Conditional control of fluorescent protein degradation by an auxin-dependent nanobody

Current approaches to conditionally deplete target proteins require site-specific genetic engineering or have poor temporal control. Here the authors overcome these limitations by combining the AID system with nanobodies to reversibly degrade GFP-tagged proteins in living cells and zebrafish.

Guardado en:

Detalles Bibliográficos
Autores principales:	Katrin Daniel, Jaroslav Icha, Cindy Horenburg, Doris Müller, Caren Norden, Jörg Mansfeld
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a7c05273d2474b3cb400cd105f73eb4a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a7c05273d2474b3cb400cd105f73eb4a
record_format	dspace
spelling	oai:doaj.org-article:a7c05273d2474b3cb400cd105f73eb4a2021-12-02T17:32:02ZConditional control of fluorescent protein degradation by an auxin-dependent nanobody10.1038/s41467-018-05855-52041-1723https://doaj.org/article/a7c05273d2474b3cb400cd105f73eb4a2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05855-5https://doaj.org/toc/2041-1723Current approaches to conditionally deplete target proteins require site-specific genetic engineering or have poor temporal control. Here the authors overcome these limitations by combining the AID system with nanobodies to reversibly degrade GFP-tagged proteins in living cells and zebrafish.Katrin DanielJaroslav IchaCindy HorenburgDoris MüllerCaren NordenJörg MansfeldNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Katrin Daniel Jaroslav Icha Cindy Horenburg Doris Müller Caren Norden Jörg Mansfeld Conditional control of fluorescent protein degradation by an auxin-dependent nanobody
description	Current approaches to conditionally deplete target proteins require site-specific genetic engineering or have poor temporal control. Here the authors overcome these limitations by combining the AID system with nanobodies to reversibly degrade GFP-tagged proteins in living cells and zebrafish.
format	article
author	Katrin Daniel Jaroslav Icha Cindy Horenburg Doris Müller Caren Norden Jörg Mansfeld
author_facet	Katrin Daniel Jaroslav Icha Cindy Horenburg Doris Müller Caren Norden Jörg Mansfeld
author_sort	Katrin Daniel
title	Conditional control of fluorescent protein degradation by an auxin-dependent nanobody
title_short	Conditional control of fluorescent protein degradation by an auxin-dependent nanobody
title_full	Conditional control of fluorescent protein degradation by an auxin-dependent nanobody
title_fullStr	Conditional control of fluorescent protein degradation by an auxin-dependent nanobody
title_full_unstemmed	Conditional control of fluorescent protein degradation by an auxin-dependent nanobody
title_sort	conditional control of fluorescent protein degradation by an auxin-dependent nanobody
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a7c05273d2474b3cb400cd105f73eb4a
work_keys_str_mv	AT katrindaniel conditionalcontroloffluorescentproteindegradationbyanauxindependentnanobody AT jaroslavicha conditionalcontroloffluorescentproteindegradationbyanauxindependentnanobody AT cindyhorenburg conditionalcontroloffluorescentproteindegradationbyanauxindependentnanobody AT dorismuller conditionalcontroloffluorescentproteindegradationbyanauxindependentnanobody AT carennorden conditionalcontroloffluorescentproteindegradationbyanauxindependentnanobody AT jorgmansfeld conditionalcontroloffluorescentproteindegradationbyanauxindependentnanobody
_version_	1718380447145132032

Conditional control of fluorescent protein degradation by an auxin-dependent nanobody

Ejemplares similares