SARS-CoV-2 Infection in One Cat and Three Dogs Living in COVID-19-Positive Households in Madrid, Spain

In this study, we describe SARS-CoV-2 infection dynamics in one cat and three dogs from households with confirmed human cases of COVID-19 living in the Madrid Community (Spain) at the time of expansion (December 2020 through June 2021) of the alpha variant (lineage B.1.1.7). A thorough physical exam...

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Autores principales:	Guadalupe Miró, Javier Regidor-Cerrillo, Rocio Checa, Carlos Diezma-Díaz, Ana Montoya, Jesús García-Cantalejo, Pedro Botías, Javier Arroyo, Luis-Miguel Ortega-Mora
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cat dog SARS-CoV-2 infection COVID-19-positive households Spain alpha variant Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/a7c2cca854e2478699e5ca966a64e9af
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spelling	oai:doaj.org-article:a7c2cca854e2478699e5ca966a64e9af2021-11-10T06:08:21ZSARS-CoV-2 Infection in One Cat and Three Dogs Living in COVID-19-Positive Households in Madrid, Spain2297-176910.3389/fvets.2021.779341https://doaj.org/article/a7c2cca854e2478699e5ca966a64e9af2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.779341/fullhttps://doaj.org/toc/2297-1769In this study, we describe SARS-CoV-2 infection dynamics in one cat and three dogs from households with confirmed human cases of COVID-19 living in the Madrid Community (Spain) at the time of expansion (December 2020 through June 2021) of the alpha variant (lineage B.1.1.7). A thorough physical exam and nasopharyngeal, oropharyngeal, and rectal swabs were collected for real-time reverse-transcription PCR (RT-qPCR) SARS-CoV-2 testing on day 0 and in successive samplings on days 7, 14, 21, and 47 during monitoring. Blood was also drawn to determine complete blood counts, biochemical profiles, and serology of the IgG response against SARS-CoV-2. On day 0, the cat case 1 presented with dyspnea and fever associated with a mild bronchoalveolar pattern. The dog cases 2, 3, and 4 were healthy, but case 2 presented with coughing, dyspnea, and weakness, and case 4 exhibited coughing and bilateral nasal discharge 3 and 6 days before the clinical exam. Case 3 (from the same household as case 2) remained asymptomatic. SARS-CoV-2 detection by RT-qPCR showed that the cat case 1 and the dog case 2 exhibited the lowest cycle threshold (Ct) (Ct < 30) when they presented clinical signs. Viral detection failed in successive samplings. Serological analyses revealed a positive IgG response in cat case 1 and dog cases 3 and 4 shortly after or simultaneously to virus shedding. Dog case 2 was seronegative, but seroconverted 21 days after SARS-CoV-2 detection. SARS-CoV-2 genome sequencing was attempted, and genomes were classified as belonging to the B.1.1.7 lineage.Guadalupe MiróJavier Regidor-CerrilloRocio ChecaCarlos Diezma-DíazAna MontoyaJesús García-CantalejoPedro BotíasJavier ArroyoLuis-Miguel Ortega-MoraFrontiers Media S.A.articlecatdogSARS-CoV-2 infectionCOVID-19-positive householdsSpainalpha variantVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cat dog SARS-CoV-2 infection COVID-19-positive households Spain alpha variant Veterinary medicine SF600-1100
spellingShingle	cat dog SARS-CoV-2 infection COVID-19-positive households Spain alpha variant Veterinary medicine SF600-1100 Guadalupe Miró Javier Regidor-Cerrillo Rocio Checa Carlos Diezma-Díaz Ana Montoya Jesús García-Cantalejo Pedro Botías Javier Arroyo Luis-Miguel Ortega-Mora SARS-CoV-2 Infection in One Cat and Three Dogs Living in COVID-19-Positive Households in Madrid, Spain
description	In this study, we describe SARS-CoV-2 infection dynamics in one cat and three dogs from households with confirmed human cases of COVID-19 living in the Madrid Community (Spain) at the time of expansion (December 2020 through June 2021) of the alpha variant (lineage B.1.1.7). A thorough physical exam and nasopharyngeal, oropharyngeal, and rectal swabs were collected for real-time reverse-transcription PCR (RT-qPCR) SARS-CoV-2 testing on day 0 and in successive samplings on days 7, 14, 21, and 47 during monitoring. Blood was also drawn to determine complete blood counts, biochemical profiles, and serology of the IgG response against SARS-CoV-2. On day 0, the cat case 1 presented with dyspnea and fever associated with a mild bronchoalveolar pattern. The dog cases 2, 3, and 4 were healthy, but case 2 presented with coughing, dyspnea, and weakness, and case 4 exhibited coughing and bilateral nasal discharge 3 and 6 days before the clinical exam. Case 3 (from the same household as case 2) remained asymptomatic. SARS-CoV-2 detection by RT-qPCR showed that the cat case 1 and the dog case 2 exhibited the lowest cycle threshold (Ct) (Ct < 30) when they presented clinical signs. Viral detection failed in successive samplings. Serological analyses revealed a positive IgG response in cat case 1 and dog cases 3 and 4 shortly after or simultaneously to virus shedding. Dog case 2 was seronegative, but seroconverted 21 days after SARS-CoV-2 detection. SARS-CoV-2 genome sequencing was attempted, and genomes were classified as belonging to the B.1.1.7 lineage.
format	article
author	Guadalupe Miró Javier Regidor-Cerrillo Rocio Checa Carlos Diezma-Díaz Ana Montoya Jesús García-Cantalejo Pedro Botías Javier Arroyo Luis-Miguel Ortega-Mora
author_facet	Guadalupe Miró Javier Regidor-Cerrillo Rocio Checa Carlos Diezma-Díaz Ana Montoya Jesús García-Cantalejo Pedro Botías Javier Arroyo Luis-Miguel Ortega-Mora
author_sort	Guadalupe Miró
title	SARS-CoV-2 Infection in One Cat and Three Dogs Living in COVID-19-Positive Households in Madrid, Spain
title_short	SARS-CoV-2 Infection in One Cat and Three Dogs Living in COVID-19-Positive Households in Madrid, Spain
title_full	SARS-CoV-2 Infection in One Cat and Three Dogs Living in COVID-19-Positive Households in Madrid, Spain
title_fullStr	SARS-CoV-2 Infection in One Cat and Three Dogs Living in COVID-19-Positive Households in Madrid, Spain
title_full_unstemmed	SARS-CoV-2 Infection in One Cat and Three Dogs Living in COVID-19-Positive Households in Madrid, Spain
title_sort	sars-cov-2 infection in one cat and three dogs living in covid-19-positive households in madrid, spain
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a7c2cca854e2478699e5ca966a64e9af
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SARS-CoV-2 Infection in One Cat and Three Dogs Living in COVID-19-Positive Households in Madrid, Spain

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