Effect of the Oxidative Phosphorylation Uncoupler <i>Para</i>-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor

In this work, the metabolic uncoupler <i>para</i>-nitrophenol (pNP) was applied to suppress excess sludge production and to investigate its effects on the system’s performance and activated sludge community structure. The COD removal efficiency decreased from 99.0% to 89.5% prior to and...

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Autores principales:	Ioannis Stavrakakis, Nikolaos Remmas, Paraschos Melidis, Spyridon Ntougias
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	metabolic uncoupler nitrophenols excess sludge nitrification and denitrification inhibition <i>Tetrasphaera</i> Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
Acceso en línea:	https://doaj.org/article/a7ffeb90a7cc409694d13e78e4726483
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spelling	oai:doaj.org-article:a7ffeb90a7cc409694d13e78e47264832021-11-25T19:15:41ZEffect of the Oxidative Phosphorylation Uncoupler <i>Para</i>-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor10.3390/w132232222073-4441https://doaj.org/article/a7ffeb90a7cc409694d13e78e47264832021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4441/13/22/3222https://doaj.org/toc/2073-4441In this work, the metabolic uncoupler <i>para</i>-nitrophenol (pNP) was applied to suppress excess sludge production and to investigate its effects on the system’s performance and activated sludge community structure. The COD removal efficiency decreased from 99.0% to 89.5% prior to and after pNP addition, respectively. Application of pNP transiently reduced NH<sub>4</sub><sup>+</sup>-N, NO<sub>3</sub><sup>−</sup>-N and NO<sub>2</sub><sup>−</sup>-N removal efficiencies, suggesting partial inhibition of both nitrifying and denitrifying activity. However, no changes in the relative abundance of the nitrifying bacteria occurred. Phosphorus removal efficiency was sharply reduced after pNP addition, as the consequence of hydrolysis of stored cell reserves. <i>Tetrasphaera</i>, a key polyphosphate accumulating organism, was also affected by the addition of pNP, a fact that highly influenced system’s ability to remove phosphorus. A drastic drop in Soluble Microbial Products (SMP) and Extracellular Polymeric Substances (EPS) was also detected shortly after the introduction of the uncoupler. On the other hand, MBR’s physicochemical parameters were restored to initial values a week after the addition of pNP. Moreover, remarkable changes in beta-diversity were noted after pNP addition. An increase of <i>Bacteroidetes</i>, <i>Gammaproteobacteria</i> and <i>Firmicutes</i> over <i>Actinobacteria</i> and <i>Alphaproteobacteria</i> was also observed after pNP addition.Ioannis StavrakakisNikolaos RemmasParaschos MelidisSpyridon NtougiasMDPI AGarticlemetabolic uncouplernitrophenolsexcess sludgenitrification and denitrification inhibition<i>Tetrasphaera</i>Hydraulic engineeringTC1-978Water supply for domestic and industrial purposesTD201-500ENWater, Vol 13, Iss 3222, p 3222 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	metabolic uncoupler nitrophenols excess sludge nitrification and denitrification inhibition <i>Tetrasphaera</i> Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500
spellingShingle	metabolic uncoupler nitrophenols excess sludge nitrification and denitrification inhibition <i>Tetrasphaera</i> Hydraulic engineering TC1-978 Water supply for domestic and industrial purposes TD201-500 Ioannis Stavrakakis Nikolaos Remmas Paraschos Melidis Spyridon Ntougias Effect of the Oxidative Phosphorylation Uncoupler <i>Para</i>-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor
description	In this work, the metabolic uncoupler <i>para</i>-nitrophenol (pNP) was applied to suppress excess sludge production and to investigate its effects on the system’s performance and activated sludge community structure. The COD removal efficiency decreased from 99.0% to 89.5% prior to and after pNP addition, respectively. Application of pNP transiently reduced NH<sub>4</sub><sup>+</sup>-N, NO<sub>3</sub><sup>−</sup>-N and NO<sub>2</sub><sup>−</sup>-N removal efficiencies, suggesting partial inhibition of both nitrifying and denitrifying activity. However, no changes in the relative abundance of the nitrifying bacteria occurred. Phosphorus removal efficiency was sharply reduced after pNP addition, as the consequence of hydrolysis of stored cell reserves. <i>Tetrasphaera</i>, a key polyphosphate accumulating organism, was also affected by the addition of pNP, a fact that highly influenced system’s ability to remove phosphorus. A drastic drop in Soluble Microbial Products (SMP) and Extracellular Polymeric Substances (EPS) was also detected shortly after the introduction of the uncoupler. On the other hand, MBR’s physicochemical parameters were restored to initial values a week after the addition of pNP. Moreover, remarkable changes in beta-diversity were noted after pNP addition. An increase of <i>Bacteroidetes</i>, <i>Gammaproteobacteria</i> and <i>Firmicutes</i> over <i>Actinobacteria</i> and <i>Alphaproteobacteria</i> was also observed after pNP addition.
format	article
author	Ioannis Stavrakakis Nikolaos Remmas Paraschos Melidis Spyridon Ntougias
author_facet	Ioannis Stavrakakis Nikolaos Remmas Paraschos Melidis Spyridon Ntougias
author_sort	Ioannis Stavrakakis
title	Effect of the Oxidative Phosphorylation Uncoupler <i>Para</i>-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor
title_short	Effect of the Oxidative Phosphorylation Uncoupler <i>Para</i>-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor
title_full	Effect of the Oxidative Phosphorylation Uncoupler <i>Para</i>-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor
title_fullStr	Effect of the Oxidative Phosphorylation Uncoupler <i>Para</i>-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor
title_full_unstemmed	Effect of the Oxidative Phosphorylation Uncoupler <i>Para</i>-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor
title_sort	effect of the oxidative phosphorylation uncoupler <i>para</i>-nitrophenol on the activated sludge community structure and performance of a submerged membrane bioreactor
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a7ffeb90a7cc409694d13e78e4726483
work_keys_str_mv	AT ioannisstavrakakis effectoftheoxidativephosphorylationuncoupleriparainitrophenolontheactivatedsludgecommunitystructureandperformanceofasubmergedmembranebioreactor AT nikolaosremmas effectoftheoxidativephosphorylationuncoupleriparainitrophenolontheactivatedsludgecommunitystructureandperformanceofasubmergedmembranebioreactor AT paraschosmelidis effectoftheoxidativephosphorylationuncoupleriparainitrophenolontheactivatedsludgecommunitystructureandperformanceofasubmergedmembranebioreactor AT spyridonntougias effectoftheoxidativephosphorylationuncoupleriparainitrophenolontheactivatedsludgecommunitystructureandperformanceofasubmergedmembranebioreactor
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Effect of the Oxidative Phosphorylation Uncoupler <i>Para</i>-Nitrophenol on the Activated Sludge Community Structure and Performance of a Submerged Membrane Bioreactor

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