Improved Interchangeability with Different Corneal Specular Microscopes for Quantitative Endothelial Cell Analysis

Gwyneth A van Rijn, 1 C Jasper F Wijnen, 1 Bart TH van Dooren, 2, 3 Yanny YY Cheng, 1 Jan-Willem M Beenakker, 1, 4 Gregorius PM Luyten 1 1Department of Ophthalmology, Leiden University Medical Center, Leiden, The Netherlands; 2Department of Ophthalmology, Erasmus Medical Center, Rotterdam, The Net...

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Autores principales:	van Rijn GA, Wijnen CJF, van Dooren BTH, Cheng YYY, Beenakker JWM, Luyten GPM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	corneal endothelium corneal endothelial cells specular microscopy endothelial cell count endothelial cell density corneal endothelial cell analysis phakic intraocular lens Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/a87eff0caa374bb68b76133da9a8a7b2
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spelling	oai:doaj.org-article:a87eff0caa374bb68b76133da9a8a7b22021-12-02T04:35:03ZImproved Interchangeability with Different Corneal Specular Microscopes for Quantitative Endothelial Cell Analysis1177-5483https://doaj.org/article/a87eff0caa374bb68b76133da9a8a7b22020-01-01T00:00:00Zhttps://www.dovepress.com/improved-interchangeability-with-different-corneal-specular-microscope-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Gwyneth A van Rijn, 1 C Jasper F Wijnen, 1 Bart TH van Dooren, 2, 3 Yanny YY Cheng, 1 Jan-Willem M Beenakker, 1, 4 Gregorius PM Luyten 1 1Department of Ophthalmology, Leiden University Medical Center, Leiden, The Netherlands; 2Department of Ophthalmology, Erasmus Medical Center, Rotterdam, The Netherlands; 3Department of Ophthalmology, Amphia Medical Hospital, Breda, The Netherlands; 4C.J.Gorter Center for High Field MRI, Department of Radiology, Leiden University Medical Center, Leiden, The NetherlandsCorrespondence: Gwyneth A van RijnDepartment of Ophthalmology, Leiden University Medical Center, Leiden, The NetherlandsEmail g.a.van_rijn@lumc.nlIntroduction: During our clinical practice and research, we encountered an interchangeability problem when using the SP-2000P and SP-3000P TopCon corneal specular microscopes (CSMs) (TopCon Medical Systems, Tokyo, Japan) regarding the endothelial cell count (ECC). We describe a method to improve interchangeability between these CSMs.Methods: Five consecutive good-quality endothelial cell photographs were obtained in 22 eyes of 11 subjects. An ECC comparison between the two CSMs was performed after (I) gauging and calibration by the manufacturer, (II) adjustment of the magnification, (III) correction after external horizontal and vertical calibration.Results: There was a statistically significant difference between the ECC of the SP-2000P and SP-3000P at the start. The SP-2000P counted an average of 500 cells/mm 2 more than the SP-3000P (p=0.00). After correction for magnification and determining a correction factor based on external calibration, the difference between the ECC of the SP-2000P and the SP-3000P was then found to be 0.4 cells/mm 2 and was not statistically significant (p=0.98).Discussion: We propose a method for improving interchangeability, which involves checking magnification settings, re-checking magnification calibration with external calibration devices, and then calculating correction factors. This method can be applied to various specular or confocal microscopes and their associated endothelial cell analysis software packages to be able to keep performing precise endothelial cell counts and to enable comparison of ECCs when a CSM needs to be replaced or when results from different microscopes need to be compared.Keywords: corneal endothelium, corneal endothelial cells, specular microscopy, endothelial cell count, endothelial cell density, corneal endothelial cell analysisvan Rijn GAWijnen CJFvan Dooren BTHCheng YYYBeenakker JWMLuyten GPMDove Medical Pressarticlecorneal endotheliumcorneal endothelial cellsspecular microscopyendothelial cell countendothelial cell densitycorneal endothelial cell analysisphakic intraocular lensOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 14, Pp 61-70 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	corneal endothelium corneal endothelial cells specular microscopy endothelial cell count endothelial cell density corneal endothelial cell analysis phakic intraocular lens Ophthalmology RE1-994
spellingShingle	corneal endothelium corneal endothelial cells specular microscopy endothelial cell count endothelial cell density corneal endothelial cell analysis phakic intraocular lens Ophthalmology RE1-994 van Rijn GA Wijnen CJF van Dooren BTH Cheng YYY Beenakker JWM Luyten GPM Improved Interchangeability with Different Corneal Specular Microscopes for Quantitative Endothelial Cell Analysis
