Grandmaternal cells in cord blood

Abstracts: Background: During pregnancy a feto-maternal exchange of cells through the placenta conducts to maternal microchimerism (Mc) in the child and fetal Mc in the mother. Because of this bidirectional traffic of cells, pregnant women have also acquired maternal cells in utero from their mothe...

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Autores principales:	Karlin R. Karlmark, Marina El Haddad, Xavier-Côme Donato, Gabriel V. Martin, Florence Bretelle, Nathalie Lesavre, Jean-François Cocallemen, Marielle Martin, Christophe Picard, Tiffany Albentosa, Jean Roudier, Raoul Desbriere, Nathalie C. Lambert
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cord blood grandmaternal microchimerism HLA compatibility three generations Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a8ab16279e7f43f798684e88bac4b0d9
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spelling	oai:doaj.org-article:a8ab16279e7f43f798684e88bac4b0d92021-11-28T04:33:30ZGrandmaternal cells in cord blood2352-396410.1016/j.ebiom.2021.103721https://doaj.org/article/a8ab16279e7f43f798684e88bac4b0d92021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352396421005156https://doaj.org/toc/2352-3964Abstracts: Background: During pregnancy a feto-maternal exchange of cells through the placenta conducts to maternal microchimerism (Mc) in the child and fetal Mc in the mother. Because of this bidirectional traffic of cells, pregnant women have also acquired maternal cells in utero from their mother and could transfer grandmaternal (GdM) cells to their child through the maternal bloodstream during pregnancy. Thus, cord blood (CB) samples could theoretically carry GdMMc. Nevertheless this has never been demonstrated. Methods: Using Human Leukocyte Antigen (HLA)-specific quantitative PCR assays on three-generation families, we were able to test 28 CB samples from healthy primigravid women for GdMMc in whole blood (WB) and isolated cells (PBMC, T, B, granulocytes, stem cells). Findings: Five CB samples (18%) had GdMMc which could not be confounded with maternal source, with quantities 100 fold lower than maternal Mc in WB and PBMC. Risk of aneuploidies and/or related invasive prenatal procedures significantly correlated with the presence of GdMMc in CB (p=0.024). Significantly decreased HLA compatibility was observed in three-generation families from CB samples carrying GdMMc (p=0.019). Interpretation: Transgenerational transfer of cells could have implications in immunology and evolution. Further analyses will be necessary to evaluate whether GdMMc in CB is a passive or immunologically active transfer and whether invasive prenatal procedures could trigger GdMMc. Funding: Provence-Alpes-Côte d'Azur APEX grant # 2012_06549E, 2012_11786F and 2014_03978) and the Foundation for Medical Research (FRM Grant #ING20140129045).Karlin R. KarlmarkMarina El HaddadXavier-Côme DonatoGabriel V. MartinFlorence BretelleNathalie LesavreJean-François CocallemenMarielle MartinChristophe PicardTiffany AlbentosaJean RoudierRaoul DesbriereNathalie C. LambertElsevierarticleCord bloodgrandmaternal microchimerismHLA compatibilitythree generationsMedicineRMedicine (General)R5-920ENEBioMedicine, Vol 74, Iss , Pp 103721- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cord blood grandmaternal microchimerism HLA compatibility three generations Medicine R Medicine (General) R5-920
spellingShingle	Cord blood grandmaternal microchimerism HLA compatibility three generations Medicine R Medicine (General) R5-920 Karlin R. Karlmark Marina El Haddad Xavier-Côme Donato Gabriel V. Martin Florence Bretelle Nathalie Lesavre Jean-François Cocallemen Marielle Martin Christophe Picard Tiffany Albentosa Jean Roudier Raoul Desbriere Nathalie C. Lambert Grandmaternal cells in cord blood
description	Abstracts: Background: During pregnancy a feto-maternal exchange of cells through the placenta conducts to maternal microchimerism (Mc) in the child and fetal Mc in the mother. Because of this bidirectional traffic of cells, pregnant women have also acquired maternal cells in utero from their mother and could transfer grandmaternal (GdM) cells to their child through the maternal bloodstream during pregnancy. Thus, cord blood (CB) samples could theoretically carry GdMMc. Nevertheless this has never been demonstrated. Methods: Using Human Leukocyte Antigen (HLA)-specific quantitative PCR assays on three-generation families, we were able to test 28 CB samples from healthy primigravid women for GdMMc in whole blood (WB) and isolated cells (PBMC, T, B, granulocytes, stem cells). Findings: Five CB samples (18%) had GdMMc which could not be confounded with maternal source, with quantities 100 fold lower than maternal Mc in WB and PBMC. Risk of aneuploidies and/or related invasive prenatal procedures significantly correlated with the presence of GdMMc in CB (p=0.024). Significantly decreased HLA compatibility was observed in three-generation families from CB samples carrying GdMMc (p=0.019). Interpretation: Transgenerational transfer of cells could have implications in immunology and evolution. Further analyses will be necessary to evaluate whether GdMMc in CB is a passive or immunologically active transfer and whether invasive prenatal procedures could trigger GdMMc. Funding: Provence-Alpes-Côte d'Azur APEX grant # 2012_06549E, 2012_11786F and 2014_03978) and the Foundation for Medical Research (FRM Grant #ING20140129045).
format	article
author	Karlin R. Karlmark Marina El Haddad Xavier-Côme Donato Gabriel V. Martin Florence Bretelle Nathalie Lesavre Jean-François Cocallemen Marielle Martin Christophe Picard Tiffany Albentosa Jean Roudier Raoul Desbriere Nathalie C. Lambert
author_facet	Karlin R. Karlmark Marina El Haddad Xavier-Côme Donato Gabriel V. Martin Florence Bretelle Nathalie Lesavre Jean-François Cocallemen Marielle Martin Christophe Picard Tiffany Albentosa Jean Roudier Raoul Desbriere Nathalie C. Lambert
author_sort	Karlin R. Karlmark
title	Grandmaternal cells in cord blood
title_short	Grandmaternal cells in cord blood
title_full	Grandmaternal cells in cord blood
title_fullStr	Grandmaternal cells in cord blood
title_full_unstemmed	Grandmaternal cells in cord blood
title_sort	grandmaternal cells in cord blood
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/a8ab16279e7f43f798684e88bac4b0d9
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Grandmaternal cells in cord blood

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