Automated multiplex genome-scale engineering in yeast

Genome-scale engineering is a powerful technique for understanding biology and designing microorganisms but has been limited to bacterial species. Here the authors present an automated platform for genome-scale engineering inSaccharomyces cerevisiaeusing CRISPR-Cas and RNAi.

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Detalles Bibliográficos
Autores principales: Tong Si, Ran Chao, Yuhao Min, Yuying Wu, Wen Ren, Huimin Zhao
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/a7ce9e59cfef44bc9ab5ef2744240359
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Descripción
Sumario:Genome-scale engineering is a powerful technique for understanding biology and designing microorganisms but has been limited to bacterial species. Here the authors present an automated platform for genome-scale engineering inSaccharomyces cerevisiaeusing CRISPR-Cas and RNAi.