Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons

Healing of ruptured tendons remains a clinical challenge because of its slow progress and relatively weak mechanical force at an early stage. Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have therapeutic potential for tissue regeneration. In this study, we isolated EVs fro...

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Autores principales:	Shih-Heng Chen, Zhi-Yu Chen, Ya-Hsuan Lin, Shih-Hsien Chen, Pang-Yun Chou, Huang-Kai Kao, Feng-Huei Lin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adipose-derived stem cells extracellular vesicles tendon healing tendon regeneration Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/a87205de94284d6bae81f04ac95274e9
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spelling	oai:doaj.org-article:a87205de94284d6bae81f04ac95274e92021-11-25T17:55:51ZExtracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons10.3390/ijms2222123731422-00671661-6596https://doaj.org/article/a87205de94284d6bae81f04ac95274e92021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12373https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Healing of ruptured tendons remains a clinical challenge because of its slow progress and relatively weak mechanical force at an early stage. Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have therapeutic potential for tissue regeneration. In this study, we isolated EVs from adipose-derived stem cells (ADSCs) and evaluated their ability to promote tendon regeneration. Our results indicated that ADSC-EVs significantly enhanced the proliferation and migration of tenocytes in vitro. To further study the roles of ADSC-EVs in tendon regeneration, ADSC-EVs were used in Achilles tendon repair in rabbits. The mechanical strength, histology, and protein expression in the injured tendon tissues significantly improved 4 weeks after ADSC-EV treatment. Decorin and biglycan were significantly upregulated in comparison to the untreated controls. In summary, ADSC-EVs stimulated the proliferation and migration of tenocytes and improved the mechanical strength of repaired tendons, suggesting that ADSC-EV treatment is a potential highly potent therapeutic strategy for tendon injuries.Shih-Heng ChenZhi-Yu ChenYa-Hsuan LinShih-Hsien ChenPang-Yun ChouHuang-Kai KaoFeng-Huei LinMDPI AGarticleadipose-derived stem cellsextracellular vesiclestendon healingtendon regenerationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12373, p 12373 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipose-derived stem cells extracellular vesicles tendon healing tendon regeneration Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	adipose-derived stem cells extracellular vesicles tendon healing tendon regeneration Biology (General) QH301-705.5 Chemistry QD1-999 Shih-Heng Chen Zhi-Yu Chen Ya-Hsuan Lin Shih-Hsien Chen Pang-Yun Chou Huang-Kai Kao Feng-Huei Lin Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons
description	Healing of ruptured tendons remains a clinical challenge because of its slow progress and relatively weak mechanical force at an early stage. Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have therapeutic potential for tissue regeneration. In this study, we isolated EVs from adipose-derived stem cells (ADSCs) and evaluated their ability to promote tendon regeneration. Our results indicated that ADSC-EVs significantly enhanced the proliferation and migration of tenocytes in vitro. To further study the roles of ADSC-EVs in tendon regeneration, ADSC-EVs were used in Achilles tendon repair in rabbits. The mechanical strength, histology, and protein expression in the injured tendon tissues significantly improved 4 weeks after ADSC-EV treatment. Decorin and biglycan were significantly upregulated in comparison to the untreated controls. In summary, ADSC-EVs stimulated the proliferation and migration of tenocytes and improved the mechanical strength of repaired tendons, suggesting that ADSC-EV treatment is a potential highly potent therapeutic strategy for tendon injuries.
format	article
author	Shih-Heng Chen Zhi-Yu Chen Ya-Hsuan Lin Shih-Hsien Chen Pang-Yun Chou Huang-Kai Kao Feng-Huei Lin
author_facet	Shih-Heng Chen Zhi-Yu Chen Ya-Hsuan Lin Shih-Hsien Chen Pang-Yun Chou Huang-Kai Kao Feng-Huei Lin
author_sort	Shih-Heng Chen
title	Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons
title_short	Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons
title_full	Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons
title_fullStr	Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons
title_full_unstemmed	Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons
title_sort	extracellular vesicles of adipose-derived stem cells promote the healing of traumatized achilles tendons
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a87205de94284d6bae81f04ac95274e9
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Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons

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