Knockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway

Sepsis is a common and lethal syndrome. Long non-coding RNA (lncRNA) transcript predicting survival in AKI (TapSAKI) has recently been found to serve as an important regulator in sepsis. However, the underlying mechanism of TapSAKI in sepsis pathogenesis remains largely unknown. Our data demonstrate...

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Autores principales:	Han Xiaoning, Yuan Zhiyong, Jing Yajun, Zhou Weigui, Sun Yunbo, Xing Jinyan
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	sepsis tapsaki mir-205 lps cell injury Medicine R
Acceso en línea:	https://doaj.org/article/a80d9cb1a5044e299f6401115dc30a30
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spelling	oai:doaj.org-article:a80d9cb1a5044e299f6401115dc30a302021-12-05T14:10:53ZKnockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway2391-546310.1515/med-2021-0204https://doaj.org/article/a80d9cb1a5044e299f6401115dc30a302021-04-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0204https://doaj.org/toc/2391-5463Sepsis is a common and lethal syndrome. Long non-coding RNA (lncRNA) transcript predicting survival in AKI (TapSAKI) has recently been found to serve as an important regulator in sepsis. However, the underlying mechanism of TapSAKI in sepsis pathogenesis remains largely unknown. Our data demonstrated that lipopolysaccharide (LPS)-induced HK-2 cell injury by weakening cell viability and enhancing cell apoptosis and inflammation. TapSAKI was upregulated and miR-205 was downregulated in LPS-induced HK-2 cells. TapSAKI knockdown or miR-205 overexpression alleviated LPS-induced cytotoxicity in HK-2 cells. TapSAKI sequestered miR-205 via acting as a miR-205 sponge. Moreover, the mitigating effect of TapSAKI silencing on LPS-induced HK-2 cell injury was mediated by miR-205. Additionally, the interferon regulatory factor 3 (IRF3) signaling was involved in the regulation of the TapSAKI/miR-205 axis on LPS-induced HK-2 cell damage. Our current study suggested that TapSAKI silencing relieved LPS-induced injury in HK-2 cells at least in part by sponging miR-205 and regulating the IRF3 signaling pathway, highlighting a novel understanding for sepsis pathogenesis and a promising target for this disease treatment.Han XiaoningYuan ZhiyongJing YajunZhou WeiguiSun YunboXing JinyanDe Gruyterarticlesepsistapsakimir-205lpscell injuryMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 581-590 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sepsis tapsaki mir-205 lps cell injury Medicine R
spellingShingle	sepsis tapsaki mir-205 lps cell injury Medicine R Han Xiaoning Yuan Zhiyong Jing Yajun Zhou Weigui Sun Yunbo Xing Jinyan Knockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway
description	Sepsis is a common and lethal syndrome. Long non-coding RNA (lncRNA) transcript predicting survival in AKI (TapSAKI) has recently been found to serve as an important regulator in sepsis. However, the underlying mechanism of TapSAKI in sepsis pathogenesis remains largely unknown. Our data demonstrated that lipopolysaccharide (LPS)-induced HK-2 cell injury by weakening cell viability and enhancing cell apoptosis and inflammation. TapSAKI was upregulated and miR-205 was downregulated in LPS-induced HK-2 cells. TapSAKI knockdown or miR-205 overexpression alleviated LPS-induced cytotoxicity in HK-2 cells. TapSAKI sequestered miR-205 via acting as a miR-205 sponge. Moreover, the mitigating effect of TapSAKI silencing on LPS-induced HK-2 cell injury was mediated by miR-205. Additionally, the interferon regulatory factor 3 (IRF3) signaling was involved in the regulation of the TapSAKI/miR-205 axis on LPS-induced HK-2 cell damage. Our current study suggested that TapSAKI silencing relieved LPS-induced injury in HK-2 cells at least in part by sponging miR-205 and regulating the IRF3 signaling pathway, highlighting a novel understanding for sepsis pathogenesis and a promising target for this disease treatment.
format	article
author	Han Xiaoning Yuan Zhiyong Jing Yajun Zhou Weigui Sun Yunbo Xing Jinyan
author_facet	Han Xiaoning Yuan Zhiyong Jing Yajun Zhou Weigui Sun Yunbo Xing Jinyan
author_sort	Han Xiaoning
title	Knockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway
title_short	Knockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway
title_full	Knockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway
title_fullStr	Knockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway
title_full_unstemmed	Knockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway
title_sort	knockdown of lncrna tapsaki alleviates lps-induced injury in hk-2 cells through the mir-205/irf3 pathway
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/a80d9cb1a5044e299f6401115dc30a30
work_keys_str_mv	AT hanxiaoning knockdownoflncrnatapsakialleviateslpsinducedinjuryinhk2cellsthroughthemir205irf3pathway AT yuanzhiyong knockdownoflncrnatapsakialleviateslpsinducedinjuryinhk2cellsthroughthemir205irf3pathway AT jingyajun knockdownoflncrnatapsakialleviateslpsinducedinjuryinhk2cellsthroughthemir205irf3pathway AT zhouweigui knockdownoflncrnatapsakialleviateslpsinducedinjuryinhk2cellsthroughthemir205irf3pathway AT sunyunbo knockdownoflncrnatapsakialleviateslpsinducedinjuryinhk2cellsthroughthemir205irf3pathway AT xingjinyan knockdownoflncrnatapsakialleviateslpsinducedinjuryinhk2cellsthroughthemir205irf3pathway
_version_	1718371621366923264

Knockdown of lncRNA TapSAKI alleviates LPS-induced injury in HK-2 cells through the miR-205/IRF3 pathway

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