Metabolic Changes in the Trichomes of Cannabis sativa var. bedrobinol Analyzed by 1H-NMR-Based Metabolomics

Trichomes of Cannabis sativa are the main tissue for synthesizing and storing cannabinoids, the most interesting compounds in this plant. In this report, metabolic changes in the trichomes of C. sativa var. bedrobinol were investigated by 1H-NMR-based metabolomics over the flowering session. Three c...

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Autores principales:	Nizar Happyana, Oliver Kayser
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2020
Materias:	cannabis cannabinoids 1h-nmr-based metabolomics trichomes Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/a8913c291efc40dc93e7888a95b33f76
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spelling	oai:doaj.org-article:a8913c291efc40dc93e7888a95b33f762021-12-02T12:53:43ZMetabolic Changes in the Trichomes of <i>Cannabis sativa</i> var. <i>bedrobinol</i> Analyzed by <sup>1</sup>H-NMR-Based Metabolomics1411-94202460-157810.22146/ijc.48765https://doaj.org/article/a8913c291efc40dc93e7888a95b33f762020-03-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/48765https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578Trichomes of Cannabis sativa are the main tissue for synthesizing and storing cannabinoids, the most interesting compounds in this plant. In this report, metabolic changes in the trichomes of C. sativa var. bedrobinol were investigated by 1H-NMR-based metabolomics over the flowering session. Three cannabinoids, including Δ9-tetrahydrocannabinolic acid (THCA), cannabichromenic acid (CBCA), and Δ9-tetrahydrocannabinol (THC), were successfully identified in the chloroform extracts of the Cannabis trichomes. Meanwhile, 20 non-cannabinoid compounds, including sugars, amino acids, and other acidic constituents, were detected in the water extracts. Metabolic changes of the Cannabis trichomes during the monitoring time were successfully revealed using the models of partial least squares discriminant analysis (PLSDA) and 1H-NMR quantitative analysis. Score plots of the PLSDA models classified metabolomes based on the harvest time. Discriminant metabolites for the differentiation were detected in the loading plots of the models. THCA was found as an important discriminant compound in the chloroform extracts, while all quantified water-soluble compounds were detected, contributing to the metabolic changes of the water extracts. The obtained results shed more light on the biosynthesis of metabolites in the Cannabis trichomes over the flowering season.Nizar HappyanaOliver KayserDepartment of Chemistry, Universitas Gadjah Madaarticlecannabiscannabinoids1h-nmr-based metabolomicstrichomesChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 20, Iss 6, Pp 1246-1254 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cannabis cannabinoids 1h-nmr-based metabolomics trichomes Chemistry QD1-999
spellingShingle	cannabis cannabinoids 1h-nmr-based metabolomics trichomes Chemistry QD1-999 Nizar Happyana Oliver Kayser Metabolic Changes in the Trichomes of <i>Cannabis sativa</i> var. <i>bedrobinol</i> Analyzed by <sup>1</sup>H-NMR-Based Metabolomics
description	Trichomes of Cannabis sativa are the main tissue for synthesizing and storing cannabinoids, the most interesting compounds in this plant. In this report, metabolic changes in the trichomes of C. sativa var. bedrobinol were investigated by 1H-NMR-based metabolomics over the flowering session. Three cannabinoids, including Δ9-tetrahydrocannabinolic acid (THCA), cannabichromenic acid (CBCA), and Δ9-tetrahydrocannabinol (THC), were successfully identified in the chloroform extracts of the Cannabis trichomes. Meanwhile, 20 non-cannabinoid compounds, including sugars, amino acids, and other acidic constituents, were detected in the water extracts. Metabolic changes of the Cannabis trichomes during the monitoring time were successfully revealed using the models of partial least squares discriminant analysis (PLSDA) and 1H-NMR quantitative analysis. Score plots of the PLSDA models classified metabolomes based on the harvest time. Discriminant metabolites for the differentiation were detected in the loading plots of the models. THCA was found as an important discriminant compound in the chloroform extracts, while all quantified water-soluble compounds were detected, contributing to the metabolic changes of the water extracts. The obtained results shed more light on the biosynthesis of metabolites in the Cannabis trichomes over the flowering season.
format	article
author	Nizar Happyana Oliver Kayser
author_facet	Nizar Happyana Oliver Kayser
author_sort	Nizar Happyana
title	Metabolic Changes in the Trichomes of <i>Cannabis sativa</i> var. <i>bedrobinol</i> Analyzed by <sup>1</sup>H-NMR-Based Metabolomics
title_short	Metabolic Changes in the Trichomes of <i>Cannabis sativa</i> var. <i>bedrobinol</i> Analyzed by <sup>1</sup>H-NMR-Based Metabolomics
title_full	Metabolic Changes in the Trichomes of <i>Cannabis sativa</i> var. <i>bedrobinol</i> Analyzed by <sup>1</sup>H-NMR-Based Metabolomics
title_fullStr	Metabolic Changes in the Trichomes of <i>Cannabis sativa</i> var. <i>bedrobinol</i> Analyzed by <sup>1</sup>H-NMR-Based Metabolomics
title_full_unstemmed	Metabolic Changes in the Trichomes of <i>Cannabis sativa</i> var. <i>bedrobinol</i> Analyzed by <sup>1</sup>H-NMR-Based Metabolomics
title_sort	metabolic changes in the trichomes of <i>cannabis sativa</i> var. <i>bedrobinol</i> analyzed by <sup>1</sup>h-nmr-based metabolomics
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2020
url	https://doaj.org/article/a8913c291efc40dc93e7888a95b33f76
work_keys_str_mv	AT nizarhappyana metabolicchangesinthetrichomesoficannabissativaivaribedrobinolianalyzedbysup1suphnmrbasedmetabolomics AT oliverkayser metabolicchangesinthetrichomesoficannabissativaivaribedrobinolianalyzedbysup1suphnmrbasedmetabolomics
_version_	1718393586060361728

Metabolic Changes in the Trichomes of <i>Cannabis sativa</i> var. <i>bedrobinol</i> Analyzed by <sup>1</sup>H-NMR-Based Metabolomics

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