The roles of history, chance, and natural selection in the evolution of antibiotic resistance

History, chance, and selection are the fundamental factors that drive and constrain evolution. We designed evolution experiments to disentangle and quantify effects of these forces on the evolution of antibiotic resistance. Previously, we showed that selection of the pathogen Acinetobacter baumannii...

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Autores principales:	Alfonso Santos-Lopez, Christopher W Marshall, Allison L Haas, Caroline Turner, Javier Rasero, Vaughn S Cooper
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	acinetobacter collateral sensitivity population genetics genomics efflux Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a885931ec4fa4f99b3d2d8a163af2398
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spelling	oai:doaj.org-article:a885931ec4fa4f99b3d2d8a163af23982021-12-01T12:14:12ZThe roles of history, chance, and natural selection in the evolution of antibiotic resistance10.7554/eLife.706762050-084Xe70676https://doaj.org/article/a885931ec4fa4f99b3d2d8a163af23982021-08-01T00:00:00Zhttps://elifesciences.org/articles/70676https://doaj.org/toc/2050-084XHistory, chance, and selection are the fundamental factors that drive and constrain evolution. We designed evolution experiments to disentangle and quantify effects of these forces on the evolution of antibiotic resistance. Previously, we showed that selection of the pathogen Acinetobacter baumannii in both structured and unstructured environments containing the antibiotic ciprofloxacin produced distinct genotypes and phenotypes, with lower resistance in biofilms as well as collateral sensitivity to β-lactam drugs (Santos-Lopez et al., 2019). Here we study how this prior history influences subsequent evolution in new β-lactam antibiotics. Selection was imposed by increasing concentrations of ceftazidime and imipenem and chance differences arose as random mutations among replicate populations. The effects of history were reduced by increasingly strong selection in new drugs, but not erased, at times revealing important contingencies. A history of selection in structured environments constrained resistance to new drugs and led to frequent loss of resistance to the initial drug by genetic reversions and not compensatory mutations. This research demonstrates that despite strong selective pressures of antibiotics leading to genetic parallelism, history can etch potential vulnerabilities to orthogonal drugs.Alfonso Santos-LopezChristopher W MarshallAllison L HaasCaroline TurnerJavier RaseroVaughn S CoopereLife Sciences Publications Ltdarticleacinetobactercollateral sensitivitypopulation geneticsgenomicseffluxMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acinetobacter collateral sensitivity population genetics genomics efflux Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	acinetobacter collateral sensitivity population genetics genomics efflux Medicine R Science Q Biology (General) QH301-705.5 Alfonso Santos-Lopez Christopher W Marshall Allison L Haas Caroline Turner Javier Rasero Vaughn S Cooper The roles of history, chance, and natural selection in the evolution of antibiotic resistance
description	History, chance, and selection are the fundamental factors that drive and constrain evolution. We designed evolution experiments to disentangle and quantify effects of these forces on the evolution of antibiotic resistance. Previously, we showed that selection of the pathogen Acinetobacter baumannii in both structured and unstructured environments containing the antibiotic ciprofloxacin produced distinct genotypes and phenotypes, with lower resistance in biofilms as well as collateral sensitivity to β-lactam drugs (Santos-Lopez et al., 2019). Here we study how this prior history influences subsequent evolution in new β-lactam antibiotics. Selection was imposed by increasing concentrations of ceftazidime and imipenem and chance differences arose as random mutations among replicate populations. The effects of history were reduced by increasingly strong selection in new drugs, but not erased, at times revealing important contingencies. A history of selection in structured environments constrained resistance to new drugs and led to frequent loss of resistance to the initial drug by genetic reversions and not compensatory mutations. This research demonstrates that despite strong selective pressures of antibiotics leading to genetic parallelism, history can etch potential vulnerabilities to orthogonal drugs.
format	article
author	Alfonso Santos-Lopez Christopher W Marshall Allison L Haas Caroline Turner Javier Rasero Vaughn S Cooper
author_facet	Alfonso Santos-Lopez Christopher W Marshall Allison L Haas Caroline Turner Javier Rasero Vaughn S Cooper
author_sort	Alfonso Santos-Lopez
title	The roles of history, chance, and natural selection in the evolution of antibiotic resistance
title_short	The roles of history, chance, and natural selection in the evolution of antibiotic resistance
title_full	The roles of history, chance, and natural selection in the evolution of antibiotic resistance
title_fullStr	The roles of history, chance, and natural selection in the evolution of antibiotic resistance
title_full_unstemmed	The roles of history, chance, and natural selection in the evolution of antibiotic resistance
title_sort	roles of history, chance, and natural selection in the evolution of antibiotic resistance
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/a885931ec4fa4f99b3d2d8a163af2398
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The roles of history, chance, and natural selection in the evolution of antibiotic resistance

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