The mobilization and recruitment of c-kit+ cells contribute to wound healing after surgery.
Delayed wound healing is a serious clinical problem in patients after surgery. A recent study has demonstrated that bone marrow-derived c-kit-positive (c-kit(+)) cells play important roles in repairing and regenerating various tissues and organs. To examine the hypothesis that surgical injury induce...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/a7e46c66a99d41cd9e98afaa41b5a8e9 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:a7e46c66a99d41cd9e98afaa41b5a8e9 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:a7e46c66a99d41cd9e98afaa41b5a8e92021-11-18T08:08:56ZThe mobilization and recruitment of c-kit+ cells contribute to wound healing after surgery.1932-620310.1371/journal.pone.0048052https://doaj.org/article/a7e46c66a99d41cd9e98afaa41b5a8e92012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23155375/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Delayed wound healing is a serious clinical problem in patients after surgery. A recent study has demonstrated that bone marrow-derived c-kit-positive (c-kit(+)) cells play important roles in repairing and regenerating various tissues and organs. To examine the hypothesis that surgical injury induces the mobilization and recruitment of c-kit+ cells to accelerate wound healing. Mice were subjected to a left pneumonectomy. The mobilization of c-kit+ cells was monitored after surgery. Using green fluorescent protein (GFP(+)) bone marrow-transplanted chimera mice, we investigated further whether the mobilized c-kit+ cells were recruited to effect wound healing in a skin puncture model. The group with left pneumonectomies increased the c-kit(+) and CD34(+) stem cells in peripheral blood 24 h after surgery. At 3 days after surgery, the skin wound size was observed to be significantly smaller, and the number of bone marrow-derived GFP(+) cells and GFP(+)/c-kit+ cells in the wound tissue was significantly greater in mice that had received pneumonectomies, as compared with those that had received a sham operation. Furthermore, some of these GFP(+) cells were positively expressed specific markers of macrophages (F4/80), endothelial cells (CD31), and myofibroblasts (αSMA). The administration of AMD3100, an antagonist of a stromal-cell derived factor (SDF)-1/CXCR4 signaling pathway, reduced the number of GFP(+) cells in wound tissue and completely negated the accelerated wound healing. Surgical injury induces the mobilization and recruitment of c-kit+ cells to contribute to wound healing. Regulating c-kit+ cells may provide a new approach that accelerates wound healing after surgery.Yoshihiro TakemotoTao-Sheng LiMasayuki KuboMako OhshimaHiroshi KurazumiKazuhiro UedaTadahiko EnokiTomoaki MurataKimikazu HamanoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48052 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Yoshihiro Takemoto Tao-Sheng Li Masayuki Kubo Mako Ohshima Hiroshi Kurazumi Kazuhiro Ueda Tadahiko Enoki Tomoaki Murata Kimikazu Hamano The mobilization and recruitment of c-kit+ cells contribute to wound healing after surgery. |
| description |
Delayed wound healing is a serious clinical problem in patients after surgery. A recent study has demonstrated that bone marrow-derived c-kit-positive (c-kit(+)) cells play important roles in repairing and regenerating various tissues and organs. To examine the hypothesis that surgical injury induces the mobilization and recruitment of c-kit+ cells to accelerate wound healing. Mice were subjected to a left pneumonectomy. The mobilization of c-kit+ cells was monitored after surgery. Using green fluorescent protein (GFP(+)) bone marrow-transplanted chimera mice, we investigated further whether the mobilized c-kit+ cells were recruited to effect wound healing in a skin puncture model. The group with left pneumonectomies increased the c-kit(+) and CD34(+) stem cells in peripheral blood 24 h after surgery. At 3 days after surgery, the skin wound size was observed to be significantly smaller, and the number of bone marrow-derived GFP(+) cells and GFP(+)/c-kit+ cells in the wound tissue was significantly greater in mice that had received pneumonectomies, as compared with those that had received a sham operation. Furthermore, some of these GFP(+) cells were positively expressed specific markers of macrophages (F4/80), endothelial cells (CD31), and myofibroblasts (αSMA). The administration of AMD3100, an antagonist of a stromal-cell derived factor (SDF)-1/CXCR4 signaling pathway, reduced the number of GFP(+) cells in wound tissue and completely negated the accelerated wound healing. Surgical injury induces the mobilization and recruitment of c-kit+ cells to contribute to wound healing. Regulating c-kit+ cells may provide a new approach that accelerates wound healing after surgery. |
| format |
article |
| author |
Yoshihiro Takemoto Tao-Sheng Li Masayuki Kubo Mako Ohshima Hiroshi Kurazumi Kazuhiro Ueda Tadahiko Enoki Tomoaki Murata Kimikazu Hamano |
| author_facet |
Yoshihiro Takemoto Tao-Sheng Li Masayuki Kubo Mako Ohshima Hiroshi Kurazumi Kazuhiro Ueda Tadahiko Enoki Tomoaki Murata Kimikazu Hamano |
| author_sort |
Yoshihiro Takemoto |
| title |
The mobilization and recruitment of c-kit+ cells contribute to wound healing after surgery. |
| title_short |
The mobilization and recruitment of c-kit+ cells contribute to wound healing after surgery. |
| title_full |
The mobilization and recruitment of c-kit+ cells contribute to wound healing after surgery. |
| title_fullStr |
The mobilization and recruitment of c-kit+ cells contribute to wound healing after surgery. |
| title_full_unstemmed |
The mobilization and recruitment of c-kit+ cells contribute to wound healing after surgery. |
| title_sort |
mobilization and recruitment of c-kit+ cells contribute to wound healing after surgery. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/a7e46c66a99d41cd9e98afaa41b5a8e9 |
| work_keys_str_mv |
AT yoshihirotakemoto themobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT taoshengli themobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT masayukikubo themobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT makoohshima themobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT hiroshikurazumi themobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT kazuhiroueda themobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT tadahikoenoki themobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT tomoakimurata themobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT kimikazuhamano themobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT yoshihirotakemoto mobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT taoshengli mobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT masayukikubo mobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT makoohshima mobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT hiroshikurazumi mobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT kazuhiroueda mobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT tadahikoenoki mobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT tomoakimurata mobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery AT kimikazuhamano mobilizationandrecruitmentofckitcellscontributetowoundhealingaftersurgery |
| _version_ |
1718422186134339584 |