Computational 4D-OCM for label-free imaging of collective cell invasion and force-mediated deformations in collagen

Abstract Traction force microscopy (TFM) is an important family of techniques used to measure and study the role of cellular traction forces (CTFs) associated with many biological processes. However, current standard TFM methods rely on imaging techniques that do not provide the experimental capabil...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jeffrey A. Mulligan, Lu Ling, Nichaluk Leartprapun, Claudia Fischbach, Steven G. Adie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a8c026d09ab44f1d9c51a8de9a67ff36
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a8c026d09ab44f1d9c51a8de9a67ff36
record_format	dspace
spelling	oai:doaj.org-article:a8c026d09ab44f1d9c51a8de9a67ff362021-12-02T14:06:31ZComputational 4D-OCM for label-free imaging of collective cell invasion and force-mediated deformations in collagen10.1038/s41598-021-81470-72045-2322https://doaj.org/article/a8c026d09ab44f1d9c51a8de9a67ff362021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81470-7https://doaj.org/toc/2045-2322Abstract Traction force microscopy (TFM) is an important family of techniques used to measure and study the role of cellular traction forces (CTFs) associated with many biological processes. However, current standard TFM methods rely on imaging techniques that do not provide the experimental capabilities necessary to study CTFs within 3D collective and dynamic systems embedded within optically scattering media. Traction force optical coherence microscopy (TF-OCM) was developed to address these needs, but has only been demonstrated for the study of isolated cells embedded within optically clear media. Here, we present computational 4D-OCM methods that enable the study of dynamic invasion behavior of large tumor spheroids embedded in collagen. Our multi-day, time-lapse imaging data provided detailed visualizations of evolving spheroid morphology, collagen degradation, and collagen deformation, all using label-free scattering contrast. These capabilities, which provided insights into how stromal cells affect cancer progression, significantly expand access to critical data about biophysical interactions of cells with their environment, and lay the foundation for future efforts toward volumetric, time-lapse reconstructions of collective CTFs with TF-OCM.Jeffrey A. MulliganLu LingNichaluk LeartprapunClaudia FischbachSteven G. AdieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeffrey A. Mulligan Lu Ling Nichaluk Leartprapun Claudia Fischbach Steven G. Adie Computational 4D-OCM for label-free imaging of collective cell invasion and force-mediated deformations in collagen
description	Abstract Traction force microscopy (TFM) is an important family of techniques used to measure and study the role of cellular traction forces (CTFs) associated with many biological processes. However, current standard TFM methods rely on imaging techniques that do not provide the experimental capabilities necessary to study CTFs within 3D collective and dynamic systems embedded within optically scattering media. Traction force optical coherence microscopy (TF-OCM) was developed to address these needs, but has only been demonstrated for the study of isolated cells embedded within optically clear media. Here, we present computational 4D-OCM methods that enable the study of dynamic invasion behavior of large tumor spheroids embedded in collagen. Our multi-day, time-lapse imaging data provided detailed visualizations of evolving spheroid morphology, collagen degradation, and collagen deformation, all using label-free scattering contrast. These capabilities, which provided insights into how stromal cells affect cancer progression, significantly expand access to critical data about biophysical interactions of cells with their environment, and lay the foundation for future efforts toward volumetric, time-lapse reconstructions of collective CTFs with TF-OCM.
format	article
author	Jeffrey A. Mulligan Lu Ling Nichaluk Leartprapun Claudia Fischbach Steven G. Adie
author_facet	Jeffrey A. Mulligan Lu Ling Nichaluk Leartprapun Claudia Fischbach Steven G. Adie
author_sort	Jeffrey A. Mulligan
title	Computational 4D-OCM for label-free imaging of collective cell invasion and force-mediated deformations in collagen
title_short	Computational 4D-OCM for label-free imaging of collective cell invasion and force-mediated deformations in collagen
title_full	Computational 4D-OCM for label-free imaging of collective cell invasion and force-mediated deformations in collagen
title_fullStr	Computational 4D-OCM for label-free imaging of collective cell invasion and force-mediated deformations in collagen
title_full_unstemmed	Computational 4D-OCM for label-free imaging of collective cell invasion and force-mediated deformations in collagen
title_sort	computational 4d-ocm for label-free imaging of collective cell invasion and force-mediated deformations in collagen
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a8c026d09ab44f1d9c51a8de9a67ff36
work_keys_str_mv	AT jeffreyamulligan computational4docmforlabelfreeimagingofcollectivecellinvasionandforcemediateddeformationsincollagen AT luling computational4docmforlabelfreeimagingofcollectivecellinvasionandforcemediateddeformationsincollagen AT nichalukleartprapun computational4docmforlabelfreeimagingofcollectivecellinvasionandforcemediateddeformationsincollagen AT claudiafischbach computational4docmforlabelfreeimagingofcollectivecellinvasionandforcemediateddeformationsincollagen AT stevengadie computational4docmforlabelfreeimagingofcollectivecellinvasionandforcemediateddeformationsincollagen
_version_	1718391981760053248

Computational 4D-OCM for label-free imaging of collective cell invasion and force-mediated deformations in collagen

Ejemplares similares