Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes

ABSTRACT Plant roots are usually colonized by various arbuscular mycorrhizal (AM) fungal species, which vary in morphological, physiological, and genetic traits. This colonization constitutes the mycorrhizal nutrient uptake pathway (MP) and supplements the pathway through roots. Simultaneously, the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiachao Zhou, Xiaofen Chai, Lin Zhang, Timothy S. George, Fei Wang, Gu Feng
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	13C-DNA-SIP arbuscular mycorrhizae COG hyphal exudates hyphosphere microbiome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a7df4fb9bdb84f29af6b0d10255514c6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a7df4fb9bdb84f29af6b0d10255514c6
record_format	dspace
spelling	oai:doaj.org-article:a7df4fb9bdb84f29af6b0d10255514c62021-12-02T19:46:19ZDifferent Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes10.1128/mSystems.00929-202379-5077https://doaj.org/article/a7df4fb9bdb84f29af6b0d10255514c62020-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00929-20https://doaj.org/toc/2379-5077ABSTRACT Plant roots are usually colonized by various arbuscular mycorrhizal (AM) fungal species, which vary in morphological, physiological, and genetic traits. This colonization constitutes the mycorrhizal nutrient uptake pathway (MP) and supplements the pathway through roots. Simultaneously, the extraradical hyphae of each AM fungus is associated with a community of bacteria. However, whether the community structure and function of the microbiome on the extraradical hyphae differ between AM fungal species remains unknown. In order to understand the community structure and the predicted functions of the microbiome associated with different AM fungal species, a split-root compartmented rhizobox cultivation system, which allowed us to inoculate two AM fungal species separately in two root compartments, was used. We inoculated two separate AM fungal species combinations, (i) Funneliformis mosseae and Gigaspora margarita and (ii) Rhizophagus intraradices and G. margarita, on a single root system of cotton. The hyphal exudate-fed, active microbiome was measured by combining 13C-DNA stable isotope probing with MiSeq sequencing. We found that different AM fungal species, which were simultaneously colonizing a single root system, hosted active microbiomes that were distinct from one another. Moreover, the predicted potential functions of the different microbiomes were distinct. We conclude that the arbuscular mycorrhizal fungal component of the system is responsible for the recruitment of distinct microbiomes in the hyphosphere. The potential significance of the predicted functions of the microbial ecosystem services is discussed. IMPORTANCE Arbuscular mycorrhizal (AM) fungi form tight symbiotic relationships with the majority of terrestrial plants and play critical roles in plant P acquisition, adding a further dimension of complexity. The plant-AM fungus-bacterium system is considered a continuum, with the bacteria colonizing not only the plant roots, but also the associated mycorrhizal hyphal network, known as the hyphosphere microbiome. Plant roots are usually colonized by different AM fungal species which form an independent phosphorus uptake pathway from the root pathway, i.e., the mycorrhizal pathway. The community structure and function of the hyphosphere microbiome of different AM species are completely unknown. In this novel study, we found that arbuscular mycorrhizal fungi cocolonizing on single plant roots recruit their own specific microbiomes, which should be considered in evaluating plant microbiome form and function. Our findings demonstrate the importance of understanding trophic interactions in order to gain insight into the plant-AM fungus-bacterium symbiosis.Jiachao ZhouXiaofen ChaiLin ZhangTimothy S. GeorgeFei WangGu FengAmerican Society for Microbiologyarticle13C-DNA-SIParbuscular mycorrhizaeCOGhyphal exudateshyphospheremicrobiomeMicrobiologyQR1-502ENmSystems, Vol 5, Iss 6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	13C-DNA-SIP arbuscular mycorrhizae COG hyphal exudates hyphosphere microbiome Microbiology QR1-502
spellingShingle	13C-DNA-SIP arbuscular mycorrhizae COG hyphal exudates hyphosphere microbiome Microbiology QR1-502 Jiachao Zhou Xiaofen Chai Lin Zhang Timothy S. George Fei Wang Gu Feng Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes
