Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats

Ayman Atiba,1 Tamer Wasfy,2 Walied Abdo,3 Ahmed Ghoneim,2 Tarek Kamal,4 Mustafa Shukry5 1Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 2Department of Ophthalmology, Faculty of Medicine, Tanta University, 3Department of Pa...

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Autores principales:	Atiba A, Wasfy T, Abdo W, Ghoneim A, Kamal T, Shukry M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/a7f44fe952af4407b56f630257ed08e5
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spelling	oai:doaj.org-article:a7f44fe952af4407b56f630257ed08e52021-12-02T08:36:00ZAloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats1177-5483https://doaj.org/article/a7f44fe952af4407b56f630257ed08e52015-10-01T00:00:00Zhttps://www.dovepress.com/aloe-vera-gel-facilitates-re-epithelialization-of-corneal-alkali-burn--peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Ayman Atiba,1 Tamer Wasfy,2 Walied Abdo,3 Ahmed Ghoneim,2 Tarek Kamal,4 Mustafa Shukry5 1Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 2Department of Ophthalmology, Faculty of Medicine, Tanta University, 3Department of Pathology, 4Department of Biochemistry, 5Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt Purpose: To investigate the efficacy of topical applied aloe vera (AV) and to facilitate the repair of the standardized alkaline corneal ulcer in normal and diabetic rats.Materials and methods: The corneal alkali-burn injury model was established unilaterally in Wistar rats by filter paper saturated with 0.01 M NaOH contacting the eyes for 45 seconds. Rats were divided into four groups: normal control (NC), normal AV (NAV), diabetic control (DC), and diabetic AV (DAV). NAV and DAV groups were treated with AV gel eye drops four times daily, and NC and DC groups were treated with normal saline for 3 days. Corneal epithelial wound closure and degree of edema were recorded using slit lamp and optical coherence tomography at 0, 24, 48, and 72 hours postwounding. Histological examination was conducted to evaluate the degree of inflammation and the healing effect.Results: Corneal epithelial wound healing was better in the NAV group than in the NC group, and it was significantly higher in the DAV group than in the DC group (P<0.05). In comparison to the DC group, DAV treated with AV demonstrated a marked reduction in edema at 48 and 72 hours. Histologically, corneal re-epithelialization was complete and higher in DAV group than that in DC group; moreover, the inflammatory cells were increased in DC group than DAV group (P<0.05).Conclusion: This study demonstrated the efficacy of AV for enhanced corneal re-epithelialization, as well as reduced inflammatory response after alkali burn in rats; therefore, it could be useful as a therapy for diabetic keratopathy. Keywords: aloe vera, cornea, epithelium, diabetes, wound healingAtiba AWasfy TAbdo WGhoneim AKamal TShukry MDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2015, Iss default, Pp 2019-2026 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Atiba A Wasfy T Abdo W Ghoneim A Kamal T Shukry M Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats
description	Ayman Atiba,1 Tamer Wasfy,2 Walied Abdo,3 Ahmed Ghoneim,2 Tarek Kamal,4 Mustafa Shukry5 1Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 2Department of Ophthalmology, Faculty of Medicine, Tanta University, 3Department of Pathology, 4Department of Biochemistry, 5Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt Purpose: To investigate the efficacy of topical applied aloe vera (AV) and to facilitate the repair of the standardized alkaline corneal ulcer in normal and diabetic rats.Materials and methods: The corneal alkali-burn injury model was established unilaterally in Wistar rats by filter paper saturated with 0.01 M NaOH contacting the eyes for 45 seconds. Rats were divided into four groups: normal control (NC), normal AV (NAV), diabetic control (DC), and diabetic AV (DAV). NAV and DAV groups were treated with AV gel eye drops four times daily, and NC and DC groups were treated with normal saline for 3 days. Corneal epithelial wound closure and degree of edema were recorded using slit lamp and optical coherence tomography at 0, 24, 48, and 72 hours postwounding. Histological examination was conducted to evaluate the degree of inflammation and the healing effect.Results: Corneal epithelial wound healing was better in the NAV group than in the NC group, and it was significantly higher in the DAV group than in the DC group (P<0.05). In comparison to the DC group, DAV treated with AV demonstrated a marked reduction in edema at 48 and 72 hours. Histologically, corneal re-epithelialization was complete and higher in DAV group than that in DC group; moreover, the inflammatory cells were increased in DC group than DAV group (P<0.05).Conclusion: This study demonstrated the efficacy of AV for enhanced corneal re-epithelialization, as well as reduced inflammatory response after alkali burn in rats; therefore, it could be useful as a therapy for diabetic keratopathy. Keywords: aloe vera, cornea, epithelium, diabetes, wound healing
format	article
author	Atiba A Wasfy T Abdo W Ghoneim A Kamal T Shukry M
author_facet	Atiba A Wasfy T Abdo W Ghoneim A Kamal T Shukry M
author_sort	Atiba A
title	Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats
title_short	Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats
title_full	Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats
title_fullStr	Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats
title_full_unstemmed	Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats
title_sort	aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/a7f44fe952af4407b56f630257ed08e5
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Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats

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