Determination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro.

Expression of the calcium-sensing receptor (CaSR) has previously been demonstrated in human circulating monocytes (HCM). The present study was designed to measure CaSR expression in HCM and to examine its potential modulation by pro-inflammatory cytokines, Ca2+, vitamin D sterols in U937 cell line....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Julien Paccou, Cédric Boudot, Aurélien Mary, Said Kamel, Tilman Bernhard Drüeke, Patrice Fardellone, Ziad Massy, Michel Brazier, Romuald Mentaverri
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7c75a2249cd48928739a8363938c343
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a7c75a2249cd48928739a8363938c343
record_format	dspace
spelling	oai:doaj.org-article:a7c75a2249cd48928739a8363938c3432021-11-18T08:52:55ZDetermination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro.1932-620310.1371/journal.pone.0074800https://doaj.org/article/a7c75a2249cd48928739a8363938c3432013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24098349/?tool=EBIhttps://doaj.org/toc/1932-6203Expression of the calcium-sensing receptor (CaSR) has previously been demonstrated in human circulating monocytes (HCM). The present study was designed to measure CaSR expression in HCM and to examine its potential modulation by pro-inflammatory cytokines, Ca2+, vitamin D sterols in U937 cell line. Twenty healthy volunteers underwent blood sampling with subsequent isolation of peripheral blood mononuclear cells (PBMC) at 3 visits. Flow cytometry analysis (FACS) was performed initially (V1) and 19 days later (V2) to examine intra- and intersubject fluctuations of total and surface CaSR expression in HCM and 15 weeks later (V3) to study the effect of vitamin D supplementation. In vitro experiments were conducted to assess the effects of pro-inflammatory cytokines, calcidiol, calcitriol and Ca2+ on CaSR expression in U937 cell line. By FACS analysis, more than 95% of HCM exhibited cell surface CaSR staining. In contrast, CaSR staining failed to detect surface CaSR expression in other PBMC. After cell permeabilization, total CaSR expression was observed in more than 95% of all types of PBMC. Both total and surface CaSR expression in HCM showed a high degree of intra-assay reproducibility (<3%) and a moderate intersubject fluctuation. In response to vitamin D supplementation, there was no significant change for both total and surface CaSR expression. In the in vitro study, U937 cells showed strong total and surface CaSR expression, and both were moderately increased in response to calcitriol exposure. Neither total nor surface CaSR expression was modified by increasing Ca2+ concentrations. Total CaSR expression was concentration dependently decreased by TNFα exposure. In conclusion, CaSR expression can be easily measured by flow cytometry in human circulating monocytes. In the in vitro study, total and surface CaSR expression in the U937 cell line were increased by calcitriol but total CaSR expression was decreased by TNFα stimulation.Julien PaccouCédric BoudotAurélien MarySaid KamelTilman Bernhard DrüekePatrice FardelloneZiad MassyMichel BrazierRomuald MentaverriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e74800 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julien Paccou Cédric Boudot Aurélien Mary Said Kamel Tilman Bernhard Drüeke Patrice Fardellone Ziad Massy Michel Brazier Romuald Mentaverri Determination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro.
description	Expression of the calcium-sensing receptor (CaSR) has previously been demonstrated in human circulating monocytes (HCM). The present study was designed to measure CaSR expression in HCM and to examine its potential modulation by pro-inflammatory cytokines, Ca2+, vitamin D sterols in U937 cell line. Twenty healthy volunteers underwent blood sampling with subsequent isolation of peripheral blood mononuclear cells (PBMC) at 3 visits. Flow cytometry analysis (FACS) was performed initially (V1) and 19 days later (V2) to examine intra- and intersubject fluctuations of total and surface CaSR expression in HCM and 15 weeks later (V3) to study the effect of vitamin D supplementation. In vitro experiments were conducted to assess the effects of pro-inflammatory cytokines, calcidiol, calcitriol and Ca2+ on CaSR expression in U937 cell line. By FACS analysis, more than 95% of HCM exhibited cell surface CaSR staining. In contrast, CaSR staining failed to detect surface CaSR expression in other PBMC. After cell permeabilization, total CaSR expression was observed in more than 95% of all types of PBMC. Both total and surface CaSR expression in HCM showed a high degree of intra-assay reproducibility (<3%) and a moderate intersubject fluctuation. In response to vitamin D supplementation, there was no significant change for both total and surface CaSR expression. In the in vitro study, U937 cells showed strong total and surface CaSR expression, and both were moderately increased in response to calcitriol exposure. Neither total nor surface CaSR expression was modified by increasing Ca2+ concentrations. Total CaSR expression was concentration dependently decreased by TNFα exposure. In conclusion, CaSR expression can be easily measured by flow cytometry in human circulating monocytes. In the in vitro study, total and surface CaSR expression in the U937 cell line were increased by calcitriol but total CaSR expression was decreased by TNFα stimulation.
format	article
author	Julien Paccou Cédric Boudot Aurélien Mary Said Kamel Tilman Bernhard Drüeke Patrice Fardellone Ziad Massy Michel Brazier Romuald Mentaverri
author_facet	Julien Paccou Cédric Boudot Aurélien Mary Said Kamel Tilman Bernhard Drüeke Patrice Fardellone Ziad Massy Michel Brazier Romuald Mentaverri
author_sort	Julien Paccou
title	Determination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro.
title_short	Determination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro.
title_full	Determination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro.
title_fullStr	Determination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro.
title_full_unstemmed	Determination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro.
title_sort	determination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a7c75a2249cd48928739a8363938c343
work_keys_str_mv	AT julienpaccou determinationandmodulationoftotalandsurfacecalciumsensingreceptorexpressioninmonocytesinvivoandinvitro AT cedricboudot determinationandmodulationoftotalandsurfacecalciumsensingreceptorexpressioninmonocytesinvivoandinvitro AT aurelienmary determinationandmodulationoftotalandsurfacecalciumsensingreceptorexpressioninmonocytesinvivoandinvitro AT saidkamel determinationandmodulationoftotalandsurfacecalciumsensingreceptorexpressioninmonocytesinvivoandinvitro AT tilmanbernharddrueke determinationandmodulationoftotalandsurfacecalciumsensingreceptorexpressioninmonocytesinvivoandinvitro AT patricefardellone determinationandmodulationoftotalandsurfacecalciumsensingreceptorexpressioninmonocytesinvivoandinvitro AT ziadmassy determinationandmodulationoftotalandsurfacecalciumsensingreceptorexpressioninmonocytesinvivoandinvitro AT michelbrazier determinationandmodulationoftotalandsurfacecalciumsensingreceptorexpressioninmonocytesinvivoandinvitro AT romualdmentaverri determinationandmodulationoftotalandsurfacecalciumsensingreceptorexpressioninmonocytesinvivoandinvitro
_version_	1718421221191712768

Determination and modulation of total and surface calcium-sensing receptor expression in monocytes in vivo and in vitro.

Ejemplares similares