MitoQ inhibits hepatic stellate cell activation and liver fibrosis by enhancing PINK1/parkin-mediated mitophagy

Mitophagy affects the activation of hepatic stellate cells (HSCs). Mitochondria-targeted ubiquinone (MitoQ) is a mitochondria-targeted antioxidant that reduces the production of intracellular reactive oxygen species (ROS). However, its relationship with mitophagy remains unclear. This study evaluate...

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Autores principales:	Dou Shi-Ying, Zhang Jiu-Na, Xie Xiao-Li, Liu Ting, Hu Jun-Li, Jiang Xiao-Yu, Wang Miao-Miao, Jiang Hui-Qing
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	liver fibrosis hepatic stellate cell ubiquinone pink1 mitophagy Medicine R
Acceso en línea:	https://doaj.org/article/a7f568f118f84e518080078bd6a978b4
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spelling	oai:doaj.org-article:a7f568f118f84e518080078bd6a978b42021-12-05T14:10:55ZMitoQ inhibits hepatic stellate cell activation and liver fibrosis by enhancing PINK1/parkin-mediated mitophagy2391-546310.1515/med-2021-0394https://doaj.org/article/a7f568f118f84e518080078bd6a978b42021-11-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0394https://doaj.org/toc/2391-5463Mitophagy affects the activation of hepatic stellate cells (HSCs). Mitochondria-targeted ubiquinone (MitoQ) is a mitochondria-targeted antioxidant that reduces the production of intracellular reactive oxygen species (ROS). However, its relationship with mitophagy remains unclear. This study evaluated mitophagy during HSC activation and the effects of MitoQ on mitophagy in cell culture and in an animal model of the activation of HSCs. We found that MitoQ reduced the activation of HSCs and alleviated hepatic fibrosis. PINK1 (PTEN-induced putative kinase 1) is a putative serine/threonine kinase located in the mitochondria’s outer membrane. While the activation of primary HSCs or LX-2 cells was associated with reduced PINK1/parkin-mediated mitophagy, MitoQ reduced intracellular ROS levels, enhanced PINK1/parkin-mediated mitophagy, and inhibited the activation of HSCs. After knocking down the key mitophagy-related protein, PINK1, in LX-2 cells to block mitophagy, MitoQ intervention failed to inhibit HSC activation. Our results showed that MitoQ inhibited the activation of HSCs and alleviated hepatic fibrosis by enhancing PINK1/parkin-mediated mitophagy.Dou Shi-YingZhang Jiu-NaXie Xiao-LiLiu TingHu Jun-LiJiang Xiao-YuWang Miao-MiaoJiang Hui-QingDe Gruyterarticleliver fibrosishepatic stellate cellubiquinonepink1 mitophagyMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 1718-1727 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	liver fibrosis hepatic stellate cell ubiquinone pink1 mitophagy Medicine R
spellingShingle	liver fibrosis hepatic stellate cell ubiquinone pink1 mitophagy Medicine R Dou Shi-Ying Zhang Jiu-Na Xie Xiao-Li Liu Ting Hu Jun-Li Jiang Xiao-Yu Wang Miao-Miao Jiang Hui-Qing MitoQ inhibits hepatic stellate cell activation and liver fibrosis by enhancing PINK1/parkin-mediated mitophagy
description	Mitophagy affects the activation of hepatic stellate cells (HSCs). Mitochondria-targeted ubiquinone (MitoQ) is a mitochondria-targeted antioxidant that reduces the production of intracellular reactive oxygen species (ROS). However, its relationship with mitophagy remains unclear. This study evaluated mitophagy during HSC activation and the effects of MitoQ on mitophagy in cell culture and in an animal model of the activation of HSCs. We found that MitoQ reduced the activation of HSCs and alleviated hepatic fibrosis. PINK1 (PTEN-induced putative kinase 1) is a putative serine/threonine kinase located in the mitochondria’s outer membrane. While the activation of primary HSCs or LX-2 cells was associated with reduced PINK1/parkin-mediated mitophagy, MitoQ reduced intracellular ROS levels, enhanced PINK1/parkin-mediated mitophagy, and inhibited the activation of HSCs. After knocking down the key mitophagy-related protein, PINK1, in LX-2 cells to block mitophagy, MitoQ intervention failed to inhibit HSC activation. Our results showed that MitoQ inhibited the activation of HSCs and alleviated hepatic fibrosis by enhancing PINK1/parkin-mediated mitophagy.
format	article
author	Dou Shi-Ying Zhang Jiu-Na Xie Xiao-Li Liu Ting Hu Jun-Li Jiang Xiao-Yu Wang Miao-Miao Jiang Hui-Qing
author_facet	Dou Shi-Ying Zhang Jiu-Na Xie Xiao-Li Liu Ting Hu Jun-Li Jiang Xiao-Yu Wang Miao-Miao Jiang Hui-Qing
author_sort	Dou Shi-Ying
title	MitoQ inhibits hepatic stellate cell activation and liver fibrosis by enhancing PINK1/parkin-mediated mitophagy
title_short	MitoQ inhibits hepatic stellate cell activation and liver fibrosis by enhancing PINK1/parkin-mediated mitophagy
title_full	MitoQ inhibits hepatic stellate cell activation and liver fibrosis by enhancing PINK1/parkin-mediated mitophagy
title_fullStr	MitoQ inhibits hepatic stellate cell activation and liver fibrosis by enhancing PINK1/parkin-mediated mitophagy
title_full_unstemmed	MitoQ inhibits hepatic stellate cell activation and liver fibrosis by enhancing PINK1/parkin-mediated mitophagy
title_sort	mitoq inhibits hepatic stellate cell activation and liver fibrosis by enhancing pink1/parkin-mediated mitophagy
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/a7f568f118f84e518080078bd6a978b4
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MitoQ inhibits hepatic stellate cell activation and liver fibrosis by enhancing PINK1/parkin-mediated mitophagy

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