Circulating monocytes and B-lymphocytes in neovascular age-related macular degeneration

Sven Magnus Hector,1 Torben Lykke Sørensen1,2 1Clinical Eye Research Unit, Zealand University Hospital, Roskilde, 2Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark Background: Individuals with neovascular age-related macular degeneration (AMD) have a...

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Autores principales:	Hector SM, Sørensen TL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Macular degeneration Choroidal Neovascularisation Monocytes B-Lymphocytes Flow Cytometry Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/a7d10b6013b34c59be8411a924746f0e
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spelling	oai:doaj.org-article:a7d10b6013b34c59be8411a924746f0e2021-12-02T04:43:28ZCirculating monocytes and B-lymphocytes in neovascular age-related macular degeneration1177-5483https://doaj.org/article/a7d10b6013b34c59be8411a924746f0e2017-01-01T00:00:00Zhttps://www.dovepress.com/circulating-monocytes-and-b-lymphocytes-in-neovascular-age-related-mac-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Sven Magnus Hector,1 Torben Lykke Sørensen1,2 1Clinical Eye Research Unit, Zealand University Hospital, Roskilde, 2Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark Background: Individuals with neovascular age-related macular degeneration (AMD) have altered number and distribution of retinal macrophages and show changes in circulating antibodies. We wanted to investigate the corresponding precursors, with subpopulations. We therefore measured monocyte and B-lymphocyte populations in individuals with neovascular AMD.Design: This was an observational case–control study.Participants or samples: A total of 31 individuals with neovascular AMD and 30 healthy age-matched controls were included.Methods: Patients and controls were interviewed, and ophthalmological examination included visual acuity assessment using the Early Treatment Diabetic Retinopathy Study (ETDRS) chart, spectral domain optical coherence tomography (SD-OCT), slit-lamp examination and fundus photography. Moreover, venous blood was drawn and prepared for flow cytometry. Cells were gated and measured for surface markers.Main outcome measures: Relative amounts of monocytes and B-lymphocytes with subsets, as well as selected surface markers, were measured.Results: The two groups did not significantly differ in age, smoking history, body mass index, physical activity or C-reactive protein (CRP). Total monocytes (percentage of all leukocytes) were lower in the neovascular AMD group (median 5.5%) compared with the level in the control group (6.5%; P-value: 0.028). The percentage of intermediate monocytes positive for cluster of differentiation 11b (CD11b) was lower for AMD patients (99.4%) compared with 100% for the control group (P-value: 0.032).Conclusion: We observed lower numbers of monocytes, which show a potentially impaired ability to migrate across the endothelial wall in patients with neovascular AMD. These subtle changes could potentially lead to an imbalance in the recruitment of macrophages into the retina during disease development. Keywords: macular degeneration, choroidal neovascularization, flow cytometryHector SMSørensen TLDove Medical PressarticleMacular degenerationChoroidal NeovascularisationMonocytesB-LymphocytesFlow CytometryOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 11, Pp 179-184 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Macular degeneration Choroidal Neovascularisation Monocytes B-Lymphocytes Flow Cytometry Ophthalmology RE1-994
spellingShingle	Macular degeneration Choroidal Neovascularisation Monocytes B-Lymphocytes Flow Cytometry Ophthalmology RE1-994 Hector SM Sørensen TL Circulating monocytes and B-lymphocytes in neovascular age-related macular degeneration
description	Sven Magnus Hector,1 Torben Lykke Sørensen1,2 1Clinical Eye Research Unit, Zealand University Hospital, Roskilde, 2Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark Background: Individuals with neovascular age-related macular degeneration (AMD) have altered number and distribution of retinal macrophages and show changes in circulating antibodies. We wanted to investigate the corresponding precursors, with subpopulations. We therefore measured monocyte and B-lymphocyte populations in individuals with neovascular AMD.Design: This was an observational case–control study.Participants or samples: A total of 31 individuals with neovascular AMD and 30 healthy age-matched controls were included.Methods: Patients and controls were interviewed, and ophthalmological examination included visual acuity assessment using the Early Treatment Diabetic Retinopathy Study (ETDRS) chart, spectral domain optical coherence tomography (SD-OCT), slit-lamp examination and fundus photography. Moreover, venous blood was drawn and prepared for flow cytometry. Cells were gated and measured for surface markers.Main outcome measures: Relative amounts of monocytes and B-lymphocytes with subsets, as well as selected surface markers, were measured.Results: The two groups did not significantly differ in age, smoking history, body mass index, physical activity or C-reactive protein (CRP). Total monocytes (percentage of all leukocytes) were lower in the neovascular AMD group (median 5.5%) compared with the level in the control group (6.5%; P-value: 0.028). The percentage of intermediate monocytes positive for cluster of differentiation 11b (CD11b) was lower for AMD patients (99.4%) compared with 100% for the control group (P-value: 0.032).Conclusion: We observed lower numbers of monocytes, which show a potentially impaired ability to migrate across the endothelial wall in patients with neovascular AMD. These subtle changes could potentially lead to an imbalance in the recruitment of macrophages into the retina during disease development. Keywords: macular degeneration, choroidal neovascularization, flow cytometry
format	article
author	Hector SM Sørensen TL
author_facet	Hector SM Sørensen TL
author_sort	Hector SM
title	Circulating monocytes and B-lymphocytes in neovascular age-related macular degeneration
title_short	Circulating monocytes and B-lymphocytes in neovascular age-related macular degeneration
title_full	Circulating monocytes and B-lymphocytes in neovascular age-related macular degeneration
title_fullStr	Circulating monocytes and B-lymphocytes in neovascular age-related macular degeneration
title_full_unstemmed	Circulating monocytes and B-lymphocytes in neovascular age-related macular degeneration
title_sort	circulating monocytes and b-lymphocytes in neovascular age-related macular degeneration
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/a7d10b6013b34c59be8411a924746f0e
work_keys_str_mv	AT hectorsm circulatingmonocytesandblymphocytesinneovascularagerelatedmaculardegeneration AT sørensentl circulatingmonocytesandblymphocytesinneovascularagerelatedmaculardegeneration
_version_	1718401096042414080

Circulating monocytes and B-lymphocytes in neovascular age-related macular degeneration

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