Cytotoxicity and reactive oxygen species generation from aggregated carbon and carbonaceous nanoparticulate materials

Kristine M Garza1, Karla F Soto2, Lawrence E Murr31Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, USA; 2Lockheed Martin Aeronautics Company, Forth Worth, TX, USA; 3Department of Metallurgical and Materials Engineering, The University of Texas at El Paso, El Paso,...

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Autores principales:	Kristine M Garza, Karla F Soto, Lawrence E Murr
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a7f270e01cd141959fbb20e21806ed90
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spelling	oai:doaj.org-article:a7f270e01cd141959fbb20e21806ed902021-12-02T02:10:26ZCytotoxicity and reactive oxygen species generation from aggregated carbon and carbonaceous nanoparticulate materials1176-91141178-2013https://doaj.org/article/a7f270e01cd141959fbb20e21806ed902008-03-01T00:00:00Zhttp://www.dovepress.com/cytotoxicity-and-reactive-oxygen-species-generation-from-aggregated-ca-a740https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Kristine M Garza1, Karla F Soto2, Lawrence E Murr31Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, USA; 2Lockheed Martin Aeronautics Company, Forth Worth, TX, USA; 3Department of Metallurgical and Materials Engineering, The University of Texas at El Paso, El Paso, TX, USAAbstract: We have investigated the cytotoxicity and reactive oxygen species (ROS) generation for indoor and outdoor soots: candle, wood, diesel, tire, and natural gas burner soots &ndash; along with surrogate black carbon, various multiwall carbon nanotube aggregate materials, TiO2 (anatase) and chrysotile asbestos as reference materials. All soots were observed utilizing TEM and FESEM to be composed of aggregated, primary spherules (20&ndash;80 nm diameter) forming complex, branched fractal structures. These spherules were composed of intercalated, turbostratic arrangements of curved graphene fragments with varying concentrations of polycyclic aromatic hydrocarbon (PAH) isomers. In vitro cultures with an immortalized human lung epithelial carcinoma cell line (A549) treated with these materials showed decreased cell viability and variations in ROS production, with no correlations to PAH content. The data demonstrate that soots are cytotoxic and that cytotoxicity is not related to PAH content but is related to ROS generation, suggesting that soot induces cellular oxidative stress and that cell viability assays can be indicators of ROS production.Keywords: cytotoxicity assessment, ROS assays, FESEM and TEM analysis, nanoparticulate aggregates Kristine M GarzaKarla F SotoLawrence E MurrDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2008, Iss Issue 1, Pp 83-94 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kristine M Garza Karla F Soto Lawrence E Murr Cytotoxicity and reactive oxygen species generation from aggregated carbon and carbonaceous nanoparticulate materials
description	Kristine M Garza1, Karla F Soto2, Lawrence E Murr31Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, USA; 2Lockheed Martin Aeronautics Company, Forth Worth, TX, USA; 3Department of Metallurgical and Materials Engineering, The University of Texas at El Paso, El Paso, TX, USAAbstract: We have investigated the cytotoxicity and reactive oxygen species (ROS) generation for indoor and outdoor soots: candle, wood, diesel, tire, and natural gas burner soots &ndash; along with surrogate black carbon, various multiwall carbon nanotube aggregate materials, TiO2 (anatase) and chrysotile asbestos as reference materials. All soots were observed utilizing TEM and FESEM to be composed of aggregated, primary spherules (20&ndash;80 nm diameter) forming complex, branched fractal structures. These spherules were composed of intercalated, turbostratic arrangements of curved graphene fragments with varying concentrations of polycyclic aromatic hydrocarbon (PAH) isomers. In vitro cultures with an immortalized human lung epithelial carcinoma cell line (A549) treated with these materials showed decreased cell viability and variations in ROS production, with no correlations to PAH content. The data demonstrate that soots are cytotoxic and that cytotoxicity is not related to PAH content but is related to ROS generation, suggesting that soot induces cellular oxidative stress and that cell viability assays can be indicators of ROS production.Keywords: cytotoxicity assessment, ROS assays, FESEM and TEM analysis, nanoparticulate aggregates
format	article
author	Kristine M Garza Karla F Soto Lawrence E Murr
author_facet	Kristine M Garza Karla F Soto Lawrence E Murr
author_sort	Kristine M Garza
title	Cytotoxicity and reactive oxygen species generation from aggregated carbon and carbonaceous nanoparticulate materials
title_short	Cytotoxicity and reactive oxygen species generation from aggregated carbon and carbonaceous nanoparticulate materials
title_full	Cytotoxicity and reactive oxygen species generation from aggregated carbon and carbonaceous nanoparticulate materials
title_fullStr	Cytotoxicity and reactive oxygen species generation from aggregated carbon and carbonaceous nanoparticulate materials
title_full_unstemmed	Cytotoxicity and reactive oxygen species generation from aggregated carbon and carbonaceous nanoparticulate materials
title_sort	cytotoxicity and reactive oxygen species generation from aggregated carbon and carbonaceous nanoparticulate materials
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/a7f270e01cd141959fbb20e21806ed90
work_keys_str_mv	AT kristinemgarza cytotoxicityandreactiveoxygenspeciesgenerationfromaggregatedcarbonandcarbonaceousnanoparticulatematerials AT karlafsoto cytotoxicityandreactiveoxygenspeciesgenerationfromaggregatedcarbonandcarbonaceousnanoparticulatematerials AT lawrenceemurr cytotoxicityandreactiveoxygenspeciesgenerationfromaggregatedcarbonandcarbonaceousnanoparticulatematerials
_version_	1718402667718377472

Cytotoxicity and reactive oxygen species generation from aggregated carbon and carbonaceous nanoparticulate materials

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