Construction and application of GO<i>x</i>-based nanoreactor

Proteases play an important role in regulating and maintaining the normal function of the living organisms, and are widely used in the diagnosis and treatment of diseases. However, the natural protease is easily deactivated, denatured, and unable to enter cells directly, which greatly limit its appl...

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Autores principales:	HUANG Yan, LIU Jiahui, HOU Jiaxin, LYU Min, CHEN Nan
Formato:	article
Lenguaje:	EN ZH
Publicado:	Academic Journals Center of Shanghai Normal University 2021
Materias:	nanoreactor metal-organic zeolite-like material glucose oxidase(go<i>x</i>) tumor therapy Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/a816600cb6384740a8fac060a742a3f9
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spelling	oai:doaj.org-article:a816600cb6384740a8fac060a742a3f92021-11-12T03:34:00ZConstruction and application of GO<i>x</i>-based nanoreactor1000-513710.3969/J.ISSN.1000-5137.2021.05.007https://doaj.org/article/a816600cb6384740a8fac060a742a3f92021-10-01T00:00:00Zhttp://qktg.shnu.edu.cn/zrb/shsfqkszrb/ch/reader/view_abstract.aspx?file_no=20210507https://doaj.org/toc/1000-5137Proteases play an important role in regulating and maintaining the normal function of the living organisms, and are widely used in the diagnosis and treatment of diseases. However, the natural protease is easily deactivated, denatured, and unable to enter cells directly, which greatly limit its application and development. Therefore, it is urgent to construct efficient, safe nanocarriers that can deliver protease into cells. In the present work, a glucose oxidase (GO<i>x</i>)-based nanoreactor was constructed by synthesizing metal-organic zeolite-like nanoparticles with GO<i>x</i> as structural template. The nanoreactor has a high protein loading capacity, and can preserve the catalytic activity of GO<i>x</i>. Cytological experiments indicated that the nanoreactor can effectively enter tumor cells and locate in the lysosomes, consume intracellular glucose, and produce hydrogen peroxide, thus leading to the death of cancer cells. The nanoreactor constructed in this work represents a new safe and efficient platform for intracellular protein delivery.HUANG YanLIU JiahuiHOU JiaxinLYU MinCHEN NanAcademic Journals Center of Shanghai Normal Universityarticlenanoreactormetal-organic zeolite-like materialglucose oxidase(go<i>x</i>)tumor therapyScience (General)Q1-390ENZHJournal of Shanghai Normal University (Natural Sciences), Vol 50, Iss 5, Pp 567-574 (2021)
institution	DOAJ
collection	DOAJ
language	EN ZH
topic	nanoreactor metal-organic zeolite-like material glucose oxidase(go<i>x</i>) tumor therapy Science (General) Q1-390
spellingShingle	nanoreactor metal-organic zeolite-like material glucose oxidase(go<i>x</i>) tumor therapy Science (General) Q1-390 HUANG Yan LIU Jiahui HOU Jiaxin LYU Min CHEN Nan Construction and application of GO<i>x</i>-based nanoreactor
description	Proteases play an important role in regulating and maintaining the normal function of the living organisms, and are widely used in the diagnosis and treatment of diseases. However, the natural protease is easily deactivated, denatured, and unable to enter cells directly, which greatly limit its application and development. Therefore, it is urgent to construct efficient, safe nanocarriers that can deliver protease into cells. In the present work, a glucose oxidase (GO<i>x</i>)-based nanoreactor was constructed by synthesizing metal-organic zeolite-like nanoparticles with GO<i>x</i> as structural template. The nanoreactor has a high protein loading capacity, and can preserve the catalytic activity of GO<i>x</i>. Cytological experiments indicated that the nanoreactor can effectively enter tumor cells and locate in the lysosomes, consume intracellular glucose, and produce hydrogen peroxide, thus leading to the death of cancer cells. The nanoreactor constructed in this work represents a new safe and efficient platform for intracellular protein delivery.
format	article
author	HUANG Yan LIU Jiahui HOU Jiaxin LYU Min CHEN Nan
author_facet	HUANG Yan LIU Jiahui HOU Jiaxin LYU Min CHEN Nan
author_sort	HUANG Yan
title	Construction and application of GO<i>x</i>-based nanoreactor
title_short	Construction and application of GO<i>x</i>-based nanoreactor
title_full	Construction and application of GO<i>x</i>-based nanoreactor
title_fullStr	Construction and application of GO<i>x</i>-based nanoreactor
title_full_unstemmed	Construction and application of GO<i>x</i>-based nanoreactor
title_sort	construction and application of go<i>x</i>-based nanoreactor
publisher	Academic Journals Center of Shanghai Normal University
publishDate	2021
url	https://doaj.org/article/a816600cb6384740a8fac060a742a3f9
work_keys_str_mv	AT huangyan constructionandapplicationofgoixibasednanoreactor AT liujiahui constructionandapplicationofgoixibasednanoreactor AT houjiaxin constructionandapplicationofgoixibasednanoreactor AT lyumin constructionandapplicationofgoixibasednanoreactor AT chennan constructionandapplicationofgoixibasednanoreactor
_version_	1718431308429918208

Construction and application of GO<i>x</i>-based nanoreactor

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