The developmental transcriptome of the human heart

Abstract The human heart develops through complex mechanisms producing morphological and functional changes during gestation. We have recently demonstrated using diffusion tensor MRI that over the relatively short space of 40 days, between 100–140 days gestational age, the ventricular myocardium tra...

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Autores principales:	Eleftheria Pervolaraki, James Dachtler, Richard A. Anderson, Arun V. Holden
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Differentially Expressed Genes (DEGs) Human Heart Development Early Second Trimester Ordered Structure Characteristic Entrez Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a816a45747414ef29aae48ac371a0823
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id	oai:doaj.org-article:a816a45747414ef29aae48ac371a0823
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spelling	oai:doaj.org-article:a816a45747414ef29aae48ac371a08232021-12-02T15:07:51ZThe developmental transcriptome of the human heart10.1038/s41598-018-33837-62045-2322https://doaj.org/article/a816a45747414ef29aae48ac371a08232018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33837-6https://doaj.org/toc/2045-2322Abstract The human heart develops through complex mechanisms producing morphological and functional changes during gestation. We have recently demonstrated using diffusion tensor MRI that over the relatively short space of 40 days, between 100–140 days gestational age, the ventricular myocardium transforms from a disorganised tissue to the ordered structure characteristic of mature cardiac tissue. However, the genetic basis underpinning this maturation is unclear. Herein, we have used RNA-Seq to establish the developmentally-regulated transcriptome of gene expression in the developing human heart across three gestational ages in the first and second trimester. By comparing 9 weeks gestational age (WGA) with 12 WGA, we find 288 genes show significant differential expression. 305 genes were significantly altered comparing 12 and 16 WGA, and 806 genes differentially expressed between 9 and 16 WGA. Network analysis was used to identify genetic interactions, node properties and gene ontology categories. In summary, we present a comprehensive transcriptomic analysis of human heart development during early gestation, and identify differentially expressed genes during heart development between 9 and 16 weeks, overlapping the first and early second trimester.Eleftheria PervolarakiJames DachtlerRichard A. AndersonArun V. HoldenNature PortfolioarticleDifferentially Expressed Genes (DEGs)Human Heart DevelopmentEarly Second TrimesterOrdered Structure CharacteristicEntrezMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Differentially Expressed Genes (DEGs) Human Heart Development Early Second Trimester Ordered Structure Characteristic Entrez Medicine R Science Q
spellingShingle	Differentially Expressed Genes (DEGs) Human Heart Development Early Second Trimester Ordered Structure Characteristic Entrez Medicine R Science Q Eleftheria Pervolaraki James Dachtler Richard A. Anderson Arun V. Holden The developmental transcriptome of the human heart
description	Abstract The human heart develops through complex mechanisms producing morphological and functional changes during gestation. We have recently demonstrated using diffusion tensor MRI that over the relatively short space of 40 days, between 100–140 days gestational age, the ventricular myocardium transforms from a disorganised tissue to the ordered structure characteristic of mature cardiac tissue. However, the genetic basis underpinning this maturation is unclear. Herein, we have used RNA-Seq to establish the developmentally-regulated transcriptome of gene expression in the developing human heart across three gestational ages in the first and second trimester. By comparing 9 weeks gestational age (WGA) with 12 WGA, we find 288 genes show significant differential expression. 305 genes were significantly altered comparing 12 and 16 WGA, and 806 genes differentially expressed between 9 and 16 WGA. Network analysis was used to identify genetic interactions, node properties and gene ontology categories. In summary, we present a comprehensive transcriptomic analysis of human heart development during early gestation, and identify differentially expressed genes during heart development between 9 and 16 weeks, overlapping the first and early second trimester.
format	article
author	Eleftheria Pervolaraki James Dachtler Richard A. Anderson Arun V. Holden
author_facet	Eleftheria Pervolaraki James Dachtler Richard A. Anderson Arun V. Holden
author_sort	Eleftheria Pervolaraki
title	The developmental transcriptome of the human heart
title_short	The developmental transcriptome of the human heart
title_full	The developmental transcriptome of the human heart
title_fullStr	The developmental transcriptome of the human heart
title_full_unstemmed	The developmental transcriptome of the human heart
title_sort	developmental transcriptome of the human heart
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a816a45747414ef29aae48ac371a0823
work_keys_str_mv	AT eleftheriapervolaraki thedevelopmentaltranscriptomeofthehumanheart AT jamesdachtler thedevelopmentaltranscriptomeofthehumanheart AT richardaanderson thedevelopmentaltranscriptomeofthehumanheart AT arunvholden thedevelopmentaltranscriptomeofthehumanheart AT eleftheriapervolaraki developmentaltranscriptomeofthehumanheart AT jamesdachtler developmentaltranscriptomeofthehumanheart AT richardaanderson developmentaltranscriptomeofthehumanheart AT arunvholden developmentaltranscriptomeofthehumanheart
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The developmental transcriptome of the human heart

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