In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa

Abstract Freeze-drying (FD) is a new and alternative method to preserve spermatozoa in refrigeration or at room temperature. Suitable protection is required to maintain the sperm DNA integrity during the whole process and storage. The aim of this study was to examine the effect of rosmarinic acid an...

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Autores principales:	Maite Olaciregui, Victoria Luño, Paula Domingo, Noelia González, Lydia Gil
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a816505605cf49fdb70cc4b5e6b6c209
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spelling	oai:doaj.org-article:a816505605cf49fdb70cc4b5e6b6c2092021-12-02T16:06:30ZIn vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa10.1038/s41598-017-00583-02045-2322https://doaj.org/article/a816505605cf49fdb70cc4b5e6b6c2092017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00583-0https://doaj.org/toc/2045-2322Abstract Freeze-drying (FD) is a new and alternative method to preserve spermatozoa in refrigeration or at room temperature. Suitable protection is required to maintain the sperm DNA integrity during the whole process and storage. The aim of this study was to examine the effect of rosmarinic acid and storage temperature on the DNA integrity of freeze-dried ram sperm. In addition, we evaluated the in vitro developmental ability to the blastocyst stage of oocytes injected with freeze-dried sperm. Ram sperm was freeze-dried in basic medium and in this medium supplemented with 105 µM rosmarinic acid. The vials were stored for 1 year at 4 °C and at room temperature. Frozen sperm was used as control. After rehydration, sperm DNA damage was evaluated, observing that the percentage of spermatozoa with DNA damage decreased significantly in the presence of rosmarinic acid, without differences between the two storage temperatures. Moreover, no differences were observed between the freeze-dried group and the frozen-thawed group in terms of blastocyst formation rate. We proved for the first time that ovine spermatozoa can be lyophilized effectively, stored at room temperature for long term, reconstituted and further injected into oocytes with initial embryo development.Maite OlacireguiVictoria LuñoPaula DomingoNoelia GonzálezLydia GilNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maite Olaciregui Victoria Luño Paula Domingo Noelia González Lydia Gil In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa
description	Abstract Freeze-drying (FD) is a new and alternative method to preserve spermatozoa in refrigeration or at room temperature. Suitable protection is required to maintain the sperm DNA integrity during the whole process and storage. The aim of this study was to examine the effect of rosmarinic acid and storage temperature on the DNA integrity of freeze-dried ram sperm. In addition, we evaluated the in vitro developmental ability to the blastocyst stage of oocytes injected with freeze-dried sperm. Ram sperm was freeze-dried in basic medium and in this medium supplemented with 105 µM rosmarinic acid. The vials were stored for 1 year at 4 °C and at room temperature. Frozen sperm was used as control. After rehydration, sperm DNA damage was evaluated, observing that the percentage of spermatozoa with DNA damage decreased significantly in the presence of rosmarinic acid, without differences between the two storage temperatures. Moreover, no differences were observed between the freeze-dried group and the frozen-thawed group in terms of blastocyst formation rate. We proved for the first time that ovine spermatozoa can be lyophilized effectively, stored at room temperature for long term, reconstituted and further injected into oocytes with initial embryo development.
format	article
author	Maite Olaciregui Victoria Luño Paula Domingo Noelia González Lydia Gil
author_facet	Maite Olaciregui Victoria Luño Paula Domingo Noelia González Lydia Gil
author_sort	Maite Olaciregui
title	In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa
title_short	In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa
title_full	In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa
title_fullStr	In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa
title_full_unstemmed	In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa
title_sort	in vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a816505605cf49fdb70cc4b5e6b6c209
work_keys_str_mv	AT maiteolaciregui invitrodevelopmentalabilityofovineoocytesfollowingintracytoplasmicinjectionwithfreezedriedspermatozoa AT victorialuno invitrodevelopmentalabilityofovineoocytesfollowingintracytoplasmicinjectionwithfreezedriedspermatozoa AT pauladomingo invitrodevelopmentalabilityofovineoocytesfollowingintracytoplasmicinjectionwithfreezedriedspermatozoa AT noeliagonzalez invitrodevelopmentalabilityofovineoocytesfollowingintracytoplasmicinjectionwithfreezedriedspermatozoa AT lydiagil invitrodevelopmentalabilityofovineoocytesfollowingintracytoplasmicinjectionwithfreezedriedspermatozoa
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In vitro developmental ability of ovine oocytes following intracytoplasmic injection with freeze-dried spermatozoa

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