Characterization of Flavonoids and Transcripts Involved in Their Biosynthesis in Different Organs of Cissus rotundifolia Lam

Cissus rotundifolia Lam. is used as a medicinal herb and vegetable. Flavonoids are the major components for the therapeutic effects. However, flavonoids constituents and expression profiles of related genes in C. rotundifolia organs are unknown. Colorimetric ass...

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Autores principales:	Duncan Kiragu Gichuki, Qingyun Li, Yujun Hou, Yuanshuang Liu, Mengxue Ma, Huimin Zhou, Chen Xu, Zhenfei Zhu, Lina Wang, Fredrick Mutie Musila, Qingfeng Wang, Haiping Xin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Cissus rotundifolia</i> flavonoids metabolites biosynthesis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a7caf324178542f48699e1f4e7c0b0fa
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spelling	oai:doaj.org-article:a7caf324178542f48699e1f4e7c0b0fa2021-11-25T18:20:35ZCharacterization of Flavonoids and Transcripts Involved in Their Biosynthesis in Different Organs of <i>Cissus rotundifolia</i> Lam10.3390/metabo111107412218-1989https://doaj.org/article/a7caf324178542f48699e1f4e7c0b0fa2021-10-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/741https://doaj.org/toc/2218-1989<i>Cissus rotundifolia</i> Lam. is used as a medicinal herb and vegetable. Flavonoids are the major components for the therapeutic effects. However, flavonoids constituents and expression profiles of related genes in <i>C. rotundifolia</i> organs are unknown. Colorimetric assay showed the highest flavonoid concentration in roots compared to the stem and leaf. Widely target-based metabolome analysis allowed tentative identification of 199 compounds in three organs. Flavonols and flavones were the dominant flavonoids subclasses. Among the metabolites, 171 were common in the three organs. Unique accumulation profile was observed in the root while the stem and leaf exhibited relatively similar patterns. In the root, six unique compounds (jaceosidin, licoagrochalcone D, 8-prenylkaempferol, hesperetin 7-O-(6″malonyl) glucoside, aureusidin, apigenin-4′-O-rhamnoside) that are used for medicinal purposes were detected. In total, 18,427 expressed genes were identified from transcriptome of the three organs covering about 60% of annotated genes in <i>C. rotundifolia</i> genome. Fourteen gene families, including 52 members involved in the main pathway of flavonoids biosynthesis, were identified. Their expression could be found in at least one organ. Most of the genes were highly expressed in roots compared to other organs, coinciding with the metabolites profile. The findings provide fundamental data for exploration of metabolites biosynthesis in <i>C. rotundifolia</i> and diversification of parts used for medicinal purposes.Duncan Kiragu GichukiQingyun LiYujun HouYuanshuang LiuMengxue MaHuimin ZhouChen XuZhenfei ZhuLina WangFredrick Mutie MusilaQingfeng WangHaiping XinMDPI AGarticle<i>Cissus rotundifolia</i>flavonoidsmetabolitesbiosynthesisMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 741, p 741 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Cissus rotundifolia</i> flavonoids metabolites biosynthesis Microbiology QR1-502
spellingShingle	<i>Cissus rotundifolia</i> flavonoids metabolites biosynthesis Microbiology QR1-502 Duncan Kiragu Gichuki Qingyun Li Yujun Hou Yuanshuang Liu Mengxue Ma Huimin Zhou Chen Xu Zhenfei Zhu Lina Wang Fredrick Mutie Musila Qingfeng Wang Haiping Xin Characterization of Flavonoids and Transcripts Involved in Their Biosynthesis in Different Organs of <i>Cissus rotundifolia</i> Lam
description	<i>Cissus rotundifolia</i> Lam. is used as a medicinal herb and vegetable. Flavonoids are the major components for the therapeutic effects. However, flavonoids constituents and expression profiles of related genes in <i>C. rotundifolia</i> organs are unknown. Colorimetric assay showed the highest flavonoid concentration in roots compared to the stem and leaf. Widely target-based metabolome analysis allowed tentative identification of 199 compounds in three organs. Flavonols and flavones were the dominant flavonoids subclasses. Among the metabolites, 171 were common in the three organs. Unique accumulation profile was observed in the root while the stem and leaf exhibited relatively similar patterns. In the root, six unique compounds (jaceosidin, licoagrochalcone D, 8-prenylkaempferol, hesperetin 7-O-(6″malonyl) glucoside, aureusidin, apigenin-4′-O-rhamnoside) that are used for medicinal purposes were detected. In total, 18,427 expressed genes were identified from transcriptome of the three organs covering about 60% of annotated genes in <i>C. rotundifolia</i> genome. Fourteen gene families, including 52 members involved in the main pathway of flavonoids biosynthesis, were identified. Their expression could be found in at least one organ. Most of the genes were highly expressed in roots compared to other organs, coinciding with the metabolites profile. The findings provide fundamental data for exploration of metabolites biosynthesis in <i>C. rotundifolia</i> and diversification of parts used for medicinal purposes.
format	article
author	Duncan Kiragu Gichuki Qingyun Li Yujun Hou Yuanshuang Liu Mengxue Ma Huimin Zhou Chen Xu Zhenfei Zhu Lina Wang Fredrick Mutie Musila Qingfeng Wang Haiping Xin
author_facet	Duncan Kiragu Gichuki Qingyun Li Yujun Hou Yuanshuang Liu Mengxue Ma Huimin Zhou Chen Xu Zhenfei Zhu Lina Wang Fredrick Mutie Musila Qingfeng Wang Haiping Xin
author_sort	Duncan Kiragu Gichuki
title	Characterization of Flavonoids and Transcripts Involved in Their Biosynthesis in Different Organs of <i>Cissus rotundifolia</i> Lam
title_short	Characterization of Flavonoids and Transcripts Involved in Their Biosynthesis in Different Organs of <i>Cissus rotundifolia</i> Lam
title_full	Characterization of Flavonoids and Transcripts Involved in Their Biosynthesis in Different Organs of <i>Cissus rotundifolia</i> Lam
title_fullStr	Characterization of Flavonoids and Transcripts Involved in Their Biosynthesis in Different Organs of <i>Cissus rotundifolia</i> Lam
title_full_unstemmed	Characterization of Flavonoids and Transcripts Involved in Their Biosynthesis in Different Organs of <i>Cissus rotundifolia</i> Lam
title_sort	characterization of flavonoids and transcripts involved in their biosynthesis in different organs of <i>cissus rotundifolia</i> lam
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a7caf324178542f48699e1f4e7c0b0fa
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Characterization of Flavonoids and Transcripts Involved in Their Biosynthesis in Different Organs of <i>Cissus rotundifolia</i> Lam

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