Antitumor effect of hyaluronic-acid-modified chitosan nanoparticles loaded with siRNA for targeted therapy for non-small cell lung cancer

Wenhua Zhang,1,2,* Wenhua Xu,1,* Yu Lan,3 Xuliang He,1 Kaibin Liu,4 Ye Liang21Department of Inspection, Medical Faculty, Qingdao University, Qingdao 266003, People’s Republic of China; 2Key Laboratory, Department of Urology and Andrology, Affiliated Hospital of Qingdao University,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang W, Xu W, Lan Y, He X, Liu K, Liang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	nanoparticle siRNA CD44 hyaluronic acid cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a8858cc1d5614f82a25fcec081abb741
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a8858cc1d5614f82a25fcec081abb741
record_format	dspace
spelling	oai:doaj.org-article:a8858cc1d5614f82a25fcec081abb7412021-12-02T03:06:29ZAntitumor effect of hyaluronic-acid-modified chitosan nanoparticles loaded with siRNA for targeted therapy for non-small cell lung cancer1178-2013https://doaj.org/article/a8858cc1d5614f82a25fcec081abb7412019-07-01T00:00:00Zhttps://www.dovepress.com/antitumor-effect-of-hyaluronic-acid-modified-chitosan-nanoparticles-lo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wenhua Zhang,1,2,* Wenhua Xu,1,* Yu Lan,3 Xuliang He,1 Kaibin Liu,4 Ye Liang21Department of Inspection, Medical Faculty, Qingdao University, Qingdao 266003, People’s Republic of China; 2Key Laboratory, Department of Urology and Andrology, Affiliated Hospital of Qingdao University, Qingdao 266003, People’s Republic of China; 3Department of Inspection, Weihai Central Hospital, Weihai 264400, People’s Republic of China; 4Department of Clinical Medicine, Second Military Medical University, Shanghai 200433, People’s Republic of China*These authors contributed equally to this workPurpose: Nanoparticle (NP)-mediated targeted delivery of therapeutic genes or siRNAs to tumors has potential advantages. In this study, hyaluronic acid (HA)-modified chitosan nanoparticles (CS NPs-HA) loaded with cyanine 3 (Cy3)-labeled siRNA (sCS NPs-HA) were prepared and characterized.Methods: Human non-small cell lung cancer (NSCLC) A549 cells expressing receptor CD44 and tumor-bearing mice were used to evaluate the cytotoxic and antitumor effects of sCS NPs-HA in vitro and in vivo.Results: The results showed that noncytotoxic CS NPs-HA of small size (100–200 nm) effectively delivered the Cy3-labeled siRNA to A549 cells via receptor CD44 and inhibited cell proliferation by downregulating the target gene BCL2. In vivo experiment results revealed that sCS NPs-HA directly delivered greater amounts of Cy3-labeled siRNA to the tumor sites, resulting in the inhibition of tumor growth by downregulating BCL2, as compared to unmodified NPs loaded with siRNA (sCS NPs) and to naked Cy3-labeled siRNA.Conclusion: The HA-modified NPs based on chitosan could serve as a promising carrier for siRNA delivery and targeted therapy for NSCLC expressing CD44.Keywords: nanoparticle, siRNA, CD44, hyaluronic acid, cancerZhang WXu WLan YHe XLiu KLiang YDove Medical PressarticlenanoparticlesiRNACD44hyaluronic acidcancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 5287-5301 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoparticle siRNA CD44 hyaluronic acid cancer Medicine (General) R5-920
spellingShingle	nanoparticle siRNA CD44 hyaluronic acid cancer Medicine (General) R5-920 Zhang W Xu W Lan Y He X Liu K Liang Y Antitumor effect of hyaluronic-acid-modified chitosan nanoparticles loaded with siRNA for targeted therapy for non-small cell lung cancer
