miR-148 regulates Mitf in melanoma cells.

The Microphthalmia associated transcription factor (Mitf) is an important regulator in melanocyte development and has been shown to be involved in melanoma progression. The current model for the role of Mitf in melanoma assumes that the total activity of the protein is tightly regulated in order to...

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Autores principales:	Benedikta S Haflidadóttir, Kristín Bergsteinsdóttir, Christian Praetorius, Eiríkur Steingrímsson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a89a9913fd1944f19281e85994fcd7c8
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spelling	oai:doaj.org-article:a89a9913fd1944f19281e85994fcd7c82021-12-02T20:20:04ZmiR-148 regulates Mitf in melanoma cells.1932-620310.1371/journal.pone.0011574https://doaj.org/article/a89a9913fd1944f19281e85994fcd7c82010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20644734/?tool=EBIhttps://doaj.org/toc/1932-6203The Microphthalmia associated transcription factor (Mitf) is an important regulator in melanocyte development and has been shown to be involved in melanoma progression. The current model for the role of Mitf in melanoma assumes that the total activity of the protein is tightly regulated in order to secure cell proliferation. Previous research has shown that regulation of Mitf is complex and involves regulation of expression, splicing, protein stability and post-translational modifications. Here we show that microRNAs (miRNAs) are also involved in regulating Mitf in melanoma cells. Sequence analysis revealed conserved binding sites for several miRNAs in the Mitf 3'UTR sequence. Furthermore, miR-148 was shown to affect Mitf mRNA expression in melanoma cells through a conserved binding site in the 3'UTR sequence of mouse and human Mitf. In addition we confirm the previously reported effects of miR-137 on Mitf. Other miRNAs, miR-27a, miR-32 and miR-124 which all have conserved binding sites in the Mitf 3'UTR sequence did not have effects on Mitf. Our data show that miR-148 and miR-137 present an additional level of regulating Mitf expression in melanocytes and melanoma cells. Loss of this regulation, either by mutations or by shortening of the 3'UTR sequence, is therefore a likely factor in melanoma formation and/or progression.Benedikta S HaflidadóttirKristín BergsteinsdóttirChristian PraetoriusEiríkur SteingrímssonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11574 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Benedikta S Haflidadóttir Kristín Bergsteinsdóttir Christian Praetorius Eiríkur Steingrímsson miR-148 regulates Mitf in melanoma cells.
description	The Microphthalmia associated transcription factor (Mitf) is an important regulator in melanocyte development and has been shown to be involved in melanoma progression. The current model for the role of Mitf in melanoma assumes that the total activity of the protein is tightly regulated in order to secure cell proliferation. Previous research has shown that regulation of Mitf is complex and involves regulation of expression, splicing, protein stability and post-translational modifications. Here we show that microRNAs (miRNAs) are also involved in regulating Mitf in melanoma cells. Sequence analysis revealed conserved binding sites for several miRNAs in the Mitf 3'UTR sequence. Furthermore, miR-148 was shown to affect Mitf mRNA expression in melanoma cells through a conserved binding site in the 3'UTR sequence of mouse and human Mitf. In addition we confirm the previously reported effects of miR-137 on Mitf. Other miRNAs, miR-27a, miR-32 and miR-124 which all have conserved binding sites in the Mitf 3'UTR sequence did not have effects on Mitf. Our data show that miR-148 and miR-137 present an additional level of regulating Mitf expression in melanocytes and melanoma cells. Loss of this regulation, either by mutations or by shortening of the 3'UTR sequence, is therefore a likely factor in melanoma formation and/or progression.
format	article
author	Benedikta S Haflidadóttir Kristín Bergsteinsdóttir Christian Praetorius Eiríkur Steingrímsson
author_facet	Benedikta S Haflidadóttir Kristín Bergsteinsdóttir Christian Praetorius Eiríkur Steingrímsson
author_sort	Benedikta S Haflidadóttir
title	miR-148 regulates Mitf in melanoma cells.
title_short	miR-148 regulates Mitf in melanoma cells.
title_full	miR-148 regulates Mitf in melanoma cells.
title_fullStr	miR-148 regulates Mitf in melanoma cells.
title_full_unstemmed	miR-148 regulates Mitf in melanoma cells.
title_sort	mir-148 regulates mitf in melanoma cells.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/a89a9913fd1944f19281e85994fcd7c8
work_keys_str_mv	AT benediktashaflidadottir mir148regulatesmitfinmelanomacells AT kristinbergsteinsdottir mir148regulatesmitfinmelanomacells AT christianpraetorius mir148regulatesmitfinmelanomacells AT eirikursteingrimsson mir148regulatesmitfinmelanomacells
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miR-148 regulates Mitf in melanoma cells.

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