The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation

Extracellular vesicles (EVs) are cell-derived membranous particles secreted by all cell types (including virus infected and uninfected cells) into the extracellular milieu. EVs carry, protect, and transport a wide array of bioactive cargoes to recipient/target cells. EVs regulate physiological and p...

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Autores principales:	Hussein Kaddour, Malik Tranquille, Chioma M. Okeoma
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	extracellular vesicles (EVs) exosomes size exclusion chromatography (SEC) gradient size exclusion chromatography (gSEC) ion exchange chromatography (IEX) hybrid chromatography Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a80830cdff024d958fb9645a9f37e070
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spelling	oai:doaj.org-article:a80830cdff024d958fb9645a9f37e0702021-11-25T19:13:59ZThe Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation10.3390/v131122721999-4915https://doaj.org/article/a80830cdff024d958fb9645a9f37e0702021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2272https://doaj.org/toc/1999-4915Extracellular vesicles (EVs) are cell-derived membranous particles secreted by all cell types (including virus infected and uninfected cells) into the extracellular milieu. EVs carry, protect, and transport a wide array of bioactive cargoes to recipient/target cells. EVs regulate physiological and pathophysiological processes in recipient cells and are important in therapeutics/drug delivery. Despite these great attributes of EVs, an efficient protocol for EV separation from biofluids is lacking. Numerous techniques have been adapted for the separation of EVs with size exclusion chromatography (SEC)-based methods being the most promising. Here, we review the SEC protocols used for EV separation, and discuss opportunities for significant improvements, such as the development of novel particle purification liquid chromatography (PPLC) system capable of tandem purification and characterization of biological and synthetic particles with near-single vesicle resolution. Finally, we identify future perspectives and current issues to make PPLC a tool capable of providing a unified, automated, adaptable, yet simple and affordable particle separation resource.Hussein KaddourMalik TranquilleChioma M. OkeomaMDPI AGarticleextracellular vesicles (EVs)exosomessize exclusion chromatography (SEC)gradient size exclusion chromatography (gSEC)ion exchange chromatography (IEX)hybrid chromatographyMicrobiologyQR1-502ENViruses, Vol 13, Iss 2272, p 2272 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular vesicles (EVs) exosomes size exclusion chromatography (SEC) gradient size exclusion chromatography (gSEC) ion exchange chromatography (IEX) hybrid chromatography Microbiology QR1-502
spellingShingle	extracellular vesicles (EVs) exosomes size exclusion chromatography (SEC) gradient size exclusion chromatography (gSEC) ion exchange chromatography (IEX) hybrid chromatography Microbiology QR1-502 Hussein Kaddour Malik Tranquille Chioma M. Okeoma The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation
description	Extracellular vesicles (EVs) are cell-derived membranous particles secreted by all cell types (including virus infected and uninfected cells) into the extracellular milieu. EVs carry, protect, and transport a wide array of bioactive cargoes to recipient/target cells. EVs regulate physiological and pathophysiological processes in recipient cells and are important in therapeutics/drug delivery. Despite these great attributes of EVs, an efficient protocol for EV separation from biofluids is lacking. Numerous techniques have been adapted for the separation of EVs with size exclusion chromatography (SEC)-based methods being the most promising. Here, we review the SEC protocols used for EV separation, and discuss opportunities for significant improvements, such as the development of novel particle purification liquid chromatography (PPLC) system capable of tandem purification and characterization of biological and synthetic particles with near-single vesicle resolution. Finally, we identify future perspectives and current issues to make PPLC a tool capable of providing a unified, automated, adaptable, yet simple and affordable particle separation resource.
format	article
author	Hussein Kaddour Malik Tranquille Chioma M. Okeoma
author_facet	Hussein Kaddour Malik Tranquille Chioma M. Okeoma
author_sort	Hussein Kaddour
title	The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation
title_short	The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation
title_full	The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation
title_fullStr	The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation
title_full_unstemmed	The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation
title_sort	past, the present, and the future of the size exclusion chromatography in extracellular vesicles separation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a80830cdff024d958fb9645a9f37e070
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The Past, the Present, and the Future of the Size Exclusion Chromatography in Extracellular Vesicles Separation

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