Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework

Abstract A sensitive, selective and recyclable electrochemical sensor is designed for ATP detection based on amino-functionalized metal-organic framework. The functional MOF as the sensor is constructed by one-step synthesis Ce-MOF and sequentially modified on the Au electrode and conjugated with th...

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Autores principales:	Pengfei Shi, Yuanchao Zhang, Zhaopeng Yu, Shusheng Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7c264c44fff42cd8a0fa47109044d33
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spelling	oai:doaj.org-article:a7c264c44fff42cd8a0fa47109044d332021-12-02T12:31:53ZLabel-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework10.1038/s41598-017-06858-w2045-2322https://doaj.org/article/a7c264c44fff42cd8a0fa47109044d332017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06858-whttps://doaj.org/toc/2045-2322Abstract A sensitive, selective and recyclable electrochemical sensor is designed for ATP detection based on amino-functionalized metal-organic framework. The functional MOF as the sensor is constructed by one-step synthesis Ce-MOF and sequentially modified on the Au electrode and conjugated with the aptamer of ATP. The presence of target ATP leads to the conformational change of aptamer strands and strong electrochemical impedance. The electrochemical sensor can detect ATP down to 5.6 nM with the linear range of 10 nm to 1000 μM. The present study is the first report on the use of MOF as an electrochemical sensor for ATP at nM level. This strategy has been successfully applied in detection of ATP in serum of cancer patients, which reveals its potential application in clinical diagnosis.Pengfei ShiYuanchao ZhangZhaopeng YuShusheng ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pengfei Shi Yuanchao Zhang Zhaopeng Yu Shusheng Zhang Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
description	Abstract A sensitive, selective and recyclable electrochemical sensor is designed for ATP detection based on amino-functionalized metal-organic framework. The functional MOF as the sensor is constructed by one-step synthesis Ce-MOF and sequentially modified on the Au electrode and conjugated with the aptamer of ATP. The presence of target ATP leads to the conformational change of aptamer strands and strong electrochemical impedance. The electrochemical sensor can detect ATP down to 5.6 nM with the linear range of 10 nm to 1000 μM. The present study is the first report on the use of MOF as an electrochemical sensor for ATP at nM level. This strategy has been successfully applied in detection of ATP in serum of cancer patients, which reveals its potential application in clinical diagnosis.
format	article
author	Pengfei Shi Yuanchao Zhang Zhaopeng Yu Shusheng Zhang
author_facet	Pengfei Shi Yuanchao Zhang Zhaopeng Yu Shusheng Zhang
author_sort	Pengfei Shi
title	Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
title_short	Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
title_full	Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
title_fullStr	Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
title_full_unstemmed	Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework
title_sort	label-free electrochemical detection of atp based on amino-functionalized metal-organic framework
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a7c264c44fff42cd8a0fa47109044d33
work_keys_str_mv	AT pengfeishi labelfreeelectrochemicaldetectionofatpbasedonaminofunctionalizedmetalorganicframework AT yuanchaozhang labelfreeelectrochemicaldetectionofatpbasedonaminofunctionalizedmetalorganicframework AT zhaopengyu labelfreeelectrochemicaldetectionofatpbasedonaminofunctionalizedmetalorganicframework AT shushengzhang labelfreeelectrochemicaldetectionofatpbasedonaminofunctionalizedmetalorganicframework
_version_	1718394233273974784

Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework

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