Production of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in Escherichia coli

Abstract Polyphenols, which include phenolic acids, flavonoids, stilbenes, and phenylethanoids, are generally known as useful antioxidants. Tyrosol, hydroxytyrosol, and salidroside are typical phenylethanoids. Phenylethanoids are found in plants such as olive, green tea, and Rhodiola and have variou...

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Autores principales:	Daeun Chung, So Yeon Kim, Joong-Hoon Ahn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7c9aaf699f74315bb9eccc0e3646643
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spelling	oai:doaj.org-article:a7c9aaf699f74315bb9eccc0e36466432021-12-02T16:06:51ZProduction of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in Escherichia coli10.1038/s41598-017-02042-22045-2322https://doaj.org/article/a7c9aaf699f74315bb9eccc0e36466432017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02042-2https://doaj.org/toc/2045-2322Abstract Polyphenols, which include phenolic acids, flavonoids, stilbenes, and phenylethanoids, are generally known as useful antioxidants. Tyrosol, hydroxytyrosol, and salidroside are typical phenylethanoids. Phenylethanoids are found in plants such as olive, green tea, and Rhodiola and have various biological activities, including the prevention of cardiovascular diseases, cancer, and brain damage. We used Escherichia coli to synthesize three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside. To synthesize tyrosol, the aromatic aldehyde synthase (AAS) was expressed in E. coli. Hydroxytyrosol was synthesized using E. coli harboring AAS and HpaBC, which encodes hydroxylase. In order to synthesize salidroside, 12 uridine diphosphate-dependent glycosyltransferases (UGTs) were screened and UGT85A1 was found to convert tyrosol to salidroside. Using E. coli harboring AAS and UGT85A1, salidroside was synthesized. Through the optimization of these three E. coli strains, we were able to synthesize 531 mg/L tyrosol, 208 mg/L hydroxytyrosol, and 288 mg/L salidroside, respectively.Daeun ChungSo Yeon KimJoong-Hoon AhnNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daeun Chung So Yeon Kim Joong-Hoon Ahn Production of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in Escherichia coli
description	Abstract Polyphenols, which include phenolic acids, flavonoids, stilbenes, and phenylethanoids, are generally known as useful antioxidants. Tyrosol, hydroxytyrosol, and salidroside are typical phenylethanoids. Phenylethanoids are found in plants such as olive, green tea, and Rhodiola and have various biological activities, including the prevention of cardiovascular diseases, cancer, and brain damage. We used Escherichia coli to synthesize three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside. To synthesize tyrosol, the aromatic aldehyde synthase (AAS) was expressed in E. coli. Hydroxytyrosol was synthesized using E. coli harboring AAS and HpaBC, which encodes hydroxylase. In order to synthesize salidroside, 12 uridine diphosphate-dependent glycosyltransferases (UGTs) were screened and UGT85A1 was found to convert tyrosol to salidroside. Using E. coli harboring AAS and UGT85A1, salidroside was synthesized. Through the optimization of these three E. coli strains, we were able to synthesize 531 mg/L tyrosol, 208 mg/L hydroxytyrosol, and 288 mg/L salidroside, respectively.
format	article
author	Daeun Chung So Yeon Kim Joong-Hoon Ahn
author_facet	Daeun Chung So Yeon Kim Joong-Hoon Ahn
author_sort	Daeun Chung
title	Production of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in Escherichia coli
title_short	Production of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in Escherichia coli
title_full	Production of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in Escherichia coli
title_fullStr	Production of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in Escherichia coli
title_full_unstemmed	Production of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in Escherichia coli
title_sort	production of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in escherichia coli
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a7c9aaf699f74315bb9eccc0e3646643
work_keys_str_mv	AT daeunchung productionofthreephenylethanoidstyrosolhydroxytyrosolandsalidrosideusingplantgenesexpressinginescherichiacoli AT soyeonkim productionofthreephenylethanoidstyrosolhydroxytyrosolandsalidrosideusingplantgenesexpressinginescherichiacoli AT joonghoonahn productionofthreephenylethanoidstyrosolhydroxytyrosolandsalidrosideusingplantgenesexpressinginescherichiacoli
_version_	1718384861306159104

Production of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in Escherichia coli

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