Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells

The excellent photothermal properties of gold nanorods (Au-NRs) make them one of the most researched plasmonic photothermal nanomaterials. However, their biological applications have been hampered greatly due to surfactant-induced cytotoxicity. We herein report a simple synthesis of highly biocompat...

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Autores principales:	Adewale Oladipo, Thabang Calvin Lebepe, Sundararajan Parani, Rodney Maluleke, Vuyelwa Ncapayi, Grace It Mwad Mbaz, Sandile Phinda Songca, Tetsuya Kodama, Oluwatobi Samuel Oluwafemi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gold nanorods gelatin near-infrared stability cytotoxicity photothermal therapy Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/a88af0ca6f8b4c39bfc0d44199de71df
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spelling	oai:doaj.org-article:a88af0ca6f8b4c39bfc0d44199de71df2021-11-25T18:39:38ZSynthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells10.3390/ph141111371424-8247https://doaj.org/article/a88af0ca6f8b4c39bfc0d44199de71df2021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1137https://doaj.org/toc/1424-8247The excellent photothermal properties of gold nanorods (Au-NRs) make them one of the most researched plasmonic photothermal nanomaterials. However, their biological applications have been hampered greatly due to surfactant-induced cytotoxicity. We herein report a simple synthesis of highly biocompatible gelatin stabilized Au-NRs (gelatin@Au-NRs) to address this issue. The optical and structural properties of the as-synthesized gelatin@Au-NRs were investigated by Zetasizer, Ultraviolet-Visible-Near Infrared (UV-Vis-NIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and Fourier transform infrared spectroscopy (FTIR). The as-synthesized gelatin@Au-NRs were highly crystalline and rod-like in shape with an average length and diameter of 66.2 ± 2.3 nm and 10 ± 1.6 nm, respectively. The as-synthesized gelatin@Au-NRs showed high stability in common biological media (phosphate buffer saline and Dulbecco’s Modified Eagle’s Medium) compared to CTAB capped Au-NRs. Similarly, the gelatin@Au-NRs showed an improved heat production and outstanding cell viability against two different cancer cell lines; KM-Luc/GFP (mouse fibroblast histiocytoma cell line) and FM3A-Luc (breast carcinoma cell line) compared to CTAB capped Au-NRs and PEG@Au-NRs. An in vitro photothermal therapy study against KM-Luc/GFP showed that gelatin@Au-NRs effectively destroys the cancer cells.Adewale OladipoThabang Calvin LebepeSundararajan ParaniRodney MalulekeVuyelwa NcapayiGrace It Mwad MbazSandile Phinda SongcaTetsuya KodamaOluwatobi Samuel OluwafemiMDPI AGarticlegold nanorodsgelatinnear-infraredstabilitycytotoxicityphotothermal therapyMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1137, p 1137 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gold nanorods gelatin near-infrared stability cytotoxicity photothermal therapy Medicine R Pharmacy and materia medica RS1-441
spellingShingle	gold nanorods gelatin near-infrared stability cytotoxicity photothermal therapy Medicine R Pharmacy and materia medica RS1-441 Adewale Oladipo Thabang Calvin Lebepe Sundararajan Parani Rodney Maluleke Vuyelwa Ncapayi Grace It Mwad Mbaz Sandile Phinda Songca Tetsuya Kodama Oluwatobi Samuel Oluwafemi Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells
description	The excellent photothermal properties of gold nanorods (Au-NRs) make them one of the most researched plasmonic photothermal nanomaterials. However, their biological applications have been hampered greatly due to surfactant-induced cytotoxicity. We herein report a simple synthesis of highly biocompatible gelatin stabilized Au-NRs (gelatin@Au-NRs) to address this issue. The optical and structural properties of the as-synthesized gelatin@Au-NRs were investigated by Zetasizer, Ultraviolet-Visible-Near Infrared (UV-Vis-NIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and Fourier transform infrared spectroscopy (FTIR). The as-synthesized gelatin@Au-NRs were highly crystalline and rod-like in shape with an average length and diameter of 66.2 ± 2.3 nm and 10 ± 1.6 nm, respectively. The as-synthesized gelatin@Au-NRs showed high stability in common biological media (phosphate buffer saline and Dulbecco’s Modified Eagle’s Medium) compared to CTAB capped Au-NRs. Similarly, the gelatin@Au-NRs showed an improved heat production and outstanding cell viability against two different cancer cell lines; KM-Luc/GFP (mouse fibroblast histiocytoma cell line) and FM3A-Luc (breast carcinoma cell line) compared to CTAB capped Au-NRs and PEG@Au-NRs. An in vitro photothermal therapy study against KM-Luc/GFP showed that gelatin@Au-NRs effectively destroys the cancer cells.
format	article
author	Adewale Oladipo Thabang Calvin Lebepe Sundararajan Parani Rodney Maluleke Vuyelwa Ncapayi Grace It Mwad Mbaz Sandile Phinda Songca Tetsuya Kodama Oluwatobi Samuel Oluwafemi
author_facet	Adewale Oladipo Thabang Calvin Lebepe Sundararajan Parani Rodney Maluleke Vuyelwa Ncapayi Grace It Mwad Mbaz Sandile Phinda Songca Tetsuya Kodama Oluwatobi Samuel Oluwafemi
author_sort	Adewale Oladipo
title	Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells
title_short	Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells
title_full	Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells
title_fullStr	Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells
title_full_unstemmed	Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells
title_sort	synthesis of nir-ii absorbing gelatin stabilized gold nanorods and its photothermal therapy application against fibroblast histiocytoma cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a88af0ca6f8b4c39bfc0d44199de71df
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Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells

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