Positional information is reprogrammed in blastema cells of the regenerating limb of the axolotl (Ambystoma mexicanum).

The regenerating region of an amputated salamander limb, known as the blastema, has the amazing capacity to replace exactly the missing structures. By grafting cells from different stages and regions of blastemas induced to form on donor animals expressing Green Fluorescent Protein (GFP), to non-GFP...

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Autores principales:	Catherine D McCusker, David M Gardiner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a7de080a332d4864b1df406ccd8ce020
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spelling	oai:doaj.org-article:a7de080a332d4864b1df406ccd8ce0202021-11-18T08:53:33ZPositional information is reprogrammed in blastema cells of the regenerating limb of the axolotl (Ambystoma mexicanum).1932-620310.1371/journal.pone.0077064https://doaj.org/article/a7de080a332d4864b1df406ccd8ce0202013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24086768/?tool=EBIhttps://doaj.org/toc/1932-6203The regenerating region of an amputated salamander limb, known as the blastema, has the amazing capacity to replace exactly the missing structures. By grafting cells from different stages and regions of blastemas induced to form on donor animals expressing Green Fluorescent Protein (GFP), to non-GFP host animals, we have determined that the cells from early stage blastemas, as well as cells at the tip of late stage blastemas are developmentally labile such that their positional identity is reprogrammed by interactions with more proximal cells with stable positional information. In contrast, cells from the adjacent, more proximal stump tissues as well as the basal region of late bud blastemas are positionally stable, and thus form ectopic limb structures when grafted. Finally, we have found that a nerve is required to maintain the blastema cells in a positionally labile state, thus indicating a role for reprogramming cues in the blastema microenvironment.Catherine D McCuskerDavid M GardinerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e77064 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Catherine D McCusker David M Gardiner Positional information is reprogrammed in blastema cells of the regenerating limb of the axolotl (Ambystoma mexicanum).
description	The regenerating region of an amputated salamander limb, known as the blastema, has the amazing capacity to replace exactly the missing structures. By grafting cells from different stages and regions of blastemas induced to form on donor animals expressing Green Fluorescent Protein (GFP), to non-GFP host animals, we have determined that the cells from early stage blastemas, as well as cells at the tip of late stage blastemas are developmentally labile such that their positional identity is reprogrammed by interactions with more proximal cells with stable positional information. In contrast, cells from the adjacent, more proximal stump tissues as well as the basal region of late bud blastemas are positionally stable, and thus form ectopic limb structures when grafted. Finally, we have found that a nerve is required to maintain the blastema cells in a positionally labile state, thus indicating a role for reprogramming cues in the blastema microenvironment.
format	article
author	Catherine D McCusker David M Gardiner
author_facet	Catherine D McCusker David M Gardiner
author_sort	Catherine D McCusker
title	Positional information is reprogrammed in blastema cells of the regenerating limb of the axolotl (Ambystoma mexicanum).
title_short	Positional information is reprogrammed in blastema cells of the regenerating limb of the axolotl (Ambystoma mexicanum).
title_full	Positional information is reprogrammed in blastema cells of the regenerating limb of the axolotl (Ambystoma mexicanum).
title_fullStr	Positional information is reprogrammed in blastema cells of the regenerating limb of the axolotl (Ambystoma mexicanum).
title_full_unstemmed	Positional information is reprogrammed in blastema cells of the regenerating limb of the axolotl (Ambystoma mexicanum).
title_sort	positional information is reprogrammed in blastema cells of the regenerating limb of the axolotl (ambystoma mexicanum).
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a7de080a332d4864b1df406ccd8ce020
work_keys_str_mv	AT catherinedmccusker positionalinformationisreprogrammedinblastemacellsoftheregeneratinglimboftheaxolotlambystomamexicanum AT davidmgardiner positionalinformationisreprogrammedinblastemacellsoftheregeneratinglimboftheaxolotlambystomamexicanum
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Positional information is reprogrammed in blastema cells of the regenerating limb of the axolotl (Ambystoma mexicanum).

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