Distinct dendritic Ca2+ spike forms produce opposing input-output transformations in rat CA3 pyramidal cells

Proper integration of different inputs targeting the dendritic tree of CA3 pyramidal cells (CA3PCs) is critical for associative learning and recall. Dendritic Ca2+ spikes have been proposed to perform associative computations in other PC types by detecting conjunctive activation of different afferen...

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Autores principales:	Ádám Magó, Noémi Kis, Balázs Lükő, Judit K Makara
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	dendrite hippocampus CA3 pyramidal cell Ca2+ spike action potential burst Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a88b6144699e473590b1d782a233fae6
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id	oai:doaj.org-article:a88b6144699e473590b1d782a233fae6
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spelling	oai:doaj.org-article:a88b6144699e473590b1d782a233fae62021-11-24T16:07:46ZDistinct dendritic Ca2+ spike forms produce opposing input-output transformations in rat CA3 pyramidal cells10.7554/eLife.744932050-084Xe74493https://doaj.org/article/a88b6144699e473590b1d782a233fae62021-11-01T00:00:00Zhttps://elifesciences.org/articles/74493https://doaj.org/toc/2050-084XProper integration of different inputs targeting the dendritic tree of CA3 pyramidal cells (CA3PCs) is critical for associative learning and recall. Dendritic Ca2+ spikes have been proposed to perform associative computations in other PC types by detecting conjunctive activation of different afferent input pathways, initiating afterdepolarization (ADP), and triggering burst firing. Implementation of such operations fundamentally depends on the actual biophysical properties of dendritic Ca2+ spikes; yet little is known about these properties in dendrites of CA3PCs. Using dendritic patch-clamp recordings and two-photon Ca2+ imaging in acute slices from male rats, we report that, unlike CA1PCs, distal apical trunk dendrites of CA3PCs exhibit distinct forms of dendritic Ca2+ spikes. Besides ADP-type global Ca2+ spikes, a majority of dendrites expresses a novel, fast Ca2+ spike type that is initiated locally without bAPs, can recruit additional Na+ currents, and is compartmentalized to the activated dendritic subtree. Occurrence of the different Ca2+ spike types correlates with dendritic structure, indicating morpho-functional heterogeneity among CA3PCs. Importantly, ADPs and dendritically initiated spikes produce opposing somatic output: bursts versus strictly single-action potentials, respectively. The uncovered variability of dendritic Ca2+ spikes may underlie heterogeneous input-output transformation and bursting properties of CA3PCs, and might specifically contribute to key associative and non-associative computations performed by the CA3 network.Ádám MagóNoémi KisBalázs LükőJudit K MakaraeLife Sciences Publications LtdarticledendritehippocampusCA3 pyramidal cellCa2+ spikeaction potentialburstMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dendrite hippocampus CA3 pyramidal cell Ca2+ spike action potential burst Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	dendrite hippocampus CA3 pyramidal cell Ca2+ spike action potential burst Medicine R Science Q Biology (General) QH301-705.5 Ádám Magó Noémi Kis Balázs Lükő Judit K Makara Distinct dendritic Ca2+ spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
description	Proper integration of different inputs targeting the dendritic tree of CA3 pyramidal cells (CA3PCs) is critical for associative learning and recall. Dendritic Ca2+ spikes have been proposed to perform associative computations in other PC types by detecting conjunctive activation of different afferent input pathways, initiating afterdepolarization (ADP), and triggering burst firing. Implementation of such operations fundamentally depends on the actual biophysical properties of dendritic Ca2+ spikes; yet little is known about these properties in dendrites of CA3PCs. Using dendritic patch-clamp recordings and two-photon Ca2+ imaging in acute slices from male rats, we report that, unlike CA1PCs, distal apical trunk dendrites of CA3PCs exhibit distinct forms of dendritic Ca2+ spikes. Besides ADP-type global Ca2+ spikes, a majority of dendrites expresses a novel, fast Ca2+ spike type that is initiated locally without bAPs, can recruit additional Na+ currents, and is compartmentalized to the activated dendritic subtree. Occurrence of the different Ca2+ spike types correlates with dendritic structure, indicating morpho-functional heterogeneity among CA3PCs. Importantly, ADPs and dendritically initiated spikes produce opposing somatic output: bursts versus strictly single-action potentials, respectively. The uncovered variability of dendritic Ca2+ spikes may underlie heterogeneous input-output transformation and bursting properties of CA3PCs, and might specifically contribute to key associative and non-associative computations performed by the CA3 network.
format	article
author	Ádám Magó Noémi Kis Balázs Lükő Judit K Makara
author_facet	Ádám Magó Noémi Kis Balázs Lükő Judit K Makara
author_sort	Ádám Magó
title	Distinct dendritic Ca2+ spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
title_short	Distinct dendritic Ca2+ spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
title_full	Distinct dendritic Ca2+ spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
title_fullStr	Distinct dendritic Ca2+ spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
title_full_unstemmed	Distinct dendritic Ca2+ spike forms produce opposing input-output transformations in rat CA3 pyramidal cells
title_sort	distinct dendritic ca2+ spike forms produce opposing input-output transformations in rat ca3 pyramidal cells
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/a88b6144699e473590b1d782a233fae6
work_keys_str_mv	AT adammago distinctdendriticca2spikeformsproduceopposinginputoutputtransformationsinratca3pyramidalcells AT noemikis distinctdendriticca2spikeformsproduceopposinginputoutputtransformationsinratca3pyramidalcells AT balazsluko distinctdendriticca2spikeformsproduceopposinginputoutputtransformationsinratca3pyramidalcells AT juditkmakara distinctdendriticca2spikeformsproduceopposinginputoutputtransformationsinratca3pyramidalcells
_version_	1718414830967193600

Distinct dendritic Ca2+ spike forms produce opposing input-output transformations in rat CA3 pyramidal cells

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