description	Wenhua Zhang,1,2,* Wenhua Xu,1,* Yu Lan,3 Xuliang He,1 Kaibin Liu,4 Ye Liang21Department of Inspection, Medical Faculty, Qingdao University, Qingdao 266003, People’s Republic of China; 2Key Laboratory, Department of Urology and Andrology, Affiliated Hospital of Qingdao University, Qingdao 266003, People’s Republic of China; 3Department of Inspection, Weihai Central Hospital, Weihai 264400, People’s Republic of China; 4Department of Clinical Medicine, Second Military Medical University, Shanghai 200433, People’s Republic of China*These authors contributed equally to this workPurpose: Nanoparticle (NP)-mediated targeted delivery of therapeutic genes or siRNAs to tumors has potential advantages. In this study, hyaluronic acid (HA)-modified chitosan nanoparticles (CS NPs-HA) loaded with cyanine 3 (Cy3)-labeled siRNA (sCS NPs-HA) were prepared and characterized.Methods: Human non-small cell lung cancer (NSCLC) A549 cells expressing receptor CD44 and tumor-bearing mice were used to evaluate the cytotoxic and antitumor effects of sCS NPs-HA in vitro and in vivo.Results: The results showed that noncytotoxic CS NPs-HA of small size (100–200 nm) effectively delivered the Cy3-labeled siRNA to A549 cells via receptor CD44 and inhibited cell proliferation by downregulating the target gene BCL2. In vivo experiment results revealed that sCS NPs-HA directly delivered greater amounts of Cy3-labeled siRNA to the tumor sites, resulting in the inhibition of tumor growth by downregulating BCL2, as compared to unmodified NPs loaded with siRNA (sCS NPs) and to naked Cy3-labeled siRNA.Conclusion: The HA-modified NPs based on chitosan could serve as a promising carrier for siRNA delivery and targeted therapy for NSCLC expressing CD44.Keywords: nanoparticle, siRNA, CD44, hyaluronic acid, cancer
format	article
author	Zhang W Xu W Lan Y He X Liu K Liang Y
author_facet	Zhang W Xu W Lan Y He X Liu K Liang Y
author_sort	Zhang W
title	Antitumor effect of hyaluronic-acid-modified chitosan nanoparticles loaded with siRNA for targeted therapy for non-small cell lung cancer
title_short	Antitumor effect of hyaluronic-acid-modified chitosan nanoparticles loaded with siRNA for targeted therapy for non-small cell lung cancer
title_full	Antitumor effect of hyaluronic-acid-modified chitosan nanoparticles loaded with siRNA for targeted therapy for non-small cell lung cancer
title_fullStr	Antitumor effect of hyaluronic-acid-modified chitosan nanoparticles loaded with siRNA for targeted therapy for non-small cell lung cancer
title_full_unstemmed	Antitumor effect of hyaluronic-acid-modified chitosan nanoparticles loaded with siRNA for targeted therapy for non-small cell lung cancer
title_sort	antitumor effect of hyaluronic-acid-modified chitosan nanoparticles loaded with sirna for targeted therapy for non-small cell lung cancer
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/a8858cc1d5614f82a25fcec081abb741
work_keys_str_mv	AT zhangw antitumoreffectofhyaluronicacidmodifiedchitosannanoparticlesloadedwithsirnafortargetedtherapyfornonsmallcelllungcancer AT xuw antitumoreffectofhyaluronicacidmodifiedchitosannanoparticlesloadedwithsirnafortargetedtherapyfornonsmallcelllungcancer AT lany antitumoreffectofhyaluronicacidmodifiedchitosannanoparticlesloadedwithsirnafortargetedtherapyfornonsmallcelllungcancer AT hex antitumoreffectofhyaluronicacidmodifiedchitosannanoparticlesloadedwithsirnafortargetedtherapyfornonsmallcelllungcancer AT liuk antitumoreffectofhyaluronicacidmodifiedchitosannanoparticlesloadedwithsirnafortargetedtherapyfornonsmallcelllungcancer AT liangy antitumoreffectofhyaluronicacidmodifiedchitosannanoparticlesloadedwithsirnafortargetedtherapyfornonsmallcelllungcancer
_version_	1718401938984271872

Antitumor effect of hyaluronic-acid-modified chitosan nanoparticles loaded with siRNA for targeted therapy for non-small cell lung cancer

Ejemplares similares