description	Gwyneth A van Rijn, 1 C Jasper F Wijnen, 1 Bart TH van Dooren, 2, 3 Yanny YY Cheng, 1 Jan-Willem M Beenakker, 1, 4 Gregorius PM Luyten 1 1Department of Ophthalmology, Leiden University Medical Center, Leiden, The Netherlands; 2Department of Ophthalmology, Erasmus Medical Center, Rotterdam, The Netherlands; 3Department of Ophthalmology, Amphia Medical Hospital, Breda, The Netherlands; 4C.J.Gorter Center for High Field MRI, Department of Radiology, Leiden University Medical Center, Leiden, The NetherlandsCorrespondence: Gwyneth A van RijnDepartment of Ophthalmology, Leiden University Medical Center, Leiden, The NetherlandsEmail g.a.van_rijn@lumc.nlIntroduction: During our clinical practice and research, we encountered an interchangeability problem when using the SP-2000P and SP-3000P TopCon corneal specular microscopes (CSMs) (TopCon Medical Systems, Tokyo, Japan) regarding the endothelial cell count (ECC). We describe a method to improve interchangeability between these CSMs.Methods: Five consecutive good-quality endothelial cell photographs were obtained in 22 eyes of 11 subjects. An ECC comparison between the two CSMs was performed after (I) gauging and calibration by the manufacturer, (II) adjustment of the magnification, (III) correction after external horizontal and vertical calibration.Results: There was a statistically significant difference between the ECC of the SP-2000P and SP-3000P at the start. The SP-2000P counted an average of 500 cells/mm 2 more than the SP-3000P (p=0.00). After correction for magnification and determining a correction factor based on external calibration, the difference between the ECC of the SP-2000P and the SP-3000P was then found to be 0.4 cells/mm 2 and was not statistically significant (p=0.98).Discussion: We propose a method for improving interchangeability, which involves checking magnification settings, re-checking magnification calibration with external calibration devices, and then calculating correction factors. This method can be applied to various specular or confocal microscopes and their associated endothelial cell analysis software packages to be able to keep performing precise endothelial cell counts and to enable comparison of ECCs when a CSM needs to be replaced or when results from different microscopes need to be compared.Keywords: corneal endothelium, corneal endothelial cells, specular microscopy, endothelial cell count, endothelial cell density, corneal endothelial cell analysis
format	article
author	van Rijn GA Wijnen CJF van Dooren BTH Cheng YYY Beenakker JWM Luyten GPM
author_facet	van Rijn GA Wijnen CJF van Dooren BTH Cheng YYY Beenakker JWM Luyten GPM
author_sort	van Rijn GA
title	Improved Interchangeability with Different Corneal Specular Microscopes for Quantitative Endothelial Cell Analysis
title_short	Improved Interchangeability with Different Corneal Specular Microscopes for Quantitative Endothelial Cell Analysis
title_full	Improved Interchangeability with Different Corneal Specular Microscopes for Quantitative Endothelial Cell Analysis
title_fullStr	Improved Interchangeability with Different Corneal Specular Microscopes for Quantitative Endothelial Cell Analysis
title_full_unstemmed	Improved Interchangeability with Different Corneal Specular Microscopes for Quantitative Endothelial Cell Analysis
title_sort	improved interchangeability with different corneal specular microscopes for quantitative endothelial cell analysis
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/a87eff0caa374bb68b76133da9a8a7b2
work_keys_str_mv	AT vanrijnga improvedinterchangeabilitywithdifferentcornealspecularmicroscopesforquantitativeendothelialcellanalysis AT wijnencjf improvedinterchangeabilitywithdifferentcornealspecularmicroscopesforquantitativeendothelialcellanalysis AT vandoorenbth improvedinterchangeabilitywithdifferentcornealspecularmicroscopesforquantitativeendothelialcellanalysis AT chengyyy improvedinterchangeabilitywithdifferentcornealspecularmicroscopesforquantitativeendothelialcellanalysis AT beenakkerjwm improvedinterchangeabilitywithdifferentcornealspecularmicroscopesforquantitativeendothelialcellanalysis AT luytengpm improvedinterchangeabilitywithdifferentcornealspecularmicroscopesforquantitativeendothelialcellanalysis
_version_	1718401142797369344

Improved Interchangeability with Different Corneal Specular Microscopes for Quantitative Endothelial Cell Analysis

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