Mannosylated liposomes for targeted gene delivery
Fansheng Kong1, Fang Zhou1, Linfu Ge1, Ximin Liu1, Yong Wang21Department of Hematology, 2Department of Rehabilitation and Physiotherapy, General Hospital of Ji'nan Command, PLA, Ji'nan, People's Republic of ChinaBackground: Liposomes can be modified with different...
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Dove Medical Press
2012
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oai:doaj.org-article:a84cc3ac546a465e823490fd0c6c6fab2021-12-02T02:29:50ZMannosylated liposomes for targeted gene delivery1176-91141178-2013https://doaj.org/article/a84cc3ac546a465e823490fd0c6c6fab2012-02-01T00:00:00Zhttp://www.dovepress.com/mannosylated-liposomes-for-targeted-gene-delivery-a9320https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Fansheng Kong1, Fang Zhou1, Linfu Ge1, Ximin Liu1, Yong Wang21Department of Hematology, 2Department of Rehabilitation and Physiotherapy, General Hospital of Ji'nan Command, PLA, Ji'nan, People's Republic of ChinaBackground: Liposomes can be modified with different ligands to control their biological properties, such as longevity, targeting ability, and intracellular penetration, in a desired fashion. The aim of this study was to modify liposomes with a novel mannosylated polyethylene glycol-phosphatidylethanolamine (M-PEG-PE) ligand to achieve active targeted gene delivery.Methods: Rat Kupffer cells were isolated and used as model cells for in vitro evaluation of cytotoxicity and transfection efficiency. The modified liposomes were intravenously injected into the rats, and Kupffer cells were isolated and analyzed by flow cytometry for in vivo gene delivery and expression.Results: The M-PEG-PE-modified liposome-enhanced green fluorescence protein plasmid (M-PEG-PE-Lipo-pEGFP) complexes had a particle size of 237 nm and a loading efficiency of 90%. The M-PEG-PE-Lipo-pEGFP complexes displayed remarkably higher transfection efficiency than unmodified Lipo-pEGFP, both in vitro (51%–30%) and in vivo (43%–27%).Conclusion: M-PEG-PE could function as an excellent active targeting ligand, and M-PEG-PE-modified liposomes could be promising active targeted drug delivery vectors.Keywords: gene delivery, active targeting, mannosylated, polyethylene glycol, phosphatidylethanolamine, liposomesKong FZhou FGe LLiu XWang Y.Dove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1079-1089 (2012) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Medicine (General) R5-920 |
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Medicine (General) R5-920 Kong F Zhou F Ge L Liu X Wang Y. Mannosylated liposomes for targeted gene delivery |
| description |
Fansheng Kong1, Fang Zhou1, Linfu Ge1, Ximin Liu1, Yong Wang21Department of Hematology, 2Department of Rehabilitation and Physiotherapy, General Hospital of Ji'nan Command, PLA, Ji'nan, People's Republic of ChinaBackground: Liposomes can be modified with different ligands to control their biological properties, such as longevity, targeting ability, and intracellular penetration, in a desired fashion. The aim of this study was to modify liposomes with a novel mannosylated polyethylene glycol-phosphatidylethanolamine (M-PEG-PE) ligand to achieve active targeted gene delivery.Methods: Rat Kupffer cells were isolated and used as model cells for in vitro evaluation of cytotoxicity and transfection efficiency. The modified liposomes were intravenously injected into the rats, and Kupffer cells were isolated and analyzed by flow cytometry for in vivo gene delivery and expression.Results: The M-PEG-PE-modified liposome-enhanced green fluorescence protein plasmid (M-PEG-PE-Lipo-pEGFP) complexes had a particle size of 237 nm and a loading efficiency of 90%. The M-PEG-PE-Lipo-pEGFP complexes displayed remarkably higher transfection efficiency than unmodified Lipo-pEGFP, both in vitro (51%–30%) and in vivo (43%–27%).Conclusion: M-PEG-PE could function as an excellent active targeting ligand, and M-PEG-PE-modified liposomes could be promising active targeted drug delivery vectors.Keywords: gene delivery, active targeting, mannosylated, polyethylene glycol, phosphatidylethanolamine, liposomes |
| format |
article |
| author |
Kong F Zhou F Ge L Liu X Wang Y. |
| author_facet |
Kong F Zhou F Ge L Liu X Wang Y. |
| author_sort |
Kong F |
| title |
Mannosylated liposomes for targeted gene delivery |
| title_short |
Mannosylated liposomes for targeted gene delivery |
| title_full |
Mannosylated liposomes for targeted gene delivery |
| title_fullStr |
Mannosylated liposomes for targeted gene delivery |
| title_full_unstemmed |
Mannosylated liposomes for targeted gene delivery |
| title_sort |
mannosylated liposomes for targeted gene delivery |
| publisher |
Dove Medical Press |
| publishDate |
2012 |
| url |
https://doaj.org/article/a84cc3ac546a465e823490fd0c6c6fab |
| work_keys_str_mv |
AT kongf mannosylatedliposomesfortargetedgenedelivery AT zhouf mannosylatedliposomesfortargetedgenedelivery AT gel mannosylatedliposomesfortargetedgenedelivery AT liux mannosylatedliposomesfortargetedgenedelivery AT wangy mannosylatedliposomesfortargetedgenedelivery |
| _version_ |
1718402429782851584 |