Peran Brush Border Membrane Vesicle (BBMV) dalam proses infeksi Baculovirus

The Role of Brush Border Membrane Vesicle (BBMV) in The Baculovirus Infection. Although baculovirus becoming recognized as potential important agents for control pests, there are several important obstacles for commercial use insecticide. On...

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Autor principal:	Arman Wijonarko
Formato:	article
Lenguaje:	EN ID
Publicado:	The Entomological Society of Indonesia 2017
Materias:	BBMV, baculovirus, infection. Zoology QL1-991
Acceso en línea:	https://doaj.org/article/a84bcadbd5c44c20a835cc22b4be6dac
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spelling	oai:doaj.org-article:a84bcadbd5c44c20a835cc22b4be6dac2021-12-02T04:28:06ZPeran Brush Border Membrane Vesicle (BBMV) dalam proses infeksi Baculovirus1829-77222089-025710.5994/jei.2.2.8https://doaj.org/article/a84bcadbd5c44c20a835cc22b4be6dac2017-02-01T00:00:00Zhttp://jurnal.pei-pusat.org/index.php/jei/article/view/292https://doaj.org/toc/1829-7722https://doaj.org/toc/2089-0257<p><strong>The Role of <em>Brush Border Membrane Vesicle</em> (BBMV) in The Baculovirus Infection.</strong> Although baculovirus becoming recognized as potential important agents for control pests, there are several important obstacles for commercial use insecticide. One important obstacle is their narrow host. The other obstacle is due to its development resistance, the phenomenon whereby host larvae become progressively more resistant to fatal infection as they age within and among instars. From practical stand point, this phenomenon impacts the effectiveness of baculovirus control programs against agricultural and forest insect pests that is necessary to adjust application levels in the response to the demography of the target insect population. Purified columnar cells derived from midgut of armyworm <em>Pseudaletia separata </em>was used for fusion assay and observed by means of fluorescence assay based on self-quenching of octadecylrhodamine B chloride (R18). Confocal microscopy of the columnar cells that had been combined with labeled virus showed that the fluorescence appeared on the apical part in the brush border membrane, this indicated that there was fusion between virus and the cells. Two dimensional SDS-PAGE and immunobloting assay confirmed that the receptor exist within the columnar cells.</p>Arman WijonarkoThe Entomological Society of IndonesiaarticleBBMV, baculovirus, infection.ZoologyQL1-991ENIDJurnal Entomologi Indonesia, Vol 2, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	BBMV, baculovirus, infection. Zoology QL1-991
spellingShingle	BBMV, baculovirus, infection. Zoology QL1-991 Arman Wijonarko Peran Brush Border Membrane Vesicle (BBMV) dalam proses infeksi Baculovirus
description	<p><strong>The Role of <em>Brush Border Membrane Vesicle</em> (BBMV) in The Baculovirus Infection.</strong> Although baculovirus becoming recognized as potential important agents for control pests, there are several important obstacles for commercial use insecticide. One important obstacle is their narrow host. The other obstacle is due to its development resistance, the phenomenon whereby host larvae become progressively more resistant to fatal infection as they age within and among instars. From practical stand point, this phenomenon impacts the effectiveness of baculovirus control programs against agricultural and forest insect pests that is necessary to adjust application levels in the response to the demography of the target insect population. Purified columnar cells derived from midgut of armyworm <em>Pseudaletia separata </em>was used for fusion assay and observed by means of fluorescence assay based on self-quenching of octadecylrhodamine B chloride (R18). Confocal microscopy of the columnar cells that had been combined with labeled virus showed that the fluorescence appeared on the apical part in the brush border membrane, this indicated that there was fusion between virus and the cells. Two dimensional SDS-PAGE and immunobloting assay confirmed that the receptor exist within the columnar cells.</p>
format	article
author	Arman Wijonarko
author_facet	Arman Wijonarko
author_sort	Arman Wijonarko
title	Peran Brush Border Membrane Vesicle (BBMV) dalam proses infeksi Baculovirus
title_short	Peran Brush Border Membrane Vesicle (BBMV) dalam proses infeksi Baculovirus
title_full	Peran Brush Border Membrane Vesicle (BBMV) dalam proses infeksi Baculovirus
title_fullStr	Peran Brush Border Membrane Vesicle (BBMV) dalam proses infeksi Baculovirus
title_full_unstemmed	Peran Brush Border Membrane Vesicle (BBMV) dalam proses infeksi Baculovirus
title_sort	peran brush border membrane vesicle (bbmv) dalam proses infeksi baculovirus
publisher	The Entomological Society of Indonesia
publishDate	2017
url	https://doaj.org/article/a84bcadbd5c44c20a835cc22b4be6dac
work_keys_str_mv	AT armanwijonarko peranbrushbordermembranevesiclebbmvdalamprosesinfeksibaculovirus
_version_	1718401208461295616

Peran Brush Border Membrane Vesicle (BBMV) dalam proses infeksi Baculovirus

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