitali Marco Severgnini Antonella Marangoni
author_sort	Camilla Ceccarani
title	Diversity of vaginal microbiome and metabolome during genital infections
title_short	Diversity of vaginal microbiome and metabolome during genital infections
title_full	Diversity of vaginal microbiome and metabolome during genital infections
title_fullStr	Diversity of vaginal microbiome and metabolome during genital infections
title_full_unstemmed	Diversity of vaginal microbiome and metabolome during genital infections
title_sort	diversity of vaginal microbiome and metabolome during genital infections
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/a7c01a84c5cb46f1908ddc935a2ce9be
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Diversity of vaginal microbiome and metabolome during genital infections

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