description	ABSTRACT Plant roots are usually colonized by various arbuscular mycorrhizal (AM) fungal species, which vary in morphological, physiological, and genetic traits. This colonization constitutes the mycorrhizal nutrient uptake pathway (MP) and supplements the pathway through roots. Simultaneously, the extraradical hyphae of each AM fungus is associated with a community of bacteria. However, whether the community structure and function of the microbiome on the extraradical hyphae differ between AM fungal species remains unknown. In order to understand the community structure and the predicted functions of the microbiome associated with different AM fungal species, a split-root compartmented rhizobox cultivation system, which allowed us to inoculate two AM fungal species separately in two root compartments, was used. We inoculated two separate AM fungal species combinations, (i) Funneliformis mosseae and Gigaspora margarita and (ii) Rhizophagus intraradices and G. margarita, on a single root system of cotton. The hyphal exudate-fed, active microbiome was measured by combining 13C-DNA stable isotope probing with MiSeq sequencing. We found that different AM fungal species, which were simultaneously colonizing a single root system, hosted active microbiomes that were distinct from one another. Moreover, the predicted potential functions of the different microbiomes were distinct. We conclude that the arbuscular mycorrhizal fungal component of the system is responsible for the recruitment of distinct microbiomes in the hyphosphere. The potential significance of the predicted functions of the microbial ecosystem services is discussed. IMPORTANCE Arbuscular mycorrhizal (AM) fungi form tight symbiotic relationships with the majority of terrestrial plants and play critical roles in plant P acquisition, adding a further dimension of complexity. The plant-AM fungus-bacterium system is considered a continuum, with the bacteria colonizing not only the plant roots, but also the associated mycorrhizal hyphal network, known as the hyphosphere microbiome. Plant roots are usually colonized by different AM fungal species which form an independent phosphorus uptake pathway from the root pathway, i.e., the mycorrhizal pathway. The community structure and function of the hyphosphere microbiome of different AM species are completely unknown. In this novel study, we found that arbuscular mycorrhizal fungi cocolonizing on single plant roots recruit their own specific microbiomes, which should be considered in evaluating plant microbiome form and function. Our findings demonstrate the importance of understanding trophic interactions in order to gain insight into the plant-AM fungus-bacterium symbiosis.
format	article
author	Jiachao Zhou Xiaofen Chai Lin Zhang Timothy S. George Fei Wang Gu Feng
author_facet	Jiachao Zhou Xiaofen Chai Lin Zhang Timothy S. George Fei Wang Gu Feng
author_sort	Jiachao Zhou
title	Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes
title_short	Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes
title_full	Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes
title_fullStr	Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes
title_full_unstemmed	Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes
title_sort	different arbuscular mycorrhizal fungi cocolonizing on a single plant root system recruit distinct microbiomes
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/a7df4fb9bdb84f29af6b0d10255514c6
work_keys_str_mv	AT jiachaozhou differentarbuscularmycorrhizalfungicocolonizingonasingleplantrootsystemrecruitdistinctmicrobiomes AT xiaofenchai differentarbuscularmycorrhizalfungicocolonizingonasingleplantrootsystemrecruitdistinctmicrobiomes AT linzhang differentarbuscularmycorrhizalfungicocolonizingonasingleplantrootsystemrecruitdistinctmicrobiomes AT timothysgeorge differentarbuscularmycorrhizalfungicocolonizingonasingleplantrootsystemrecruitdistinctmicrobiomes AT feiwang differentarbuscularmycorrhizalfungicocolonizingonasingleplantrootsystemrecruitdistinctmicrobiomes AT gufeng differentarbuscularmycorrhizalfungicocolonizingonasingleplantrootsystemrecruitdistinctmicrobiomes
_version_	1718376044578209792

Different Arbuscular Mycorrhizal Fungi Cocolonizing on a Single Plant Root System Recruit Distinct Microbiomes

Ejemplares similares