Grandmaternal cells in cord blood

Abstracts: Background: During pregnancy a feto-maternal exchange of cells through the placenta conducts to maternal microchimerism (Mc) in the child and fetal Mc in the mother. Because of this bidirectional traffic of cells, pregnant women have also acquired maternal cells in utero from their mothe...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Karlin R. Karlmark, Marina El Haddad, Xavier-Côme Donato, Gabriel V. Martin, Florence Bretelle, Nathalie Lesavre, Jean-François Cocallemen, Marielle Martin, Christophe Picard, Tiffany Albentosa, Jean Roudier, Raoul Desbriere, Nathalie C. Lambert
Formato: article
Lenguaje:EN
Publicado: Elsevier 2021
Materias:
R
Acceso en línea:https://doaj.org/article/a8ab16279e7f43f798684e88bac4b0d9
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:a8ab16279e7f43f798684e88bac4b0d9
record_format dspace
spelling oai:doaj.org-article:a8ab16279e7f43f798684e88bac4b0d92021-11-28T04:33:30ZGrandmaternal cells in cord blood2352-396410.1016/j.ebiom.2021.103721https://doaj.org/article/a8ab16279e7f43f798684e88bac4b0d92021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352396421005156https://doaj.org/toc/2352-3964Abstracts: Background: During pregnancy a feto-maternal exchange of cells through the placenta conducts to maternal microchimerism (Mc) in the child and fetal Mc in the mother. Because of this bidirectional traffic of cells, pregnant women have also acquired maternal cells in utero from their mother and could transfer grandmaternal (GdM) cells to their child through the maternal bloodstream during pregnancy. Thus, cord blood (CB) samples could theoretically carry GdMMc. Nevertheless this has never been demonstrated. Methods: Using Human Leukocyte Antigen (HLA)-specific quantitative PCR assays on three-generation families, we were able to test 28 CB samples from healthy primigravid women for GdMMc in whole blood (WB) and isolated cells (PBMC, T, B, granulocytes, stem cells). Findings: Five CB samples (18%) had GdMMc which could not be confounded with maternal source, with quantities 100 fold lower than maternal Mc in WB and PBMC. Risk of aneuploidies and/or related invasive prenatal procedures significantly correlated with the presence of GdMMc in CB (p=0.024). Significantly decreased HLA compatibility was observed in three-generation families from CB samples carrying GdMMc (p=0.019). Interpretation: Transgenerational transfer of cells could have implications in immunology and evolution. Further analyses will be necessary to evaluate whether GdMMc in CB is a passive or immunologically active transfer and whether invasive prenatal procedures could trigger GdMMc. Funding: Provence-Alpes-Côte d'Azur APEX grant # 2012_06549E, 2012_11786F and 2014_03978) and the Foundation for Medical Research (FRM Grant #ING20140129045).Karlin R. KarlmarkMarina El HaddadXavier-Côme DonatoGabriel V. MartinFlorence BretelleNathalie LesavreJean-François CocallemenMarielle MartinChristophe PicardTiffany AlbentosaJean RoudierRaoul DesbriereNathalie C. LambertElsevierarticleCord bloodgrandmaternal microchimerismHLA compatibilitythree generationsMedicineRMedicine (General)R5-920ENEBioMedicine, Vol 74, Iss , Pp 103721- (2021)
institution DOAJ
collection DOAJ
language EN
topic Cord blood
grandmaternal microchimerism
HLA compatibility
three generations
Medicine
R
Medicine (General)
R5-920
spellingShingle Cord blood
grandmaternal microchimerism
HLA compatibility
three generations
Medicine
R
Medicine (General)
R5-920
Karlin R. Karlmark
Marina El Haddad
Xavier-Côme Donato
Gabriel V. Martin
Florence Bretelle
Nathalie Lesavre
Jean-François Cocallemen
Marielle Martin
Christophe Picard
Tiffany Albentosa
Jean Roudier
Raoul Desbriere
Nathalie C. Lambert
Grandmaternal cells in cord blood
description Abstracts: Background: During pregnancy a feto-maternal exchange of cells through the placenta conducts to maternal microchimerism (Mc) in the child and fetal Mc in the mother. Because of this bidirectional traffic of cells, pregnant women have also acquired maternal cells in utero from their mother and could transfer grandmaternal (GdM) cells to their child through the maternal bloodstream during pregnancy. Thus, cord blood (CB) samples could theoretically carry GdMMc. Nevertheless this has never been demonstrated. Methods: Using Human Leukocyte Antigen (HLA)-specific quantitative PCR assays on three-generation families, we were able to test 28 CB samples from healthy primigravid women for GdMMc in whole blood (WB) and isolated cells (PBMC, T, B, granulocytes, stem cells). Findings: Five CB samples (18%) had GdMMc which could not be confounded with maternal source, with quantities 100 fold lower than maternal Mc in WB and PBMC. Risk of aneuploidies and/or related invasive prenatal procedures significantly correlated with the presence of GdMMc in CB (p=0.024). Significantly decreased HLA compatibility was observed in three-generation families from CB samples carrying GdMMc (p=0.019). Interpretation: Transgenerational transfer of cells could have implications in immunology and evolution. Further analyses will be necessary to evaluate whether GdMMc in CB is a passive or immunologically active transfer and whether invasive prenatal procedures could trigger GdMMc. Funding: Provence-Alpes-Côte d'Azur APEX grant # 2012_06549E, 2012_11786F and 2014_03978) and the Foundation for Medical Research (FRM Grant #ING20140129045).
format article
author Karlin R. Karlmark
Marina El Haddad
Xavier-Côme Donato
Gabriel V. Martin
Florence Bretelle
Nathalie Lesavre
Jean-François Cocallemen
Marielle Martin
Christophe Picard
Tiffany Albentosa
Jean Roudier
Raoul Desbriere
Nathalie C. Lambert
author_facet Karlin R. Karlmark
Marina El Haddad
Xavier-Côme Donato
Gabriel V. Martin
Florence Bretelle
Nathalie Lesavre
Jean-François Cocallemen
Marielle Martin
Christophe Picard
Tiffany Albentosa
Jean Roudier
Raoul Desbriere
Nathalie C. Lambert
author_sort Karlin R. Karlmark
title Grandmaternal cells in cord blood
title_short Grandmaternal cells in cord blood
title_full Grandmaternal cells in cord blood
title_fullStr Grandmaternal cells in cord blood
title_full_unstemmed Grandmaternal cells in cord blood
title_sort grandmaternal cells in cord blood
publisher Elsevier
publishDate 2021
url https://doaj.org/article/a8ab16279e7f43f798684e88bac4b0d9
work_keys_str_mv AT karlinrkarlmark grandmaternalcellsincordblood
AT marinaelhaddad grandmaternalcellsincordblood
AT xaviercomedonato grandmaternalcellsincordblood
AT gabrielvmartin grandmaternalcellsincordblood
AT florencebretelle grandmaternalcellsincordblood
AT nathalielesavre grandmaternalcellsincordblood
AT jeanfrancoiscocallemen grandmaternalcellsincordblood
AT mariellemartin grandmaternalcellsincordblood
AT christophepicard grandmaternalcellsincordblood
AT tiffanyalbentosa grandmaternalcellsincordblood
AT jeanroudier grandmaternalcellsincordblood
AT raouldesbriere grandmaternalcellsincordblood
AT nathalieclambert grandmaternalcellsincordblood
_version_ 1718408336953573376