Green Curmin Reduces Pro-inflammatory Cytokines and Fibroblast-Associated Colon Cancer Migration

Background: Green Curmin is a soluble curcumin extract product made in Thailand that has been reported to reduce gastric inflammation. Inflammatory cytokines including IL-1α, IL-6,and TNF-β play a crucial role in cancer cell migration, which is a major development towards poor prognosis. The aim of...

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Autores principales:	Nasapon Povichit, Tharathip Muangthong, Parichaya Aimvijarn, Prasit Suwannalert
Formato:	article
Lenguaje:	EN
Publicado:	Tabriz University of Medical Sciences 2021
Materias:	green curmin inflammation colon cancer fibroblast migration Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/a809132bc1ab4e8f82cd31a88bbd710c
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spelling	oai:doaj.org-article:a809132bc1ab4e8f82cd31a88bbd710c2021-11-29T05:52:56ZGreen Curmin Reduces Pro-inflammatory Cytokines and Fibroblast-Associated Colon Cancer Migration2383-288610.34172/PS.2021.9https://doaj.org/article/a809132bc1ab4e8f82cd31a88bbd710c2021-12-01T00:00:00Zhttps://ps.tbzmed.ac.ir/PDF/ps-27-528.pdfhttps://doaj.org/toc/2383-2886Background: Green Curmin is a soluble curcumin extract product made in Thailand that has been reported to reduce gastric inflammation. Inflammatory cytokines including IL-1α, IL-6,and TNF-β play a crucial role in cancer cell migration, which is a major development towards poor prognosis. The aim of this study is to investigate the effects of Green Curmin on colon cancer cell growth and migration, which involve pro-inflammatory cytokines. Methods: Curcuma longa L. were extracted and named Green Curmin. The cytotoxicity of theGreen Curmin-treated HCT116-colon cancer cells was obtained by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. The effect of the extract on cancer migration was investigated with a wound-healing assay. In addition, fibroblast-associated cancer migration was confirmed by using the Transwell migration assay. Proinflammatory cytokines were also determined. Results: Green Curmin had cytotoxicity in the induced-cancer cell apoptosis at concentrationsof 1 and 5 mg/ml. However, reduced colon cancer migration and fibroblast associated-cancermigration was found at concentrations of 0.05 and 0.1 mg/ml (non-toxicity dose), respectively. They also suppressed pro-inflammatory cytokines expression including IL-1α, IL-6, and TNF-βin colon cancer cells and IL-6 in fibroblast cells. Conclusion: Green Curmin has the potential to suppress pro-inflammatory cytokines and reduce fibroblast associated-cancer migration.Nasapon PovichitTharathip MuangthongParichaya AimvijarnPrasit SuwannalertTabriz University of Medical Sciencesarticlegreen curmininflammationcolon cancerfibroblastmigrationPharmacy and materia medicaRS1-441ENPharmaceutical Sciences, Vol 27, Iss 4, Pp 528-535 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	green curmin inflammation colon cancer fibroblast migration Pharmacy and materia medica RS1-441
spellingShingle	green curmin inflammation colon cancer fibroblast migration Pharmacy and materia medica RS1-441 Nasapon Povichit Tharathip Muangthong Parichaya Aimvijarn Prasit Suwannalert Green Curmin Reduces Pro-inflammatory Cytokines and Fibroblast-Associated Colon Cancer Migration
description	Background: Green Curmin is a soluble curcumin extract product made in Thailand that has been reported to reduce gastric inflammation. Inflammatory cytokines including IL-1α, IL-6,and TNF-β play a crucial role in cancer cell migration, which is a major development towards poor prognosis. The aim of this study is to investigate the effects of Green Curmin on colon cancer cell growth and migration, which involve pro-inflammatory cytokines. Methods: Curcuma longa L. were extracted and named Green Curmin. The cytotoxicity of theGreen Curmin-treated HCT116-colon cancer cells was obtained by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. The effect of the extract on cancer migration was investigated with a wound-healing assay. In addition, fibroblast-associated cancer migration was confirmed by using the Transwell migration assay. Proinflammatory cytokines were also determined. Results: Green Curmin had cytotoxicity in the induced-cancer cell apoptosis at concentrationsof 1 and 5 mg/ml. However, reduced colon cancer migration and fibroblast associated-cancermigration was found at concentrations of 0.05 and 0.1 mg/ml (non-toxicity dose), respectively. They also suppressed pro-inflammatory cytokines expression including IL-1α, IL-6, and TNF-βin colon cancer cells and IL-6 in fibroblast cells. Conclusion: Green Curmin has the potential to suppress pro-inflammatory cytokines and reduce fibroblast associated-cancer migration.
format	article
author	Nasapon Povichit Tharathip Muangthong Parichaya Aimvijarn Prasit Suwannalert
author_facet	Nasapon Povichit Tharathip Muangthong Parichaya Aimvijarn Prasit Suwannalert
author_sort	Nasapon Povichit
title	Green Curmin Reduces Pro-inflammatory Cytokines and Fibroblast-Associated Colon Cancer Migration
title_short	Green Curmin Reduces Pro-inflammatory Cytokines and Fibroblast-Associated Colon Cancer Migration
title_full	Green Curmin Reduces Pro-inflammatory Cytokines and Fibroblast-Associated Colon Cancer Migration
title_fullStr	Green Curmin Reduces Pro-inflammatory Cytokines and Fibroblast-Associated Colon Cancer Migration
title_full_unstemmed	Green Curmin Reduces Pro-inflammatory Cytokines and Fibroblast-Associated Colon Cancer Migration
title_sort	green curmin reduces pro-inflammatory cytokines and fibroblast-associated colon cancer migration
publisher	Tabriz University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/a809132bc1ab4e8f82cd31a88bbd710c
work_keys_str_mv	AT nasaponpovichit greencurminreducesproinflammatorycytokinesandfibroblastassociatedcoloncancermigration AT tharathipmuangthong greencurminreducesproinflammatorycytokinesandfibroblastassociatedcoloncancermigration AT parichayaaimvijarn greencurminreducesproinflammatorycytokinesandfibroblastassociatedcoloncancermigration AT prasitsuwannalert greencurminreducesproinflammatorycytokinesandfibroblastassociatedcoloncancermigration
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Green Curmin Reduces Pro-inflammatory Cytokines and Fibroblast-Associated Colon Cancer Migration

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