Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application.

RB1-inducible coiled-coil 1 (RB1CC1; also known as FIP200) plays important roles in several biological pathways such as cell proliferation and autophagy. Evaluation of RB1CC1 expression can provide useful clinical information on various cancers and neurodegenerative diseases. In order to realize the...

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Autores principales:	Yusuke Hama, Tokuhiro Chano, Takuma Inui, Kyoichi Matsumoto, Hidetoshi Okabe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a8176850734d4c13942308043e039a51
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spelling	oai:doaj.org-article:a8176850734d4c13942308043e039a512021-11-18T07:26:18ZPreparation of mouse monoclonal antibody for RB1CC1 and its clinical application.1932-620310.1371/journal.pone.0032052https://doaj.org/article/a8176850734d4c13942308043e039a512012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22396748/?tool=EBIhttps://doaj.org/toc/1932-6203RB1-inducible coiled-coil 1 (RB1CC1; also known as FIP200) plays important roles in several biological pathways such as cell proliferation and autophagy. Evaluation of RB1CC1 expression can provide useful clinical information on various cancers and neurodegenerative diseases. In order to realize the clinical applications, it is necessary to establish a stable supply of antibody and reproducible procedures for the laboratory examinations. In the present study, we have generated mouse monoclonal antibodies for RB1CC1, and four kinds of antibodies (N1-8, N1-216, N3-2, and N3-42) were found to be optimal for clinical applications such as ELISA and immunoblots and work as well as the pre-existing polyclonal antibodies. N1-8 monoclonal antibody provided the best recognition of RB1CC1 in the clinico-pathological examination of formalin-fixed paraffin-embedded tissues. These monoclonal antibodies will help to generate new opportunities in scientific examinations in biology and clinical medicine.Yusuke HamaTokuhiro ChanoTakuma InuiKyoichi MatsumotoHidetoshi OkabePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e32052 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yusuke Hama Tokuhiro Chano Takuma Inui Kyoichi Matsumoto Hidetoshi Okabe Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application.
description	RB1-inducible coiled-coil 1 (RB1CC1; also known as FIP200) plays important roles in several biological pathways such as cell proliferation and autophagy. Evaluation of RB1CC1 expression can provide useful clinical information on various cancers and neurodegenerative diseases. In order to realize the clinical applications, it is necessary to establish a stable supply of antibody and reproducible procedures for the laboratory examinations. In the present study, we have generated mouse monoclonal antibodies for RB1CC1, and four kinds of antibodies (N1-8, N1-216, N3-2, and N3-42) were found to be optimal for clinical applications such as ELISA and immunoblots and work as well as the pre-existing polyclonal antibodies. N1-8 monoclonal antibody provided the best recognition of RB1CC1 in the clinico-pathological examination of formalin-fixed paraffin-embedded tissues. These monoclonal antibodies will help to generate new opportunities in scientific examinations in biology and clinical medicine.
format	article
author	Yusuke Hama Tokuhiro Chano Takuma Inui Kyoichi Matsumoto Hidetoshi Okabe
author_facet	Yusuke Hama Tokuhiro Chano Takuma Inui Kyoichi Matsumoto Hidetoshi Okabe
author_sort	Yusuke Hama
title	Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application.
title_short	Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application.
title_full	Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application.
title_fullStr	Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application.
title_full_unstemmed	Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application.
title_sort	preparation of mouse monoclonal antibody for rb1cc1 and its clinical application.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a8176850734d4c13942308043e039a51
work_keys_str_mv	AT yusukehama preparationofmousemonoclonalantibodyforrb1cc1anditsclinicalapplication AT tokuhirochano preparationofmousemonoclonalantibodyforrb1cc1anditsclinicalapplication AT takumainui preparationofmousemonoclonalantibodyforrb1cc1anditsclinicalapplication AT kyoichimatsumoto preparationofmousemonoclonalantibodyforrb1cc1anditsclinicalapplication AT hidetoshiokabe preparationofmousemonoclonalantibodyforrb1cc1anditsclinicalapplication
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Preparation of mouse monoclonal antibody for RB1CC1 and its clinical application.

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