Engineered yeast genomes accurately assembled from pure and mixed samples

The cost and complexity of whole genome sequencing limits its use in identifying and validating sequences used for genetic engineering and synthetic biology. Here the authors present Prymetime, an integrated workflow to sequence engineered strains and identify engineering in metagenomes.

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Autores principales:	Joseph H. Collins, Kevin W. Keating, Trent R. Jones, Shravani Balaji, Celeste B. Marsan, Marina Çomo, Zachary J. Newlon, Tom Mitchell, Bryan Bartley, Aaron Adler, Nicholas Roehner, Eric M. Young
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a84252f6c5bb479cb7fcefe72ee9704b
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spelling	oai:doaj.org-article:a84252f6c5bb479cb7fcefe72ee9704b2021-12-02T13:32:54ZEngineered yeast genomes accurately assembled from pure and mixed samples10.1038/s41467-021-21656-92041-1723https://doaj.org/article/a84252f6c5bb479cb7fcefe72ee9704b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21656-9https://doaj.org/toc/2041-1723The cost and complexity of whole genome sequencing limits its use in identifying and validating sequences used for genetic engineering and synthetic biology. Here the authors present Prymetime, an integrated workflow to sequence engineered strains and identify engineering in metagenomes.Joseph H. CollinsKevin W. KeatingTrent R. JonesShravani BalajiCeleste B. MarsanMarina ÇomoZachary J. NewlonTom MitchellBryan BartleyAaron AdlerNicholas RoehnerEric M. YoungNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Joseph H. Collins Kevin W. Keating Trent R. Jones Shravani Balaji Celeste B. Marsan Marina Çomo Zachary J. Newlon Tom Mitchell Bryan Bartley Aaron Adler Nicholas Roehner Eric M. Young Engineered yeast genomes accurately assembled from pure and mixed samples
description	The cost and complexity of whole genome sequencing limits its use in identifying and validating sequences used for genetic engineering and synthetic biology. Here the authors present Prymetime, an integrated workflow to sequence engineered strains and identify engineering in metagenomes.
format	article
author	Joseph H. Collins Kevin W. Keating Trent R. Jones Shravani Balaji Celeste B. Marsan Marina Çomo Zachary J. Newlon Tom Mitchell Bryan Bartley Aaron Adler Nicholas Roehner Eric M. Young
author_facet	Joseph H. Collins Kevin W. Keating Trent R. Jones Shravani Balaji Celeste B. Marsan Marina Çomo Zachary J. Newlon Tom Mitchell Bryan Bartley Aaron Adler Nicholas Roehner Eric M. Young
author_sort	Joseph H. Collins
title	Engineered yeast genomes accurately assembled from pure and mixed samples
title_short	Engineered yeast genomes accurately assembled from pure and mixed samples
title_full	Engineered yeast genomes accurately assembled from pure and mixed samples
title_fullStr	Engineered yeast genomes accurately assembled from pure and mixed samples
title_full_unstemmed	Engineered yeast genomes accurately assembled from pure and mixed samples
title_sort	engineered yeast genomes accurately assembled from pure and mixed samples
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a84252f6c5bb479cb7fcefe72ee9704b
work_keys_str_mv	AT josephhcollins engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples AT kevinwkeating engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples AT trentrjones engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples AT shravanibalaji engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples AT celestebmarsan engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples AT marinacomo engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples AT zacharyjnewlon engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples AT tommitchell engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples AT bryanbartley engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples AT aaronadler engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples AT nicholasroehner engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples AT ericmyoung engineeredyeastgenomesaccuratelyassembledfrompureandmixedsamples
_version_	1718392873447063552

Engineered yeast genomes accurately assembled from pure and mixed samples

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