High performance micro-flow cytometer based on optical fibres

Abstract Flow cytometry is currently the gold standard for analysis of cells in the medical laboratory and biomedical research. Fuelled by the need of point-of-care diagnosis, a significant effort has been made to miniaturize and reduce cost of flow cytometers. However, despite recent advances, curr...

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Autores principales:	S. Etcheverry, A. Faridi, H. Ramachandraiah, T. Kumar, W. Margulis, F. Laurell, A. Russom
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a836c425014f43f3b2af442d9e72fc24
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spelling	oai:doaj.org-article:a836c425014f43f3b2af442d9e72fc242021-12-02T16:06:09ZHigh performance micro-flow cytometer based on optical fibres10.1038/s41598-017-05843-72045-2322https://doaj.org/article/a836c425014f43f3b2af442d9e72fc242017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05843-7https://doaj.org/toc/2045-2322Abstract Flow cytometry is currently the gold standard for analysis of cells in the medical laboratory and biomedical research. Fuelled by the need of point-of-care diagnosis, a significant effort has been made to miniaturize and reduce cost of flow cytometers. However, despite recent advances, current microsystems remain less versatile and much slower than their large-scale counterparts. In this work, an all-silica fibre microflow cytometer is presented that measures fluorescence and scattering from particles and cells. It integrates cell transport in circular capillaries and light delivery by optical fibres. Single-stream cell focusing is performed by Elasto-inertial microfluidics to guarantee accurate and sensitive detection. The capability of this technique is extended to high flow rates (up to 800 µl/min), enabling a throughput of 2500 particles/s. The robust, portable and low-cost system described here could be the basis for a point-of-care flow cytometer with a performance comparable to commercial systems.S. EtcheverryA. FaridiH. RamachandraiahT. KumarW. MargulisF. LaurellA. RussomNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. Etcheverry A. Faridi H. Ramachandraiah T. Kumar W. Margulis F. Laurell A. Russom High performance micro-flow cytometer based on optical fibres
description	Abstract Flow cytometry is currently the gold standard for analysis of cells in the medical laboratory and biomedical research. Fuelled by the need of point-of-care diagnosis, a significant effort has been made to miniaturize and reduce cost of flow cytometers. However, despite recent advances, current microsystems remain less versatile and much slower than their large-scale counterparts. In this work, an all-silica fibre microflow cytometer is presented that measures fluorescence and scattering from particles and cells. It integrates cell transport in circular capillaries and light delivery by optical fibres. Single-stream cell focusing is performed by Elasto-inertial microfluidics to guarantee accurate and sensitive detection. The capability of this technique is extended to high flow rates (up to 800 µl/min), enabling a throughput of 2500 particles/s. The robust, portable and low-cost system described here could be the basis for a point-of-care flow cytometer with a performance comparable to commercial systems.
format	article
author	S. Etcheverry A. Faridi H. Ramachandraiah T. Kumar W. Margulis F. Laurell A. Russom
author_facet	S. Etcheverry A. Faridi H. Ramachandraiah T. Kumar W. Margulis F. Laurell A. Russom
author_sort	S. Etcheverry
title	High performance micro-flow cytometer based on optical fibres
title_short	High performance micro-flow cytometer based on optical fibres
title_full	High performance micro-flow cytometer based on optical fibres
title_fullStr	High performance micro-flow cytometer based on optical fibres
title_full_unstemmed	High performance micro-flow cytometer based on optical fibres
title_sort	high performance micro-flow cytometer based on optical fibres
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a836c425014f43f3b2af442d9e72fc24
work_keys_str_mv	AT setcheverry highperformancemicroflowcytometerbasedonopticalfibres AT afaridi highperformancemicroflowcytometerbasedonopticalfibres AT hramachandraiah highperformancemicroflowcytometerbasedonopticalfibres AT tkumar highperformancemicroflowcytometerbasedonopticalfibres AT wmargulis highperformancemicroflowcytometerbasedonopticalfibres AT flaurell highperformancemicroflowcytometerbasedonopticalfibres AT arussom highperformancemicroflowcytometerbasedonopticalfibres
_version_	1718385108873904128

High performance micro-flow cytometer based on optical fibres

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