SuhB Is a Regulator of Multiple Virulence Genes and Essential for Pathogenesis of <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

ABSTRACT During initial colonization and chronic infection, pathogenic bacteria encounter distinct host environments. Adjusting gene expression accordingly is essential for the pathogenesis. Pseudomonas aeruginosa has evolved complicated regulatory networks to regulate different sets of virulence fa...

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Autores principales:	Kewei Li, Chang Xu, Yongxin Jin, Ziyu Sun, Chang Liu, Jing Shi, Gukui Chen, Ronghao Chen, Shouguang Jin, Weihui Wu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a7c2d93515bb4bc0a0af15f10e66689a
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spelling	oai:doaj.org-article:a7c2d93515bb4bc0a0af15f10e66689a2021-11-15T15:42:32ZSuhB Is a Regulator of Multiple Virulence Genes and Essential for Pathogenesis of <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>10.1128/mBio.00419-132150-7511https://doaj.org/article/a7c2d93515bb4bc0a0af15f10e66689a2013-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00419-13https://doaj.org/toc/2150-7511ABSTRACT During initial colonization and chronic infection, pathogenic bacteria encounter distinct host environments. Adjusting gene expression accordingly is essential for the pathogenesis. Pseudomonas aeruginosa has evolved complicated regulatory networks to regulate different sets of virulence factors to facilitate colonization and persistence. The type III secretion system (T3SS) and motility are associated with acute infections, while biofilm formation and the type VI secretion system (T6SS) are associated with chronic persistence. To identify novel regulatory genes required for pathogenesis, we screened a P. aeruginosa transposon (Tn) insertion library and found suhB to be an essential gene for the T3SS gene expression. The expression of suhB was upregulated in a mouse acute lung infection model, and loss of suhB resulted in avirulence. Suppression of T3SS gene expression in the suhB mutant is linked to a defective translation of the T3SS master regulator, ExsA. Further studies demonstrated that suhB mutation led to the upregulation of GacA and its downstream small RNAs, RsmY and RsmZ, triggering T6SS expression and biofilm formation while inhibiting the T3SS. Our results demonstrate that an in vivo-inducible gene, suhB, reciprocally regulates genes associated with acute and chronic infections and plays an essential role in the pathogenesis of P. aeruginosa. IMPORTANCE A variety of bacterial pathogens, such as Pseudomonas aeruginosa, cause acute and chronic infections in humans. During infections, pathogens produce different sets of virulence genes for colonization, tissue damage, and dissemination and for countering host immune responses. Complex regulatory networks control the delicate tuning of gene expression in response to host environments to enable the survival and growth of invading pathogens. Here we identified suhB as a critical gene for the regulation of virulence factors in P. aeruginosa. The expression of suhB was upregulated during acute infection in an animal model, and mutation of suhB rendered P. aeruginosa avirulent. Moreover, we demonstrate that SuhB is required for the activation of virulence factors associated with acute infections while suppressing virulence factors associated with chronic infections. Our report provides new insights into the multilayered regulatory network of virulence genes in P. aeruginosa.Kewei LiChang XuYongxin JinZiyu SunChang LiuJing ShiGukui ChenRonghao ChenShouguang JinWeihui WuAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 6 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Kewei Li Chang Xu Yongxin Jin Ziyu Sun Chang Liu Jing Shi Gukui Chen Ronghao Chen Shouguang Jin Weihui Wu SuhB Is a Regulator of Multiple Virulence Genes and Essential for Pathogenesis of <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
description	ABSTRACT During initial colonization and chronic infection, pathogenic bacteria encounter distinct host environments. Adjusting gene expression accordingly is essential for the pathogenesis. Pseudomonas aeruginosa has evolved complicated regulatory networks to regulate different sets of virulence factors to facilitate colonization and persistence. The type III secretion system (T3SS) and motility are associated with acute infections, while biofilm formation and the type VI secretion system (T6SS) are associated with chronic persistence. To identify novel regulatory genes required for pathogenesis, we screened a P. aeruginosa transposon (Tn) insertion library and found suhB to be an essential gene for the T3SS gene expression. The expression of suhB was upregulated in a mouse acute lung infection model, and loss of suhB resulted in avirulence. Suppression of T3SS gene expression in the suhB mutant is linked to a defective translation of the T3SS master regulator, ExsA. Further studies demonstrated that suhB mutation led to the upregulation of GacA and its downstream small RNAs, RsmY and RsmZ, triggering T6SS expression and biofilm formation while inhibiting the T3SS. Our results demonstrate that an in vivo-inducible gene, suhB, reciprocally regulates genes associated with acute and chronic infections and plays an essential role in the pathogenesis of P. aeruginosa. IMPORTANCE A variety of bacterial pathogens, such as Pseudomonas aeruginosa, cause acute and chronic infections in humans. During infections, pathogens produce different sets of virulence genes for colonization, tissue damage, and dissemination and for countering host immune responses. Complex regulatory networks control the delicate tuning of gene expression in response to host environments to enable the survival and growth of invading pathogens. Here we identified suhB as a critical gene for the regulation of virulence factors in P. aeruginosa. The expression of suhB was upregulated during acute infection in an animal model, and mutation of suhB rendered P. aeruginosa avirulent. Moreover, we demonstrate that SuhB is required for the activation of virulence factors associated with acute infections while suppressing virulence factors associated with chronic infections. Our report provides new insights into the multilayered regulatory network of virulence genes in P. aeruginosa.
format	article
author	Kewei Li Chang Xu Yongxin Jin Ziyu Sun Chang Liu Jing Shi Gukui Chen Ronghao Chen Shouguang Jin Weihui Wu
author_facet	Kewei Li Chang Xu Yongxin Jin Ziyu Sun Chang Liu Jing Shi Gukui Chen Ronghao Chen Shouguang Jin Weihui Wu
author_sort	Kewei Li
title	SuhB Is a Regulator of Multiple Virulence Genes and Essential for Pathogenesis of <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_short	SuhB Is a Regulator of Multiple Virulence Genes and Essential for Pathogenesis of <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full	SuhB Is a Regulator of Multiple Virulence Genes and Essential for Pathogenesis of <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_fullStr	SuhB Is a Regulator of Multiple Virulence Genes and Essential for Pathogenesis of <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full_unstemmed	SuhB Is a Regulator of Multiple Virulence Genes and Essential for Pathogenesis of <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_sort	suhb is a regulator of multiple virulence genes and essential for pathogenesis of <named-content content-type="genus-species">pseudomonas aeruginosa</named-content>
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/a7c2d93515bb4bc0a0af15f10e66689a
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SuhB Is a Regulator of Multiple Virulence Genes and Essential for Pathogenesis of <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

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