A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community.
Methods that use homologous recombination to engineer the genome of C. elegans commonly use strains carrying specific insertions of the heterologous transposon Mos1. A large collection of known Mos1 insertion alleles would therefore be of general interest to the C. elegans research community. We des...
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2012
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oai:doaj.org-article:a7c951c602b849bf8f44370beeb4c5b82021-11-18T07:28:43ZA genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community.1932-620310.1371/journal.pone.0030482https://doaj.org/article/a7c951c602b849bf8f44370beeb4c5b82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22347378/?tool=EBIhttps://doaj.org/toc/1932-6203Methods that use homologous recombination to engineer the genome of C. elegans commonly use strains carrying specific insertions of the heterologous transposon Mos1. A large collection of known Mos1 insertion alleles would therefore be of general interest to the C. elegans research community. We describe here the optimization of a semi-automated methodology for the construction of a substantial collection of Mos1 insertion mutant strains. At peak production, more than 5,000 strains were generated per month. These strains were then subject to molecular analysis, and more than 13,300 Mos1 insertions characterized. In addition to targeting directly more than 4,700 genes, these alleles represent the potential starting point for the engineered deletion of essentially all C. elegans genes and the modification of more than 40% of them. This collection of mutants, generated under the auspices of the European NEMAGENETAG consortium, is publicly available and represents an important research resource.Elodie VallinJoseph GallagherLaure GrangerEdwige MartinJérôme BelougneJulien MaurizioYohann DuvergerSarah ScaglioneCaroline BorrelElisabeth CortierKarima AbouzidMaité Carre-PierratKathrin GieselerLaurent SégalatPatricia E KuwabaraJonathan J EwbankPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e30482 (2012) |
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Medicine R Science Q Elodie Vallin Joseph Gallagher Laure Granger Edwige Martin Jérôme Belougne Julien Maurizio Yohann Duverger Sarah Scaglione Caroline Borrel Elisabeth Cortier Karima Abouzid Maité Carre-Pierrat Kathrin Gieseler Laurent Ségalat Patricia E Kuwabara Jonathan J Ewbank A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community. |
| description |
Methods that use homologous recombination to engineer the genome of C. elegans commonly use strains carrying specific insertions of the heterologous transposon Mos1. A large collection of known Mos1 insertion alleles would therefore be of general interest to the C. elegans research community. We describe here the optimization of a semi-automated methodology for the construction of a substantial collection of Mos1 insertion mutant strains. At peak production, more than 5,000 strains were generated per month. These strains were then subject to molecular analysis, and more than 13,300 Mos1 insertions characterized. In addition to targeting directly more than 4,700 genes, these alleles represent the potential starting point for the engineered deletion of essentially all C. elegans genes and the modification of more than 40% of them. This collection of mutants, generated under the auspices of the European NEMAGENETAG consortium, is publicly available and represents an important research resource. |
| format |
article |
| author |
Elodie Vallin Joseph Gallagher Laure Granger Edwige Martin Jérôme Belougne Julien Maurizio Yohann Duverger Sarah Scaglione Caroline Borrel Elisabeth Cortier Karima Abouzid Maité Carre-Pierrat Kathrin Gieseler Laurent Ségalat Patricia E Kuwabara Jonathan J Ewbank |
| author_facet |
Elodie Vallin Joseph Gallagher Laure Granger Edwige Martin Jérôme Belougne Julien Maurizio Yohann Duverger Sarah Scaglione Caroline Borrel Elisabeth Cortier Karima Abouzid Maité Carre-Pierrat Kathrin Gieseler Laurent Ségalat Patricia E Kuwabara Jonathan J Ewbank |
| author_sort |
Elodie Vallin |
| title |
A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community. |
| title_short |
A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community. |
| title_full |
A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community. |
| title_fullStr |
A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community. |
| title_full_unstemmed |
A genome-wide collection of Mos1 transposon insertion mutants for the C. elegans research community. |
| title_sort |
genome-wide collection of mos1 transposon insertion mutants for the c. elegans research community. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/a7c951c602b849bf8f44370beeb4c5b8 |
| work_keys_str_mv |
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