KM55 in the evaluation of IgA containing glomerular diseases

Introduction: Mucosal derived galactose deficient IgA is central to the pathogenesis of primary IgA nephropathy. Recent reports suggest similar pathogenesis in Henoch Schonlein purpura and secondary IgA nephropathy. Its role in other IgA containing glomerular diseases is still under investigation. I...

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Autores principales:	Rahul Raj, Alok Sharma, Adarsh Barwad, Soumita Bagchi, Sanjay Kumar Agarwal, Arvind Bagga, Amit Kumar Dinda, Geetika Singh
Formato:	article
Lenguaje:	EN
Publicado:	Karger Publishers 2021
Materias:	Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/a7f3a91d4aa0491486e02b9ac65e8088
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spelling	oai:doaj.org-article:a7f3a91d4aa0491486e02b9ac65e80882021-12-02T12:40:24ZKM55 in the evaluation of IgA containing glomerular diseases2673-363310.1159/000520640https://doaj.org/article/a7f3a91d4aa0491486e02b9ac65e80882021-11-01T00:00:00Zhttps://www.karger.com/Article/FullText/520640https://doaj.org/toc/2673-3633Introduction: Mucosal derived galactose deficient IgA is central to the pathogenesis of primary IgA nephropathy. Recent reports suggest similar pathogenesis in Henoch Schonlein purpura and secondary IgA nephropathy. Its role in other IgA containing glomerular diseases is still under investigation. It can be detected in glomeruli with the recently described antibody, KM55. We aimed to evaluate the role of KM55 by immunostaining a wide spectrum of IgA containing glomerular diseases. Methods: After standardization and co localization in a case of IgA nephropathy, a spectrum of 60 cases including IgA nephropathy, Henoch Schonlein purpura, chronic liver disease related IgA nephropathy, other secondary IgA Nephropathy, IgA dominant/co dominant membranoproliferative glomerulonephritis and lupus nephritis were subjected to immunofluorescence with KM55. KM55 was used to resolve diagnostic dilemma in cases of IgA deposition with confounding histology. Results: The group of primary IgA Nephropathy (17 cases), Henoch Schonlein purpura (4 cases) and secondary IgA nephropathy (19 cases) including chronic liver disease showed 2 -3+ granular staining with KM55 suggesting mucosal derived IgA. In contrast, cases of IgA dominant/co dominant membranoproliferative glomerulonephritis (8 cases) and lupus nephritis (12 cases) were negative for KM55, suggesting systemic derivation of IgA. In cases of IgA deposition with confounding histology such as membranoproliferative or diffuse endocapillary proliferative pattern, KM55 helped to resolve the diagnosis. Discussion/Conclusion: This cross-sectional study concludes that KM55 is useful in the evaluation of IgA containing glomerular diseases from a pathogenetic perspective, and is a practical tool in resolving differential diagnosis in cases with overlapping histopathologic features.Rahul RajAlok SharmaAdarsh BarwadSoumita BagchiSanjay Kumar AgarwalArvind BaggaAmit Kumar DindaGeetika SinghKarger PublishersarticleDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENGlomerular Diseases (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	Diseases of the endocrine glands. Clinical endocrinology RC648-665 Rahul Raj Alok Sharma Adarsh Barwad Soumita Bagchi Sanjay Kumar Agarwal Arvind Bagga Amit Kumar Dinda Geetika Singh KM55 in the evaluation of IgA containing glomerular diseases
description	Introduction: Mucosal derived galactose deficient IgA is central to the pathogenesis of primary IgA nephropathy. Recent reports suggest similar pathogenesis in Henoch Schonlein purpura and secondary IgA nephropathy. Its role in other IgA containing glomerular diseases is still under investigation. It can be detected in glomeruli with the recently described antibody, KM55. We aimed to evaluate the role of KM55 by immunostaining a wide spectrum of IgA containing glomerular diseases. Methods: After standardization and co localization in a case of IgA nephropathy, a spectrum of 60 cases including IgA nephropathy, Henoch Schonlein purpura, chronic liver disease related IgA nephropathy, other secondary IgA Nephropathy, IgA dominant/co dominant membranoproliferative glomerulonephritis and lupus nephritis were subjected to immunofluorescence with KM55. KM55 was used to resolve diagnostic dilemma in cases of IgA deposition with confounding histology. Results: The group of primary IgA Nephropathy (17 cases), Henoch Schonlein purpura (4 cases) and secondary IgA nephropathy (19 cases) including chronic liver disease showed 2 -3+ granular staining with KM55 suggesting mucosal derived IgA. In contrast, cases of IgA dominant/co dominant membranoproliferative glomerulonephritis (8 cases) and lupus nephritis (12 cases) were negative for KM55, suggesting systemic derivation of IgA. In cases of IgA deposition with confounding histology such as membranoproliferative or diffuse endocapillary proliferative pattern, KM55 helped to resolve the diagnosis. Discussion/Conclusion: This cross-sectional study concludes that KM55 is useful in the evaluation of IgA containing glomerular diseases from a pathogenetic perspective, and is a practical tool in resolving differential diagnosis in cases with overlapping histopathologic features.
format	article
author	Rahul Raj Alok Sharma Adarsh Barwad Soumita Bagchi Sanjay Kumar Agarwal Arvind Bagga Amit Kumar Dinda Geetika Singh
author_facet	Rahul Raj Alok Sharma Adarsh Barwad Soumita Bagchi Sanjay Kumar Agarwal Arvind Bagga Amit Kumar Dinda Geetika Singh
author_sort	Rahul Raj
title	KM55 in the evaluation of IgA containing glomerular diseases
title_short	KM55 in the evaluation of IgA containing glomerular diseases
title_full	KM55 in the evaluation of IgA containing glomerular diseases
title_fullStr	KM55 in the evaluation of IgA containing glomerular diseases
title_full_unstemmed	KM55 in the evaluation of IgA containing glomerular diseases
title_sort	km55 in the evaluation of iga containing glomerular diseases
publisher	Karger Publishers
publishDate	2021
url	https://doaj.org/article/a7f3a91d4aa0491486e02b9ac65e8088
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KM55 in the evaluation of IgA containing glomerular diseases

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