Efficient gene delivery to human umbilical cord mesenchymal stem cells by cationized Porphyra yezoensis polysaccharide nanoparticles

Qingtong Yu,1,2 Jin Cao,2 Baoding Chen,3 Wenwen Deng,2 Xia Cao,2 Jingjing Chen,2 Yan Wang,2 Shicheng Wang,2 Jiangnan Yu,2 Ximing Xu,2 Xiangdong Gao1 1School of Life Science and Technology, China Pharmaceutical University, Nanjing, 2Department of Pharmaceutics, School of Pharmacy and Center for Drug...

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Autores principales:	Yu Q, Cao J, Chen B, Deng W, Cao X, Chen J, Wang Y, Wang S, Yu J, Xu X, Gao X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a8a91d89a53b4430b7b4780ce100b325
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spelling	oai:doaj.org-article:a8a91d89a53b4430b7b4780ce100b3252021-12-02T06:46:22ZEfficient gene delivery to human umbilical cord mesenchymal stem cells by cationized Porphyra yezoensis polysaccharide nanoparticles1178-2013https://doaj.org/article/a8a91d89a53b4430b7b4780ce100b3252015-11-01T00:00:00Zhttps://www.dovepress.com/efficient-gene-delivery-to-human-umbilical-cord-mesenchymal-stem-cells-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Qingtong Yu,1,2 Jin Cao,2 Baoding Chen,3 Wenwen Deng,2 Xia Cao,2 Jingjing Chen,2 Yan Wang,2 Shicheng Wang,2 Jiangnan Yu,2 Ximing Xu,2 Xiangdong Gao1 1School of Life Science and Technology, China Pharmaceutical University, Nanjing, 2Department of Pharmaceutics, School of Pharmacy and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu University, 3Department of Medical Ultrasound, Affiliated Hospital of Jiangsu University, Zhenjiang, People’s Republic of China Abstract: This study centered on an innovative application of Porphyra yezoensis polysaccharide (PPS) with cationic modification as a safe and efficient nonviral gene vector to deliver a plasmid encoding human Wnt3a (pWnt3a) into human umbilical cord mesenchymal stem cells (HUMSCs). After modification with branched low-molecular-weight (1,200 Da) polyethylenimine, the cationized PPS (CPPS) was combined with pWnt3a to form spherical nanoscale particles (CPPS-pWnt3a nanoparticles). Particle size and distribution indicated that the CPPS-pWnt3a nanoparticles at a CPPS:pWnt3a weight ratio of 40:1 might be a potential candidate for DNA plasmid transfection. A cytotoxicity assay demonstrated that the nanoparticles prepared at a CPPS:pWnt3a weight ratio of 40:1 were nontoxic to HUMSCs compared to those of Lipofectamine 2000 and polyethylenimine (25 kDa). These nanoparticles were further transfected to HUMSCs. Western blotting demonstrated that the nanoparticles (CPPS:pWnt3a weight ratio 40:1) had the greatest transfection efficiency in HUMSCs, which was significantly higher than that of Lipofectamine 2000; however, when the CPPS:pWnt3a weight ratio was increased to 80:1, the nanoparticle-treated group showed no obvious improvement in translation efficiency over Lipofectamine 2000. Therefore, CPPS, a novel cationic polysaccharide derived from P. yezoensis, could be developed into a safe, efficient, nonviral gene vector in a gene-delivery system. Keywords: Porphyra yezoensis, nanoparticle, nonviral vector, human umbilical cord mesenchymal stem cells, Wnt3aYu QCao JChen BDeng WCao XChen JWang YWang SYu JXu XGao XDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7097-7107 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yu Q Cao J Chen B Deng W Cao X Chen J Wang Y Wang S Yu J Xu X Gao X Efficient gene delivery to human umbilical cord mesenchymal stem cells by cationized Porphyra yezoensis polysaccharide nanoparticles
description	Qingtong Yu,1,2 Jin Cao,2 Baoding Chen,3 Wenwen Deng,2 Xia Cao,2 Jingjing Chen,2 Yan Wang,2 Shicheng Wang,2 Jiangnan Yu,2 Ximing Xu,2 Xiangdong Gao1 1School of Life Science and Technology, China Pharmaceutical University, Nanjing, 2Department of Pharmaceutics, School of Pharmacy and Center for Drug/Gene Delivery and Tissue Engineering, Jiangsu University, 3Department of Medical Ultrasound, Affiliated Hospital of Jiangsu University, Zhenjiang, People’s Republic of China Abstract: This study centered on an innovative application of Porphyra yezoensis polysaccharide (PPS) with cationic modification as a safe and efficient nonviral gene vector to deliver a plasmid encoding human Wnt3a (pWnt3a) into human umbilical cord mesenchymal stem cells (HUMSCs). After modification with branched low-molecular-weight (1,200 Da) polyethylenimine, the cationized PPS (CPPS) was combined with pWnt3a to form spherical nanoscale particles (CPPS-pWnt3a nanoparticles). Particle size and distribution indicated that the CPPS-pWnt3a nanoparticles at a CPPS:pWnt3a weight ratio of 40:1 might be a potential candidate for DNA plasmid transfection. A cytotoxicity assay demonstrated that the nanoparticles prepared at a CPPS:pWnt3a weight ratio of 40:1 were nontoxic to HUMSCs compared to those of Lipofectamine 2000 and polyethylenimine (25 kDa). These nanoparticles were further transfected to HUMSCs. Western blotting demonstrated that the nanoparticles (CPPS:pWnt3a weight ratio 40:1) had the greatest transfection efficiency in HUMSCs, which was significantly higher than that of Lipofectamine 2000; however, when the CPPS:pWnt3a weight ratio was increased to 80:1, the nanoparticle-treated group showed no obvious improvement in translation efficiency over Lipofectamine 2000. Therefore, CPPS, a novel cationic polysaccharide derived from P. yezoensis, could be developed into a safe, efficient, nonviral gene vector in a gene-delivery system. Keywords: Porphyra yezoensis, nanoparticle, nonviral vector, human umbilical cord mesenchymal stem cells, Wnt3a
format	article
author	Yu Q Cao J Chen B Deng W Cao X Chen J Wang Y Wang S Yu J Xu X Gao X
author_facet	Yu Q Cao J Chen B Deng W Cao X Chen J Wang Y Wang S Yu J Xu X Gao X
author_sort	Yu Q
title	Efficient gene delivery to human umbilical cord mesenchymal stem cells by cationized Porphyra yezoensis polysaccharide nanoparticles
title_short	Efficient gene delivery to human umbilical cord mesenchymal stem cells by cationized Porphyra yezoensis polysaccharide nanoparticles
title_full	Efficient gene delivery to human umbilical cord mesenchymal stem cells by cationized Porphyra yezoensis polysaccharide nanoparticles
title_fullStr	Efficient gene delivery to human umbilical cord mesenchymal stem cells by cationized Porphyra yezoensis polysaccharide nanoparticles
title_full_unstemmed	Efficient gene delivery to human umbilical cord mesenchymal stem cells by cationized Porphyra yezoensis polysaccharide nanoparticles
title_sort	efficient gene delivery to human umbilical cord mesenchymal stem cells by cationized porphyra yezoensis polysaccharide nanoparticles
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/a8a91d89a53b4430b7b4780ce100b325
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Efficient gene delivery to human umbilical cord mesenchymal stem cells by cationized Porphyra yezoensis polysaccharide nanoparticles

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