Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.

Adhesion of bacteria to mucus may favor their persistence within the gut and their beneficial effects to the host. Interactions between pig gastric mucin (PGM) and a natural isolate of Lactococcus lactis (TIL448) were measured at the single-cell scale and under static conditions, using atomic force...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Doan Thanh Lam Le, Thi-Ly Tran, Marie-Pierre Duviau, Mickael Meyrand, Yann Guérardel, Mickaël Castelain, Pascal Loubière, Marie-Pierre Chapot-Chartier, Etienne Dague, Muriel Mercier-Bonin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a836c6d4e1db4cd280bce465096ab8fe
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a836c6d4e1db4cd280bce465096ab8fe
record_format	dspace
spelling	oai:doaj.org-article:a836c6d4e1db4cd280bce465096ab8fe2021-11-18T08:45:57ZUnraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.1932-620310.1371/journal.pone.0079850https://doaj.org/article/a836c6d4e1db4cd280bce465096ab8fe2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24260308/?tool=EBIhttps://doaj.org/toc/1932-6203Adhesion of bacteria to mucus may favor their persistence within the gut and their beneficial effects to the host. Interactions between pig gastric mucin (PGM) and a natural isolate of Lactococcus lactis (TIL448) were measured at the single-cell scale and under static conditions, using atomic force microscopy (AFM). In parallel, these interactions were monitored at the bacterial population level and under shear flow. AFM experiments with a L. lactis cell-probe and a PGM-coated surface revealed a high proportion of specific adhesive events (60%) and a low level of non-adhesive ones (2%). The strain muco-adhesive properties were confirmed by the weak detachment of bacteria from the PGM-coated surface under shear flow. In AFM, rupture events were detected at short (100-200 nm) and long distances (up to 600-800 nm). AFM measurements on pili and mucus-binding protein defective mutants demonstrated the comparable role played by these two surface proteinaceous components in adhesion to PGM under static conditions. Under shear flow, a more important contribution of the mucus-binding protein than the pili one was observed. Both methods differ by the way of probing the adhesion force, i.e. negative force contact vs. sedimentation and normal-to-substratum retraction vs. tangential detachment conditions, using AFM and flow chamber, respectively. AFM blocking assays with free PGM or O-glycan fractions purified from PGM demonstrated that neutral oligosaccharides played a major role in adhesion of L. lactis TIL448 to PGM. This study dissects L. lactis muco-adhesive phenotype, in relation with the nature of the bacterial surface determinants.Doan Thanh Lam LeThi-Ly TranMarie-Pierre DuviauMickael MeyrandYann GuérardelMickaël CastelainPascal LoubièreMarie-Pierre Chapot-ChartierEtienne DagueMuriel Mercier-BoninPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79850 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Doan Thanh Lam Le Thi-Ly Tran Marie-Pierre Duviau Mickael Meyrand Yann Guérardel Mickaël Castelain Pascal Loubière Marie-Pierre Chapot-Chartier Etienne Dague Muriel Mercier-Bonin Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.
description	Adhesion of bacteria to mucus may favor their persistence within the gut and their beneficial effects to the host. Interactions between pig gastric mucin (PGM) and a natural isolate of Lactococcus lactis (TIL448) were measured at the single-cell scale and under static conditions, using atomic force microscopy (AFM). In parallel, these interactions were monitored at the bacterial population level and under shear flow. AFM experiments with a L. lactis cell-probe and a PGM-coated surface revealed a high proportion of specific adhesive events (60%) and a low level of non-adhesive ones (2%). The strain muco-adhesive properties were confirmed by the weak detachment of bacteria from the PGM-coated surface under shear flow. In AFM, rupture events were detected at short (100-200 nm) and long distances (up to 600-800 nm). AFM measurements on pili and mucus-binding protein defective mutants demonstrated the comparable role played by these two surface proteinaceous components in adhesion to PGM under static conditions. Under shear flow, a more important contribution of the mucus-binding protein than the pili one was observed. Both methods differ by the way of probing the adhesion force, i.e. negative force contact vs. sedimentation and normal-to-substratum retraction vs. tangential detachment conditions, using AFM and flow chamber, respectively. AFM blocking assays with free PGM or O-glycan fractions purified from PGM demonstrated that neutral oligosaccharides played a major role in adhesion of L. lactis TIL448 to PGM. This study dissects L. lactis muco-adhesive phenotype, in relation with the nature of the bacterial surface determinants.
format	article
author	Doan Thanh Lam Le Thi-Ly Tran Marie-Pierre Duviau Mickael Meyrand Yann Guérardel Mickaël Castelain Pascal Loubière Marie-Pierre Chapot-Chartier Etienne Dague Muriel Mercier-Bonin
author_facet	Doan Thanh Lam Le Thi-Ly Tran Marie-Pierre Duviau Mickael Meyrand Yann Guérardel Mickaël Castelain Pascal Loubière Marie-Pierre Chapot-Chartier Etienne Dague Muriel Mercier-Bonin
author_sort	Doan Thanh Lam Le
title	Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.
title_short	Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.
title_full	Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.
title_fullStr	Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.
title_full_unstemmed	Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.
title_sort	unraveling the role of surface mucus-binding protein and pili in muco-adhesion of lactococcus lactis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a836c6d4e1db4cd280bce465096ab8fe
work_keys_str_mv	AT doanthanhlamle unravelingtheroleofsurfacemucusbindingproteinandpiliinmucoadhesionoflactococcuslactis AT thilytran unravelingtheroleofsurfacemucusbindingproteinandpiliinmucoadhesionoflactococcuslactis AT mariepierreduviau unravelingtheroleofsurfacemucusbindingproteinandpiliinmucoadhesionoflactococcuslactis AT mickaelmeyrand unravelingtheroleofsurfacemucusbindingproteinandpiliinmucoadhesionoflactococcuslactis AT yannguerardel unravelingtheroleofsurfacemucusbindingproteinandpiliinmucoadhesionoflactococcuslactis AT mickaelcastelain unravelingtheroleofsurfacemucusbindingproteinandpiliinmucoadhesionoflactococcuslactis AT pascalloubiere unravelingtheroleofsurfacemucusbindingproteinandpiliinmucoadhesionoflactococcuslactis AT mariepierrechapotchartier unravelingtheroleofsurfacemucusbindingproteinandpiliinmucoadhesionoflactococcuslactis AT etiennedague unravelingtheroleofsurfacemucusbindingproteinandpiliinmucoadhesionoflactococcuslactis AT murielmercierbonin unravelingtheroleofsurfacemucusbindingproteinandpiliinmucoadhesionoflactococcuslactis
_version_	1718421341296656384

Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.

Ejemplares similares